Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine. / Møller, Peter; Cooke, Marcus S; Collins, Andrew; Olinski, Ryszard; Rozalski, Rafal; Loft, Steffen.

In: Free Radical Research, Vol. 46, No. 4, 2012, p. 541-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Møller, P, Cooke, MS, Collins, A, Olinski, R, Rozalski, R & Loft, S 2012, 'Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine', Free Radical Research, vol. 46, no. 4, pp. 541-53. https://doi.org/10.3109/10715762.2011.644241

APA

Møller, P., Cooke, M. S., Collins, A., Olinski, R., Rozalski, R., & Loft, S. (2012). Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine. Free Radical Research, 46(4), 541-53. https://doi.org/10.3109/10715762.2011.644241

Vancouver

Møller P, Cooke MS, Collins A, Olinski R, Rozalski R, Loft S. Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine. Free Radical Research. 2012;46(4):541-53. https://doi.org/10.3109/10715762.2011.644241

Author

Møller, Peter ; Cooke, Marcus S ; Collins, Andrew ; Olinski, Ryszard ; Rozalski, Rafal ; Loft, Steffen. / Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine. In: Free Radical Research. 2012 ; Vol. 46, No. 4. pp. 541-53.

Bibtex

@article{8825ebb2daa540e7b95d516f9cf7f8c8,
title = "Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine",
abstract = "Levels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.",
author = "Peter M{\o}ller and Cooke, {Marcus S} and Andrew Collins and Ryszard Olinski and Rafal Rozalski and Steffen Loft",
year = "2012",
doi = "10.3109/10715762.2011.644241",
language = "English",
volume = "46",
pages = "541--53",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Taylor & Francis",
number = "4",

}

RIS

TY - JOUR

T1 - Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine

AU - Møller, Peter

AU - Cooke, Marcus S

AU - Collins, Andrew

AU - Olinski, Ryszard

AU - Rozalski, Rafal

AU - Loft, Steffen

PY - 2012

Y1 - 2012

N2 - Levels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.

AB - Levels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.

U2 - 10.3109/10715762.2011.644241

DO - 10.3109/10715762.2011.644241

M3 - Journal article

C2 - 22117555

VL - 46

SP - 541

EP - 553

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 4

ER -

ID: 38170853