Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies

Research output: Contribution to journal › Review › Research › peer-review

Standard

Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies. / Møller, Peter; Bankoglu, Ezgi Eyluel; Stopper, Helga; Giovannelli, Lisa; Ladeira, Carina; Koppen, Gudrun; Gajski, Goran; Collins, Andrew; Valdiglesias, Vanessa; Laffon, Blanca; Boutet-Robinet, Elisa; Perdry, Herve; Del Bo', Cristian; Langie, Sabine A. S.; Dusinska, Maria; Azqueta, Amaya.

In: Mutagenesis, Vol. 36, No. 3, 2021, p. 193-212.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Møller, P, Bankoglu, EE, Stopper, H, Giovannelli, L, Ladeira, C, Koppen, G, Gajski, G, Collins, A, Valdiglesias, V, Laffon, B, Boutet-Robinet, E, Perdry, H, Del Bo', C, Langie, SAS, Dusinska, M & Azqueta, A 2021, 'Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies', Mutagenesis, vol. 36, no. 3, pp. 193-212. https://doi.org/10.1093/mutage/geab012

APA

Møller, P., Bankoglu, E. E., Stopper, H., Giovannelli, L., Ladeira, C., Koppen, G., Gajski, G., Collins, A., Valdiglesias, V., Laffon, B., Boutet-Robinet, E., Perdry, H., Del Bo', C., Langie, S. A. S., Dusinska, M., & Azqueta, A. (2021). Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies. Mutagenesis, 36(3), 193-212. https://doi.org/10.1093/mutage/geab012

Vancouver

Møller P, Bankoglu EE, Stopper H, Giovannelli L, Ladeira C, Koppen G et al. Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies. Mutagenesis. 2021;36(3):193-212. https://doi.org/10.1093/mutage/geab012

Author

Møller, Peter ; Bankoglu, Ezgi Eyluel ; Stopper, Helga ; Giovannelli, Lisa ; Ladeira, Carina ; Koppen, Gudrun ; Gajski, Goran ; Collins, Andrew ; Valdiglesias, Vanessa ; Laffon, Blanca ; Boutet-Robinet, Elisa ; Perdry, Herve ; Del Bo', Cristian ; Langie, Sabine A. S. ; Dusinska, Maria ; Azqueta, Amaya. / Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies. In: Mutagenesis. 2021 ; Vol. 36, No. 3. pp. 193-212.

Bibtex

@article{e0c05d15cca14e86903e66e32d4304d4,
title = "Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies",
abstract = "DNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyse the samples on the day of collection without any type of storage. For instance, certain studies use repeated sampling of cells from the same subject or samples from different subjects collected at different time-points, and it is desirable to analyse all these samples in the same comet assay experiment. In addition, flawless comet assay analyses on frozen samples open up the possibility of using this technique on biobank material. In this article we discuss the use of cryopreserved peripheral blood mononuclear cells (PBMCs), buffy coat (BC) and whole blood (WB) for analysis of DNA damage and repair using the comet assay. The published literature and the authors' experiences indicate that various types of blood samples can be cryopreserved with only a minor effect on the basal level of DNA damage. There is evidence to suggest that WB and PBMCs can be cryopreserved for several years without much effect on the level of DNA damage. However, care should be taken when cryopreserving WB and BCs. It is possible to use either fresh or frozen samples of blood cells, but results from fresh and frozen cells should not be used in the same dataset. The article outlines detailed protocols for the cryopreservation of PBMCs, BCs and WB samples.",
keywords = "BREAST-CANCER PATIENTS, CRYOPRESERVED LYMPHOCYTES, PERIPHERAL LYMPHOCYTES, GEL-ELECTROPHORESIS, SEASONAL-VARIATIONS, OXIDATIVE DAMAGE, MAMMALIAN-CELLS, WHOLE-BLOOD, IN-VITRO, VALIDATION",
author = "Peter M{\o}ller and Bankoglu, {Ezgi Eyluel} and Helga Stopper and Lisa Giovannelli and Carina Ladeira and Gudrun Koppen and Goran Gajski and Andrew Collins and Vanessa Valdiglesias and Blanca Laffon and Elisa Boutet-Robinet and Herve Perdry and {Del Bo'}, Cristian and Langie, {Sabine A. S.} and Maria Dusinska and Amaya Azqueta",
year = "2021",
doi = "10.1093/mutage/geab012",
language = "English",
volume = "36",
pages = "193--212",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies

AU - Møller, Peter

AU - Bankoglu, Ezgi Eyluel

AU - Stopper, Helga

AU - Giovannelli, Lisa

AU - Ladeira, Carina

AU - Koppen, Gudrun

AU - Gajski, Goran

AU - Collins, Andrew

AU - Valdiglesias, Vanessa

AU - Laffon, Blanca

AU - Boutet-Robinet, Elisa

AU - Perdry, Herve

AU - Del Bo', Cristian

AU - Langie, Sabine A. S.

AU - Dusinska, Maria

AU - Azqueta, Amaya

PY - 2021

Y1 - 2021

N2 - DNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyse the samples on the day of collection without any type of storage. For instance, certain studies use repeated sampling of cells from the same subject or samples from different subjects collected at different time-points, and it is desirable to analyse all these samples in the same comet assay experiment. In addition, flawless comet assay analyses on frozen samples open up the possibility of using this technique on biobank material. In this article we discuss the use of cryopreserved peripheral blood mononuclear cells (PBMCs), buffy coat (BC) and whole blood (WB) for analysis of DNA damage and repair using the comet assay. The published literature and the authors' experiences indicate that various types of blood samples can be cryopreserved with only a minor effect on the basal level of DNA damage. There is evidence to suggest that WB and PBMCs can be cryopreserved for several years without much effect on the level of DNA damage. However, care should be taken when cryopreserving WB and BCs. It is possible to use either fresh or frozen samples of blood cells, but results from fresh and frozen cells should not be used in the same dataset. The article outlines detailed protocols for the cryopreservation of PBMCs, BCs and WB samples.

AB - DNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyse the samples on the day of collection without any type of storage. For instance, certain studies use repeated sampling of cells from the same subject or samples from different subjects collected at different time-points, and it is desirable to analyse all these samples in the same comet assay experiment. In addition, flawless comet assay analyses on frozen samples open up the possibility of using this technique on biobank material. In this article we discuss the use of cryopreserved peripheral blood mononuclear cells (PBMCs), buffy coat (BC) and whole blood (WB) for analysis of DNA damage and repair using the comet assay. The published literature and the authors' experiences indicate that various types of blood samples can be cryopreserved with only a minor effect on the basal level of DNA damage. There is evidence to suggest that WB and PBMCs can be cryopreserved for several years without much effect on the level of DNA damage. However, care should be taken when cryopreserving WB and BCs. It is possible to use either fresh or frozen samples of blood cells, but results from fresh and frozen cells should not be used in the same dataset. The article outlines detailed protocols for the cryopreservation of PBMCs, BCs and WB samples.

KW - BREAST-CANCER PATIENTS

KW - CRYOPRESERVED LYMPHOCYTES

KW - PERIPHERAL LYMPHOCYTES

KW - GEL-ELECTROPHORESIS

KW - SEASONAL-VARIATIONS

KW - OXIDATIVE DAMAGE

KW - MAMMALIAN-CELLS

KW - WHOLE-BLOOD

KW - IN-VITRO

KW - VALIDATION

U2 - 10.1093/mutage/geab012

DO - 10.1093/mutage/geab012

M3 - Review

C2 - 33755160

VL - 36

SP - 193

EP - 212

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 3

ER -

ID: 276944584