Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals

Research output: Contribution to journalReviewResearchpeer-review

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Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals. / Møller, Peter; Jensen, Ditte Marie; Christophersen, Daniel Vest; Kermanizadeh, Ali; Jacobsen, Nicklas Raun; Hemmingsen, Jette Gjerke; Danielsen, Pernille Høgh; Karottki, Dorina Gabriela; Roursgaard, Martin; Cao, Yi; Jantzen, Kim; Klingberg, Henrik; Hersoug, Lars-Georg; Loft, Steffen.

In: Environmental and Molecular Mutagenesis, Vol. 56, No. 2, 03.2015, p. 97-110.

Research output: Contribution to journalReviewResearchpeer-review

Harvard

Møller, P, Jensen, DM, Christophersen, DV, Kermanizadeh, A, Jacobsen, NR, Hemmingsen, JG, Danielsen, PH, Karottki, DG, Roursgaard, M, Cao, Y, Jantzen, K, Klingberg, H, Hersoug, L-G & Loft, S 2015, 'Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals', Environmental and Molecular Mutagenesis, vol. 56, no. 2, pp. 97-110. https://doi.org/10.1002/em.21899

APA

Møller, P., Jensen, D. M., Christophersen, D. V., Kermanizadeh, A., Jacobsen, N. R., Hemmingsen, J. G., ... Loft, S. (2015). Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals. Environmental and Molecular Mutagenesis, 56(2), 97-110. https://doi.org/10.1002/em.21899

Vancouver

Møller P, Jensen DM, Christophersen DV, Kermanizadeh A, Jacobsen NR, Hemmingsen JG et al. Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals. Environmental and Molecular Mutagenesis. 2015 Mar;56(2):97-110. https://doi.org/10.1002/em.21899

Author

Møller, Peter ; Jensen, Ditte Marie ; Christophersen, Daniel Vest ; Kermanizadeh, Ali ; Jacobsen, Nicklas Raun ; Hemmingsen, Jette Gjerke ; Danielsen, Pernille Høgh ; Karottki, Dorina Gabriela ; Roursgaard, Martin ; Cao, Yi ; Jantzen, Kim ; Klingberg, Henrik ; Hersoug, Lars-Georg ; Loft, Steffen. / Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals. In: Environmental and Molecular Mutagenesis. 2015 ; Vol. 56, No. 2. pp. 97-110.

Bibtex

@article{f18ae4eebc7a4ba79d2c7a4512412cc7,
title = "Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals",
abstract = "Increased levels of oxidatively damaged DNA have been documented in studies of metal, metal oxide, carbon-based and ceramic engineered nanomaterials (ENMs). In particular, 8-oxo-7,8-dihydroguanine-2'-deoxyguanosine (8-oxodG) is widely assessed as a DNA nucleobase oxidation product, measured by chromatographic assays, antibody-based methods or the comet assay with DNA repair enzymes. However, spurious oxidation of DNA has been a problem in certain studies applying chromatographic assays, yielding high baseline levels of 8-oxodG. Antibody-based assays detect high 8-oxodG baseline levels, related to cross-reactivity with other molecules in cells. This review provides an overview of efforts to reliably detect oxidatively damaged DNA and a critical assessment of the published studies on DNA damage levels. Animal studies with high baseline levels of oxidatively damaged DNA are more likely to show positive associations between airway exposure to ENMs and oxidized DNA in lung tissue than studies showing acceptable baseline levels (odds ratio = 12.1, 95{\%} confidence interval: 1.2-124). Nevertheless, reliable studies indicate that intratracheal instillation of nanosized carbon black is associated with increased levels of oxidatively damaged DNA in lung tissue. Oral exposure to nanosized carbon black, TiO2 , carbon nanotubes and ZnO is associated with elevated levels of oxidatively damaged DNA in tissues. These observations are supported by cell culture studies showing concentration-dependent associations between ENM exposure and oxidatively damaged DNA measured by the comet assay. Cell culture studies show relatively high variation in the ability of ENMs to oxidatively damage DNA; hence, it is currently impossible to group ENMs according to their DNA damaging potential. Environ. Mol. Mutagen., 2014. {\circledC} 2014 Wiley Periodicals, Inc.",
author = "Peter M{\o}ller and Jensen, {Ditte Marie} and Christophersen, {Daniel Vest} and Ali Kermanizadeh and Jacobsen, {Nicklas Raun} and Hemmingsen, {Jette Gjerke} and Danielsen, {Pernille H{\o}gh} and Karottki, {Dorina Gabriela} and Martin Roursgaard and Yi Cao and Kim Jantzen and Henrik Klingberg and Lars-Georg Hersoug and Steffen Loft",
note = "Copyright {\circledC} 2014 Wiley Periodicals, Inc.",
year = "2015",
month = "3",
doi = "10.1002/em.21899",
language = "English",
volume = "56",
pages = "97--110",
journal = "Environmental and Molecular Mutagenesis",
issn = "0893-6692",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Measurement of oxidative damage to DNA in nanomaterial exposed cells and animals

