Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. / Barregard, Lars; Møller, Peter; Henriksen, Trine; Mistry, Vilas; Koppen, Gudrun; Rossner, Pavel; Sram, Radim J; Weimann, Allan; Poulsen, Henrik E; Nataf, Robert; Andreoli, Roberta; Manini, Paola; Marczylo, Tim; Lam, Patricia; Evans, Mark D; Kasai, Hiroshi; Kawai, Kazuaki; Li, Yun-Shan; Sakai, Kazuo; Singh, Rajinder; Teichert, Friederike; Farmer, Peter B; Rozalski, Rafal; Gackowski, Daniel; Siomek, Agnieszka; Saez, Guillermo T; Cerda, Concha; Broberg, Karin; Lindh, Christian; Hossain, Mohammad Bakhtiar; Haghdoost, Siamak; Hu, Chiung-Wen; Chao, Mu-Rong; Wu, Kuen-Yuh; Orhan, Hilmi; Senduran, Nilufer; Smith, Raymond J; Santella, Regina M; Su, Yali; Cortez, Czarina; Yeh, Susan; Olinski, Ryszard; Loft, Steffen; Cooke, Marcus S.

In: Antioxidants & Redox Signaling, Vol. 18, No. 18, 20.06.2013, p. 2377-91.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Barregard, L, Møller, P, Henriksen, T, Mistry, V, Koppen, G, Rossner, P, Sram, RJ, Weimann, A, Poulsen, HE, Nataf, R, Andreoli, R, Manini, P, Marczylo, T, Lam, P, Evans, MD, Kasai, H, Kawai, K, Li, Y-S, Sakai, K, Singh, R, Teichert, F, Farmer, PB, Rozalski, R, Gackowski, D, Siomek, A, Saez, GT, Cerda, C, Broberg, K, Lindh, C, Hossain, MB, Haghdoost, S, Hu, C-W, Chao, M-R, Wu, K-Y, Orhan, H, Senduran, N, Smith, RJ, Santella, RM, Su, Y, Cortez, C, Yeh, S, Olinski, R, Loft, S & Cooke, MS 2013, 'Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine', Antioxidants & Redox Signaling, vol. 18, no. 18, pp. 2377-91. https://doi.org/10.1089/ars.2012.4714

APA

Barregard, L., Møller, P., Henriksen, T., Mistry, V., Koppen, G., Rossner, P., Sram, R. J., Weimann, A., Poulsen, H. E., Nataf, R., Andreoli, R., Manini, P., Marczylo, T., Lam, P., Evans, M. D., Kasai, H., Kawai, K., Li, Y-S., Sakai, K., ... Cooke, M. S. (2013). Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. Antioxidants & Redox Signaling, 18(18), 2377-91. https://doi.org/10.1089/ars.2012.4714

Vancouver

Barregard L, Møller P, Henriksen T, Mistry V, Koppen G, Rossner P et al. Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. Antioxidants & Redox Signaling. 2013 Jun 20;18(18):2377-91. https://doi.org/10.1089/ars.2012.4714

Author

Barregard, Lars ; Møller, Peter ; Henriksen, Trine ; Mistry, Vilas ; Koppen, Gudrun ; Rossner, Pavel ; Sram, Radim J ; Weimann, Allan ; Poulsen, Henrik E ; Nataf, Robert ; Andreoli, Roberta ; Manini, Paola ; Marczylo, Tim ; Lam, Patricia ; Evans, Mark D ; Kasai, Hiroshi ; Kawai, Kazuaki ; Li, Yun-Shan ; Sakai, Kazuo ; Singh, Rajinder ; Teichert, Friederike ; Farmer, Peter B ; Rozalski, Rafal ; Gackowski, Daniel ; Siomek, Agnieszka ; Saez, Guillermo T ; Cerda, Concha ; Broberg, Karin ; Lindh, Christian ; Hossain, Mohammad Bakhtiar ; Haghdoost, Siamak ; Hu, Chiung-Wen ; Chao, Mu-Rong ; Wu, Kuen-Yuh ; Orhan, Hilmi ; Senduran, Nilufer ; Smith, Raymond J ; Santella, Regina M ; Su, Yali ; Cortez, Czarina ; Yeh, Susan ; Olinski, Ryszard ; Loft, Steffen ; Cooke, Marcus S. / Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. In: Antioxidants & Redox Signaling. 2013 ; Vol. 18, No. 18. pp. 2377-91.

