Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats
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Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats. / Jørgensen, Anders; Breitenstein, Katrine; Kalliokoski, Otto; Weimann, Allan; Henriksen, Trine; Poulsen, Henrik Enghusen; Jørgensen, Martin Balslev; Wörtwein, Gitta.
In: Acta Neuropsychiatrica, Vol. 31, No. 6, 2019, p. 287-293.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats
AU - Jørgensen, Anders
AU - Breitenstein, Katrine
AU - Kalliokoski, Otto
AU - Weimann, Allan
AU - Henriksen, Trine
AU - Poulsen, Henrik Enghusen
AU - Jørgensen, Martin Balslev
AU - Wörtwein, Gitta
PY - 2019
Y1 - 2019
N2 - Objective:Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT); a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side-effects of ECT.Methods:We measured sensitive markers of systemic and CNS oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS); an animal model of ECT. Due to previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results:ECS induced weight loss, corticosterone increases (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion:These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young or middle-aged rats.
AB - Objective:Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT); a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side-effects of ECT.Methods:We measured sensitive markers of systemic and CNS oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS); an animal model of ECT. Due to previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results:ECS induced weight loss, corticosterone increases (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion:These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young or middle-aged rats.
KW - DNA repair
KW - Electroconvulsive stimulations
KW - HPA-axis
KW - nucleic acids
KW - oxidative stress
U2 - 10.1017/neu.2019.7
DO - 10.1017/neu.2019.7
M3 - Journal article
C2 - 30854991
AN - SCOPUS:85063165332
VL - 31
SP - 287
EP - 293
JO - Acta Neuropsychiatrica
JF - Acta Neuropsychiatrica
SN - 0924-2708
IS - 6
ER -
ID: 223975506