Mitotic and proliferative indices in who grade iii meningioma
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Mitotic and proliferative indices in who grade iii meningioma. / Maier, Andrea Daniela; Brøchner, Christian Beltoft; Bartek, Jiri; Eriksson, Frank; Ugleholdt, Heidi; Broholm, Helle; Mathiesen, Tiit.
In: Cancers, Vol. 12, No. 11, 3351, 2020, p. 1-10.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Mitotic and proliferative indices in who grade iii meningioma
AU - Maier, Andrea Daniela
AU - Brøchner, Christian Beltoft
AU - Bartek, Jiri
AU - Eriksson, Frank
AU - Ugleholdt, Heidi
AU - Broholm, Helle
AU - Mathiesen, Tiit
PY - 2020
Y1 - 2020
N2 - Meningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stained slides (PHH3 MI). This study investigates the agreement of MI and PHH3 MI and to analyze the association of progression-free survival (PFS) and MI, PHH3 MI, and the proliferative index (PI, Ki-67) in WHO grade III meningioma. Tumor specimens from 24 consecutive patients were analyzed for expression of Ki-67, PHH3 MI, and MI. Quantification was performed independently by two observers who made replicate counts in hot spots and overall tumor staining. Repeatability in replicate counts from MI and PHH3 MI was low in both observers. Consequently, we could not report the agreement. MI, PHH3 MI and hot spot counts of Ki-67 were associated with PFS (MI hot spot HR = 1.61, 95% CI 1.12–2.31, p = 0.010; PHH3 MI hot spot HR = 1.59, 95% CI 1.15–2.21, p = 0.006; Ki-67 hot spot HR = 1.06, 95% CI 1.02–1.11. p = 0.004). We found markedly low repeatability of manually counted MI and PHH3 MI in WHO grade III meningioma, and we could not conclude that the two methods agreed. Subsequently, quantification with better repeatability should be sought. All three biomarkers were associated with PFS.
AB - Meningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stained slides (PHH3 MI). This study investigates the agreement of MI and PHH3 MI and to analyze the association of progression-free survival (PFS) and MI, PHH3 MI, and the proliferative index (PI, Ki-67) in WHO grade III meningioma. Tumor specimens from 24 consecutive patients were analyzed for expression of Ki-67, PHH3 MI, and MI. Quantification was performed independently by two observers who made replicate counts in hot spots and overall tumor staining. Repeatability in replicate counts from MI and PHH3 MI was low in both observers. Consequently, we could not report the agreement. MI, PHH3 MI and hot spot counts of Ki-67 were associated with PFS (MI hot spot HR = 1.61, 95% CI 1.12–2.31, p = 0.010; PHH3 MI hot spot HR = 1.59, 95% CI 1.15–2.21, p = 0.006; Ki-67 hot spot HR = 1.06, 95% CI 1.02–1.11. p = 0.004). We found markedly low repeatability of manually counted MI and PHH3 MI in WHO grade III meningioma, and we could not conclude that the two methods agreed. Subsequently, quantification with better repeatability should be sought. All three biomarkers were associated with PFS.
KW - Agreement
KW - Hot spots
KW - Ki-67
KW - Malignant meningioma
KW - Mitotic indices
U2 - 10.3390/cancers12113351
DO - 10.3390/cancers12113351
M3 - Journal article
C2 - 33198268
AN - SCOPUS:85096652111
VL - 12
SP - 1
EP - 10
JO - Cancers
JF - Cancers
SN - 2072-6694
IS - 11
M1 - 3351
ER -
ID: 252721453