High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. / Ipsen, David Højland; Tveden-Nyborg, Pernille; Rolin, Bidda; Rakipovski, Günaj; Beck, Maria; Mortensen, Line Winther; Færk, Lasse; Heegaard, Peter Mikael Helweg; Møller, Peter; Lykkesfeldt, Jens.

In: Nutrition & Metabolism, Vol. 13, 2016, p. 51.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ipsen, DH, Tveden-Nyborg, P, Rolin, B, Rakipovski, G, Beck, M, Mortensen, LW, Færk, L, Heegaard, PMH, Møller, P & Lykkesfeldt, J 2016, 'High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs', Nutrition & Metabolism, vol. 13, pp. 51. https://doi.org/10.1186/s12986-016-0110-1

APA

Ipsen, D. H., Tveden-Nyborg, P., Rolin, B., Rakipovski, G., Beck, M., Mortensen, L. W., ... Lykkesfeldt, J. (2016). High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. Nutrition & Metabolism, 13, 51. https://doi.org/10.1186/s12986-016-0110-1

Vancouver

Ipsen DH, Tveden-Nyborg P, Rolin B, Rakipovski G, Beck M, Mortensen LW et al. High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. Nutrition & Metabolism. 2016;13:51. https://doi.org/10.1186/s12986-016-0110-1

Author

Ipsen, David Højland ; Tveden-Nyborg, Pernille ; Rolin, Bidda ; Rakipovski, Günaj ; Beck, Maria ; Mortensen, Line Winther ; Færk, Lasse ; Heegaard, Peter Mikael Helweg ; Møller, Peter ; Lykkesfeldt, Jens. / High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. In: Nutrition & Metabolism. 2016 ; Vol. 13. pp. 51.

Bibtex

@article{4e7537cd8d5e4583b5c5dcfeb0788eca,
title = "High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs",
abstract = "BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) and dyslipidemia are closely related. Diet plays an important role in the progression of these diseases, but the role of specific dietary components is not completely understood. Therefore, we investigated the role of dietary sucrose and fat/cholesterol on the development of dyslipidemia and NAFLD.METHODS: Seventy female guinea pigs were block-randomized (based on weight) into five groups and fed a normal chow diet (control: 4 {\%} fat), a very high-sucrose diet (vHS: 4 {\%} fat, 25 {\%} sucrose), a high-fat diet (HF: 20 {\%} fat, 0.35 {\%} cholesterol), a high-fat/high-sucrose diet (HFHS: 20 {\%} fat, 15 {\%} sucrose, 0.35 {\%} cholesterol) or a high-fat/very high-sucrose diet (HFvHS: 20 {\%} fat, 25 {\%} sucrose, 0.35 {\%} cholesterol) for 16 and 25 weeks.RESULTS: All three high-fat diets induced dyslipidemia with increased concentrations of plasma cholesterol (p < 0.0001), LDL-C (p < 0.0001) and VLDL-C (p < 0.05) compared to control and vHS. Contrary to this, plasma triglycerides were increased in control and vHS compared to high-fat fed animals (p < 0.01), while circulating levels of free fatty acids were even between groups. Histological evaluation of liver sections revealed non-alcoholic steatohepatitis (NASH) with progressive inflammation and bridging fibrosis in high-fat fed animals. Accordingly, hepatic triglycerides (p < 0.05) and cholesterol (p < 0.0001) was increased alongside elevated levels of alanine and aspartate aminotransferase (p < 0.01) compared to control and vHS.CONCLUSION: Collectively, our results suggest that intake of fat and cholesterol, but not sucrose, are the main factors driving the development and progression of dyslipidemia and NAFLD/NASH.",
author = "Ipsen, {David H{\o}jland} and Pernille Tveden-Nyborg and Bidda Rolin and G{\"u}naj Rakipovski and Maria Beck and Mortensen, {Line Winther} and Lasse F{\ae}rk and Heegaard, {Peter Mikael Helweg} and Peter M{\o}ller and Jens Lykkesfeldt",
year = "2016",
doi = "10.1186/s12986-016-0110-1",
language = "English",
volume = "13",
pages = "51",
journal = "Nutrition & Metabolism",
issn = "1743-7075",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs

