Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity. / Jensen, Annie; Løhr, Mille; Eriksen, Louise; Grønbæk, Morten; Dorry, Elad; Loft, Steffen; Møller, Peter.
In: Free Radical Biology & Medicine, Vol. 52, No. 1, 2012, p. 118-25.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity
AU - Jensen, Annie
AU - Løhr, Mille
AU - Eriksen, Louise
AU - Grønbæk, Morten
AU - Dorry, Elad
AU - Loft, Steffen
AU - Møller, Peter
N1 - Copyright © 2011 Elsevier Inc. All rights reserved.
PY - 2012
Y1 - 2012
N2 - Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P
AB - Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P
U2 - 10.1016/j.freeradbiomed.2011.09.038
DO - 10.1016/j.freeradbiomed.2011.09.038
M3 - Journal article
C2 - 22019439
VL - 52
SP - 118
EP - 125
JO - Free Radical Biology & Medicine
JF - Free Radical Biology & Medicine
SN - 0891-5849
IS - 1
ER -
ID: 37550900