Bibtex

@article{0b029ba66f6b4bcdaaf07500390025ff,
title = "Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine",
abstract = "Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.",
keywords = "Adult, Artifacts, Buffers, Deoxyguanosine, Female, Head and Neck Neoplasms, Humans, Male, Middle Aged, Reference Standards, Reproducibility of Results, Sodium Chloride, Solutions, Urinalysis, Young Adult",
author = "Lars Barregard and Peter M{\o}ller and Trine Henriksen and Vilas Mistry and Gudrun Koppen and Pavel Rossner and Sram, {Radim J} and Allan Weimann and Poulsen, {Henrik E} and Robert Nataf and Roberta Andreoli and Paola Manini and Tim Marczylo and Patricia Lam and Evans, {Mark D} and Hiroshi Kasai and Kazuaki Kawai and Yun-Shan Li and Kazuo Sakai and Rajinder Singh and Friederike Teichert and Farmer, {Peter B} and Rafal Rozalski and Daniel Gackowski and Agnieszka Siomek and Saez, {Guillermo T} and Concha Cerda and Karin Broberg and Christian Lindh and Hossain, {Mohammad Bakhtiar} and Siamak Haghdoost and Chiung-Wen Hu and Mu-Rong Chao and Kuen-Yuh Wu and Hilmi Orhan and Nilufer Senduran and Smith, {Raymond J} and Santella, {Regina M} and Yali Su and Czarina Cortez and Susan Yeh and Ryszard Olinski and Steffen Loft and Cooke, {Marcus S}",
year = "2013",
month = jun,
day = "20",
doi = "10.1089/ars.2012.4714",
language = "English",
volume = "18",
pages = "2377--91",
journal = "Antioxidants & Redox Signaling",
issn = "1523-0864",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "18",

}

RIS

TY - JOUR

T1 - Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

AU - Barregard, Lars

AU - Møller, Peter

AU - Henriksen, Trine

AU - Mistry, Vilas

AU - Koppen, Gudrun

AU - Rossner, Pavel

AU - Sram, Radim J

AU - Weimann, Allan

AU - Poulsen, Henrik E

AU - Nataf, Robert

AU - Andreoli, Roberta

AU - Manini, Paola

AU - Marczylo, Tim

AU - Lam, Patricia

AU - Evans, Mark D

AU - Kasai, Hiroshi

AU - Kawai, Kazuaki

AU - Li, Yun-Shan

AU - Sakai, Kazuo

AU - Singh, Rajinder

AU - Teichert, Friederike

AU - Farmer, Peter B

AU - Rozalski, Rafal

AU - Gackowski, Daniel

AU - Siomek, Agnieszka

AU - Saez, Guillermo T

AU - Cerda, Concha

AU - Broberg, Karin

AU - Lindh, Christian

AU - Hossain, Mohammad Bakhtiar

AU - Haghdoost, Siamak

AU - Hu, Chiung-Wen

AU - Chao, Mu-Rong

AU - Wu, Kuen-Yuh

AU - Orhan, Hilmi

AU - Senduran, Nilufer

AU - Smith, Raymond J

AU - Santella, Regina M

AU - Su, Yali

AU - Cortez, Czarina

AU - Yeh, Susan

AU - Olinski, Ryszard

AU - Loft, Steffen

AU - Cooke, Marcus S

PY - 2013/6/20

Y1 - 2013/6/20

N2 - Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.

AB - Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.

KW - Adult

KW - Artifacts

KW - Buffers

KW - Deoxyguanosine

KW - Female

KW - Head and Neck Neoplasms

KW - Humans

KW - Male

KW - Middle Aged

KW - Reference Standards

KW - Reproducibility of Results

KW - Sodium Chloride

KW - Solutions

KW - Urinalysis

KW - Young Adult

U2 - 10.1089/ars.2012.4714

DO - 10.1089/ars.2012.4714

M3 - Journal article

C2 - 23198723

VL - 18

SP - 2377

EP - 2391

JO - Antioxidants & Redox Signaling

JF - Antioxidants & Redox Signaling

SN - 1523-0864

IS - 18

ER -

ID: 98468072