AU - Ipsen, David Højland

AU - Tveden-Nyborg, Pernille

AU - Rolin, Bidda

AU - Rakipovski, Günaj

AU - Beck, Maria

AU - Mortensen, Line Winther

AU - Færk, Lasse

AU - Heegaard, Peter Mikael Helweg

AU - Møller, Peter

AU - Lykkesfeldt, Jens

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) and dyslipidemia are closely related. Diet plays an important role in the progression of these diseases, but the role of specific dietary components is not completely understood. Therefore, we investigated the role of dietary sucrose and fat/cholesterol on the development of dyslipidemia and NAFLD.METHODS: Seventy female guinea pigs were block-randomized (based on weight) into five groups and fed a normal chow diet (control: 4 % fat), a very high-sucrose diet (vHS: 4 % fat, 25 % sucrose), a high-fat diet (HF: 20 % fat, 0.35 % cholesterol), a high-fat/high-sucrose diet (HFHS: 20 % fat, 15 % sucrose, 0.35 % cholesterol) or a high-fat/very high-sucrose diet (HFvHS: 20 % fat, 25 % sucrose, 0.35 % cholesterol) for 16 and 25 weeks.RESULTS: All three high-fat diets induced dyslipidemia with increased concentrations of plasma cholesterol (p < 0.0001), LDL-C (p < 0.0001) and VLDL-C (p < 0.05) compared to control and vHS. Contrary to this, plasma triglycerides were increased in control and vHS compared to high-fat fed animals (p < 0.01), while circulating levels of free fatty acids were even between groups. Histological evaluation of liver sections revealed non-alcoholic steatohepatitis (NASH) with progressive inflammation and bridging fibrosis in high-fat fed animals. Accordingly, hepatic triglycerides (p < 0.05) and cholesterol (p < 0.0001) was increased alongside elevated levels of alanine and aspartate aminotransferase (p < 0.01) compared to control and vHS.CONCLUSION: Collectively, our results suggest that intake of fat and cholesterol, but not sucrose, are the main factors driving the development and progression of dyslipidemia and NAFLD/NASH.

AB - BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) and dyslipidemia are closely related. Diet plays an important role in the progression of these diseases, but the role of specific dietary components is not completely understood. Therefore, we investigated the role of dietary sucrose and fat/cholesterol on the development of dyslipidemia and NAFLD.METHODS: Seventy female guinea pigs were block-randomized (based on weight) into five groups and fed a normal chow diet (control: 4 % fat), a very high-sucrose diet (vHS: 4 % fat, 25 % sucrose), a high-fat diet (HF: 20 % fat, 0.35 % cholesterol), a high-fat/high-sucrose diet (HFHS: 20 % fat, 15 % sucrose, 0.35 % cholesterol) or a high-fat/very high-sucrose diet (HFvHS: 20 % fat, 25 % sucrose, 0.35 % cholesterol) for 16 and 25 weeks.RESULTS: All three high-fat diets induced dyslipidemia with increased concentrations of plasma cholesterol (p < 0.0001), LDL-C (p < 0.0001) and VLDL-C (p < 0.05) compared to control and vHS. Contrary to this, plasma triglycerides were increased in control and vHS compared to high-fat fed animals (p < 0.01), while circulating levels of free fatty acids were even between groups. Histological evaluation of liver sections revealed non-alcoholic steatohepatitis (NASH) with progressive inflammation and bridging fibrosis in high-fat fed animals. Accordingly, hepatic triglycerides (p < 0.05) and cholesterol (p < 0.0001) was increased alongside elevated levels of alanine and aspartate aminotransferase (p < 0.01) compared to control and vHS.CONCLUSION: Collectively, our results suggest that intake of fat and cholesterol, but not sucrose, are the main factors driving the development and progression of dyslipidemia and NAFLD/NASH.

U2 - 10.1186/s12986-016-0110-1

DO - 10.1186/s12986-016-0110-1

M3 - Journal article

VL - 13

SP - 51

JO - Nutrition & Metabolism

JF - Nutrition & Metabolism

SN - 1743-7075

ER -

ID: 165568292