Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice
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Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice. / Gutierrez, Claudia Torero; Loizides, Charis; Hafez, Iosif; Brostrøm, Anders; Wolff, Henrik; Szarek, Józef; Berthing, Trine; Mortensen, Alicja; Jensen, Keld Alstrup; Roursgaard, Martin; Saber, Anne Thoustrup; Møller, Peter; Biskos, George; Vogel, Ulla.
In: Particle and Fibre Toxicology, Vol. 20, No. 1, 4, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Acute phase response following pulmonary exposure to soluble and insoluble metal oxide nanomaterials in mice
AU - Gutierrez, Claudia Torero
AU - Loizides, Charis
AU - Hafez, Iosif
AU - Brostrøm, Anders
AU - Wolff, Henrik
AU - Szarek, Józef
AU - Berthing, Trine
AU - Mortensen, Alicja
AU - Jensen, Keld Alstrup
AU - Roursgaard, Martin
AU - Saber, Anne Thoustrup
AU - Møller, Peter
AU - Biskos, George
AU - Vogel, Ulla
N1 - Publisher Copyright: © 2023, The Author(s).
PY - 2023
Y1 - 2023
N2 - Background: Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al2O3, SnO2 and TiO2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues. Results: All nanomaterials induced pulmonary inflammation after 1 day, and exposure to ZnO, CuO, SnO2, TiO2 and Printex 90 increased Saa3 mRNA levels in lungs and Saa1 mRNA levels in liver. Additionally, CuO, SnO2, TiO2 and Printex 90 increased plasma levels of SAA3 and SAA1/2. Acute phase response was predicted by deposited surface area for insoluble metal oxides, 1 and 28 days post-exposure. Conclusion: Soluble and insoluble metal oxides induced dose-dependent APR with different time dependency. Neutrophil influx, Saa3 mRNA levels in lung tissue and plasma SAA3 levels correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.
AB - Background: Acute phase response (APR) is characterized by a change in concentration of different proteins, including C-reactive protein and serum amyloid A (SAA) that can be linked to both exposure to metal oxide nanomaterials and risk of cardiovascular diseases. In this study, we intratracheally exposed mice to ZnO, CuO, Al2O3, SnO2 and TiO2 and carbon black (Printex 90) nanomaterials with a wide range in phagolysosomal solubility. We subsequently assessed neutrophil numbers, protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, Saa3 and Saa1 mRNA levels in lung and liver tissue, respectively, and SAA3 and SAA1/2 in plasma. Endpoints were analyzed 1 and 28 days after exposure, including histopathology of lung and liver tissues. Results: All nanomaterials induced pulmonary inflammation after 1 day, and exposure to ZnO, CuO, SnO2, TiO2 and Printex 90 increased Saa3 mRNA levels in lungs and Saa1 mRNA levels in liver. Additionally, CuO, SnO2, TiO2 and Printex 90 increased plasma levels of SAA3 and SAA1/2. Acute phase response was predicted by deposited surface area for insoluble metal oxides, 1 and 28 days post-exposure. Conclusion: Soluble and insoluble metal oxides induced dose-dependent APR with different time dependency. Neutrophil influx, Saa3 mRNA levels in lung tissue and plasma SAA3 levels correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.
KW - Acute phase response
KW - Metal oxide
KW - Nanomaterial
KW - Serum amyloid a
U2 - 10.1186/s12989-023-00514-0
DO - 10.1186/s12989-023-00514-0
M3 - Journal article
C2 - 36650530
AN - SCOPUS:85146405157
VL - 20
JO - Particle and Fibre Toxicology
JF - Particle and Fibre Toxicology
SN - 1743-8977
IS - 1
M1 - 4
ER -
ID: 334391921