Reliability of three versus five saliva sampling times for assessing the cortisol awakening response

Research output: Contribution to journalJournal articlepeer-review

The cortisol awakening response (CAR) describes the sharp increase in cortisol secretion within 60 min after awakening. A summary of the CAR, the area under the cortisol curve above the awakening cortisol value (AUCi) is a widely used biomarker in health research. Estimation of the AUCi rely on a number of collected salivary samples at fixed time intervals (i.e., 5 samples in 15 min intervals) starting from awakening. Little empirical work has been executed to investigate the impact of reducing sampling times on AUCi estimation, which could potentially improve participant compliance and reduce operational costs. This study aimed to assess the reli-ability and validity of using 3-sample AUCi versus 5-sample AUCi, i.e., systematic and random fluctuations based on a large dataset from healthy and case individuals (total n = 537). We showed that the ideal timing of 3 -sam-pling times was 0-30-60 min with a median difference in AUCi of -8 nmol*h/L and interquartile range of 65 nmol*h/L among healthy individuals, and -12 nmol*h/L and 78 nmol*h/L among case individuals. We sub-sequently validated the 3-sample AUCi by re-analyzing three published association studies. Overall, we obtained similar p-values with 3-sample AUCi when compared to 5-sample AUCi, while smaller effect sizes and standard errors were observed. In conclusion, despite a less precise estimation of the AUCi itself, our data support that the AUC measure of the CAR, based on three samples collected at 0-30-60 min from awakening, provides reliable results in association studies.

Original languageEnglish
Article number105950
JournalPsychoneuroendocrinology
Volume147
Number of pages10
ISSN0306-4530
DOIs
Publication statusPublished - 2023

    Research areas

  • Endocrinology, Cortisol awakening response, Cortisol measurement, Cortisol indices, Stress hormone, Steroid hormone, HPA-axis, Positron Emission Tomography, UNDER-THE-CURVE, SEROTONIN TRANSPORTER, RECEPTOR-BINDING, BRAIN, INDICATORS, DEPRESSION, REACTIVITY, 5-HTTLPR, WAKING, MDMA

ID: 325819778