Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells

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Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. / Løhr, Mille; Jensen, Annie; Eriksen, Louise; Grønbæk, Morten; Loft, Steffen; Møller, Peter.

In: Mutagenesis, Vol. 30, No. 5, 2015, p. 695-700.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Løhr, M, Jensen, A, Eriksen, L, Grønbæk, M, Loft, S & Møller, P 2015, 'Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells', Mutagenesis, vol. 30, no. 5, pp. 695-700. https://doi.org/10.1093/mutage/gev031

APA

Løhr, M., Jensen, A., Eriksen, L., Grønbæk, M., Loft, S., & Møller, P. (2015). Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. Mutagenesis, 30(5), 695-700. https://doi.org/10.1093/mutage/gev031

Vancouver

Løhr M, Jensen A, Eriksen L, Grønbæk M, Loft S, Møller P. Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. Mutagenesis. 2015;30(5):695-700. https://doi.org/10.1093/mutage/gev031

Author

Løhr, Mille ; Jensen, Annie ; Eriksen, Louise ; Grønbæk, Morten ; Loft, Steffen ; Møller, Peter. / Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells. In: Mutagenesis. 2015 ; Vol. 30, No. 5. pp. 695-700.

Bibtex

@article{3a25d4b4364f44d59debc9d957b2e5d2,
title = "Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells",
abstract = "It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65{\%} decline in activity per year from age 18 to 83 (95{\%} confidence interval: 0.16-1.14{\%} per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.",
author = "Mille L{\o}hr and Annie Jensen and Louise Eriksen and Morten Gr{\o}nb{\ae}k and Steffen Loft and Peter M{\o}ller",
note = "{\circledC} The Author 2015. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",
year = "2015",
doi = "10.1093/mutage/gev031",
language = "English",
volume = "30",
pages = "695--700",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Association between age and repair of oxidatively damaged DNA in human peripheral blood mononuclear cells

AU - Løhr, Mille

AU - Jensen, Annie

AU - Eriksen, Louise

AU - Grønbæk, Morten

AU - Loft, Steffen

AU - Møller, Peter

N1 - © The Author 2015. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2015

Y1 - 2015

N2 - It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.

AB - It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripheral blood mononuclear cells (PBMCs). We isolated PBMCs from subjects aged 18-83 years, as part of a health survey of the Danish population that focussed on lifestyle factors. The level of DNA repair activity was measured as incisions on potassium bromate-damaged DNA by the comet assay. There was an inverse association between age and DNA repair activity with a 0.65% decline in activity per year from age 18 to 83 (95% confidence interval: 0.16-1.14% per year). Univariate regression analysis also indicated inverse associations between DNA repair activity and waist-hip ratio (P < 0.05) and plasma concentrations of glycosylated hemoglobin (P = 0.07). However, multivariate regression analysis only showed an inverse association between age and DNA repair activity (P < 0.05), indicating that the decline in repair activity was not mediated by metabolic risk factors. In summary, the results show an inverse association between age and DNA repair activity of oxidatively damaged DNA.

U2 - 10.1093/mutage/gev031

DO - 10.1093/mutage/gev031

M3 - Journal article

C2 - 25925070

VL - 30

SP - 695

EP - 700

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 5

ER -

ID: 161061090