AU - Møller, Peter

AU - Jensen, Ditte Marie

AU - Christophersen, Daniel Vest

AU - Kermanizadeh, Ali

AU - Jacobsen, Nicklas Raun

AU - Hemmingsen, Jette Gjerke

AU - Danielsen, Pernille Høgh

AU - Karottki, Dorina Gabriela

AU - Roursgaard, Martin

AU - Cao, Yi

AU - Jantzen, Kim

AU - Klingberg, Henrik

AU - Hersoug, Lars-Georg

AU - Loft, Steffen

N1 - Copyright © 2014 Wiley Periodicals, Inc.

PY - 2015/3

Y1 - 2015/3

N2 - Increased levels of oxidatively damaged DNA have been documented in studies of metal, metal oxide, carbon-based and ceramic engineered nanomaterials (ENMs). In particular, 8-oxo-7,8-dihydroguanine-2'-deoxyguanosine (8-oxodG) is widely assessed as a DNA nucleobase oxidation product, measured by chromatographic assays, antibody-based methods or the comet assay with DNA repair enzymes. However, spurious oxidation of DNA has been a problem in certain studies applying chromatographic assays, yielding high baseline levels of 8-oxodG. Antibody-based assays detect high 8-oxodG baseline levels, related to cross-reactivity with other molecules in cells. This review provides an overview of efforts to reliably detect oxidatively damaged DNA and a critical assessment of the published studies on DNA damage levels. Animal studies with high baseline levels of oxidatively damaged DNA are more likely to show positive associations between airway exposure to ENMs and oxidized DNA in lung tissue than studies showing acceptable baseline levels (odds ratio = 12.1, 95% confidence interval: 1.2-124). Nevertheless, reliable studies indicate that intratracheal instillation of nanosized carbon black is associated with increased levels of oxidatively damaged DNA in lung tissue. Oral exposure to nanosized carbon black, TiO2 , carbon nanotubes and ZnO is associated with elevated levels of oxidatively damaged DNA in tissues. These observations are supported by cell culture studies showing concentration-dependent associations between ENM exposure and oxidatively damaged DNA measured by the comet assay. Cell culture studies show relatively high variation in the ability of ENMs to oxidatively damage DNA; hence, it is currently impossible to group ENMs according to their DNA damaging potential. Environ. Mol. Mutagen., 2014. © 2014 Wiley Periodicals, Inc.

AB - Increased levels of oxidatively damaged DNA have been documented in studies of metal, metal oxide, carbon-based and ceramic engineered nanomaterials (ENMs). In particular, 8-oxo-7,8-dihydroguanine-2'-deoxyguanosine (8-oxodG) is widely assessed as a DNA nucleobase oxidation product, measured by chromatographic assays, antibody-based methods or the comet assay with DNA repair enzymes. However, spurious oxidation of DNA has been a problem in certain studies applying chromatographic assays, yielding high baseline levels of 8-oxodG. Antibody-based assays detect high 8-oxodG baseline levels, related to cross-reactivity with other molecules in cells. This review provides an overview of efforts to reliably detect oxidatively damaged DNA and a critical assessment of the published studies on DNA damage levels. Animal studies with high baseline levels of oxidatively damaged DNA are more likely to show positive associations between airway exposure to ENMs and oxidized DNA in lung tissue than studies showing acceptable baseline levels (odds ratio = 12.1, 95% confidence interval: 1.2-124). Nevertheless, reliable studies indicate that intratracheal instillation of nanosized carbon black is associated with increased levels of oxidatively damaged DNA in lung tissue. Oral exposure to nanosized carbon black, TiO2 , carbon nanotubes and ZnO is associated with elevated levels of oxidatively damaged DNA in tissues. These observations are supported by cell culture studies showing concentration-dependent associations between ENM exposure and oxidatively damaged DNA measured by the comet assay. Cell culture studies show relatively high variation in the ability of ENMs to oxidatively damage DNA; hence, it is currently impossible to group ENMs according to their DNA damaging potential. Environ. Mol. Mutagen., 2014. © 2014 Wiley Periodicals, Inc.

U2 - 10.1002/em.21899

DO - 10.1002/em.21899

M3 - Review

C2 - 25196723

VL - 56

SP - 97

EP - 110

JO - Environmental and Molecular Mutagenesis

JF - Environmental and Molecular Mutagenesis

SN - 0893-6692

IS - 2

ER -

ID: 162898235