Genomic analysis suggests higher susceptibility of children to air pollution
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Genomic analysis suggests higher susceptibility of children to air pollution. / van Leeuwen, Danitsja M; Pedersen, Marie; Hendriksen, Peter J M; Boorsma, André; van Herwijnen, Marcel H M; Gottschalk, Ralph W H; Kirsch-Volders, Micheline; Knudsen, Lisbeth E.; Srám, Radim J; Bajak, Edyta; van Delft, Joost H M; Kleinjans, Jos C S.
In: Carcinogenesis, Vol. 29, No. 5, 2008, p. 977-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genomic analysis suggests higher susceptibility of children to air pollution
AU - van Leeuwen, Danitsja M
AU - Pedersen, Marie
AU - Hendriksen, Peter J M
AU - Boorsma, André
AU - van Herwijnen, Marcel H M
AU - Gottschalk, Ralph W H
AU - Kirsch-Volders, Micheline
AU - Knudsen, Lisbeth E.
AU - Srám, Radim J
AU - Bajak, Edyta
AU - van Delft, Joost H M
AU - Kleinjans, Jos C S
PY - 2008
Y1 - 2008
N2 - Differences in biological responses to exposure to hazardous airborne substances between children and adults have been reported, suggesting children to be more susceptible. Aim of this study was to improve our understanding of differences in susceptibility in cancer risk associated with air pollution by comparing genome-wide gene expression profiles in peripheral blood of children and their parents. Gene expression analysis was performed in blood from children and parents living in two different regions in the Czech Republic with different levels of air pollution. Data were analyzed by two different approaches: one method first selected significantly differentially expressed genes and analyzed these gene lists for overrepresented biological processes, whereas the other applied the T-profiler tool to directly perform pathway analyses on the total gene set without preselection of significantly modulated gene expressions. In addition, gene expressions in both children and adults were investigated for associations with micronuclei frequencies. Both analysis approaches returned considerably more genes or gene groups and pathways that significantly differed between children from both regions than between parents. Very little overlap was observed between children and adults. The two most important biological processes or molecular functions significantly modulated in children, but not in adults, are nucleosome and immune response related. Our study suggests differences between children and adults in relation to air pollution exposure at the transcriptome level. The findings underline the necessity of implementing environmental health policy measures specifically for protecting children's health.
AB - Differences in biological responses to exposure to hazardous airborne substances between children and adults have been reported, suggesting children to be more susceptible. Aim of this study was to improve our understanding of differences in susceptibility in cancer risk associated with air pollution by comparing genome-wide gene expression profiles in peripheral blood of children and their parents. Gene expression analysis was performed in blood from children and parents living in two different regions in the Czech Republic with different levels of air pollution. Data were analyzed by two different approaches: one method first selected significantly differentially expressed genes and analyzed these gene lists for overrepresented biological processes, whereas the other applied the T-profiler tool to directly perform pathway analyses on the total gene set without preselection of significantly modulated gene expressions. In addition, gene expressions in both children and adults were investigated for associations with micronuclei frequencies. Both analysis approaches returned considerably more genes or gene groups and pathways that significantly differed between children from both regions than between parents. Very little overlap was observed between children and adults. The two most important biological processes or molecular functions significantly modulated in children, but not in adults, are nucleosome and immune response related. Our study suggests differences between children and adults in relation to air pollution exposure at the transcriptome level. The findings underline the necessity of implementing environmental health policy measures specifically for protecting children's health.
KW - Adult
KW - Air Pollution
KW - Child
KW - Czech Republic
KW - Female
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Genetic Predisposition to Disease
KW - Humans
KW - Male
KW - Nuclear Family
KW - Parents
KW - RNA
KW - RNA Splicing
KW - Receptors, Chemokine
U2 - 10.1093/carcin/bgn065
DO - 10.1093/carcin/bgn065
M3 - Journal article
C2 - 18332047
VL - 29
SP - 977
EP - 983
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 5
ER -
ID: 137758206