High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals. / Norskov, M.S.; Frikke-Schmidt, R.; Loft, S.; Tybjaerg-Hansen, A.

In: Clinical Biochemistry, Vol. 42, No. 3, 2009, p. 201-209.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Norskov, MS, Frikke-Schmidt, R, Loft, S & Tybjaerg-Hansen, A 2009, 'High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals', Clinical Biochemistry, vol. 42, no. 3, pp. 201-209.

APA

Norskov, M. S., Frikke-Schmidt, R., Loft, S., & Tybjaerg-Hansen, A. (2009). High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals. Clinical Biochemistry, 42(3), 201-209.

Vancouver

Norskov MS, Frikke-Schmidt R, Loft S, Tybjaerg-Hansen A. High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals. Clinical Biochemistry. 2009;42(3):201-209.

Author

Norskov, M.S. ; Frikke-Schmidt, R. ; Loft, S. ; Tybjaerg-Hansen, A. / High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals. In: Clinical Biochemistry. 2009 ; Vol. 42, No. 3. pp. 201-209.

Bibtex

@article{db347940631011df928f000ea68e967b,
title = "High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals",
abstract = "OBJECTIVES: Characteristic for the genes encoding glutathione S-transferase (GST) M1 and GSTT1 is a null allele, suggested to increase susceptibility to chronic diseases. We report an optimized method for the determination of copy number variation (CNV) in GST genes. DESIGN AND METHODS: Real-time multiplex PCR reactions were optimized for quantification of GSTM1 and GSTT1 CNV using the DeltaCt method, a fixed volume of diluted DNA, a total volume of 10 microL, 384-well formats, and single determinations of each sample. RESULTS: Consistent genotyping was obtained using DNA in a range of 0.41 ng to 100 ng. In a general population sample of 20,687 individuals the genotype frequencies were concordant with other methods used as standards. Throughput was 4600 genotypes per day at a reagent price of 0.5 euros per sample. CONCLUSIONS: This high-throughput, low cost method accurately determines CNV in the GST genes enabling reliable estimates of disease prediction in large epidemiological samples Udgivelsesdato: 2009/2",
author = "M.S. Norskov and R. Frikke-Schmidt and S. Loft and A. Tybjaerg-Hansen",
year = "2009",
language = "English",
volume = "42",
pages = "201--209",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - High-throughput genotyping of copy number variation in glutathione S-transferases M1 and T1 using real-time PCR in 20,687 individuals

AU - Norskov, M.S.

AU - Frikke-Schmidt, R.

AU - Loft, S.

AU - Tybjaerg-Hansen, A.

PY - 2009

Y1 - 2009

N2 - OBJECTIVES: Characteristic for the genes encoding glutathione S-transferase (GST) M1 and GSTT1 is a null allele, suggested to increase susceptibility to chronic diseases. We report an optimized method for the determination of copy number variation (CNV) in GST genes. DESIGN AND METHODS: Real-time multiplex PCR reactions were optimized for quantification of GSTM1 and GSTT1 CNV using the DeltaCt method, a fixed volume of diluted DNA, a total volume of 10 microL, 384-well formats, and single determinations of each sample. RESULTS: Consistent genotyping was obtained using DNA in a range of 0.41 ng to 100 ng. In a general population sample of 20,687 individuals the genotype frequencies were concordant with other methods used as standards. Throughput was 4600 genotypes per day at a reagent price of 0.5 euros per sample. CONCLUSIONS: This high-throughput, low cost method accurately determines CNV in the GST genes enabling reliable estimates of disease prediction in large epidemiological samples Udgivelsesdato: 2009/2

AB - OBJECTIVES: Characteristic for the genes encoding glutathione S-transferase (GST) M1 and GSTT1 is a null allele, suggested to increase susceptibility to chronic diseases. We report an optimized method for the determination of copy number variation (CNV) in GST genes. DESIGN AND METHODS: Real-time multiplex PCR reactions were optimized for quantification of GSTM1 and GSTT1 CNV using the DeltaCt method, a fixed volume of diluted DNA, a total volume of 10 microL, 384-well formats, and single determinations of each sample. RESULTS: Consistent genotyping was obtained using DNA in a range of 0.41 ng to 100 ng. In a general population sample of 20,687 individuals the genotype frequencies were concordant with other methods used as standards. Throughput was 4600 genotypes per day at a reagent price of 0.5 euros per sample. CONCLUSIONS: This high-throughput, low cost method accurately determines CNV in the GST genes enabling reliable estimates of disease prediction in large epidemiological samples Udgivelsesdato: 2009/2

M3 - Journal article

VL - 42

SP - 201

EP - 209

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 3

ER -

ID: 19819399