Induction of DNA repair synthesis in human monocytes/B-lymphocytes compared with T-lymphocytes after exposure to N-acetoxy-N-acetylaminofluorene and dimethylsulfate in vitro

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Lisbeth E. Knudsen, L P Ryder, K Wassermann

We have explored the induction of DNA repair synthesis in monocyte/B- and T-lymphocyte enriched cell fractions from 12 different human mononuclear blood cell populations. Unscheduled DNA synthesis was measured in monocyte/B- and T-cells after exposure to the DNA-damaging agents dimethylsulfate (DMS) and N-acetoxy-N-acetylaminofluorene in vitro. Also, the binding of DMS to DNA was measured. An increased DNA repair synthesis was measured in monocyte/B-lymphocytes after induction of the two different types of DNA lesions, whereas no induction of unscheduled DNA synthesis was observed in T-lymphocytes. A significantly higher DMS-DNA binding was also observed in monocyte/B-lymphocytes when compared with T-lymphocytes. Specific characterization of mononuclear blood cell populations used in biomonitoring of DNA adducts and repair is recommended.

Original languageEnglish
JournalCarcinogenesis
Volume13
Issue number7
Pages (from-to)1285-7
Number of pages3
ISSN0143-3334
Publication statusPublished - Jul 1992

    Research areas

  • Acetoxyacetylaminofluorene, B-Lymphocytes, Cells, Cultured, DNA Repair, DNA Replication, Humans, Monocytes, Sulfuric Acid Esters, T-Lymphocytes

ID: 137758792