Microhabitat preference of Anisakis simplex in three salmonid species: immunological implications

Research output: Contribution to journalJournal articlepeer-review

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Microhabitat preference of Anisakis simplex in three salmonid species : immunological implications. / Bahlool, Qusay Zuhair Mohammad; Skovgaard, Alf; Kania, Per Walter; Haarder, Simon; Buchmann, Kurt.

In: Veterinary Parasitology, Vol. 190, No. 3-4, 2012, p. 489-495.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bahlool, QZM, Skovgaard, A, Kania, PW, Haarder, S & Buchmann, K 2012, 'Microhabitat preference of Anisakis simplex in three salmonid species: immunological implications', Veterinary Parasitology, vol. 190, no. 3-4, pp. 489-495. https://doi.org/10.1016/j.vetpar.2012.07.009

APA

Bahlool, Q. Z. M., Skovgaard, A., Kania, P. W., Haarder, S., & Buchmann, K. (2012). Microhabitat preference of Anisakis simplex in three salmonid species: immunological implications. Veterinary Parasitology, 190(3-4), 489-495. https://doi.org/10.1016/j.vetpar.2012.07.009

Vancouver

Bahlool QZM, Skovgaard A, Kania PW, Haarder S, Buchmann K. Microhabitat preference of Anisakis simplex in three salmonid species: immunological implications. Veterinary Parasitology. 2012;190(3-4):489-495. https://doi.org/10.1016/j.vetpar.2012.07.009

Author

Bahlool, Qusay Zuhair Mohammad ; Skovgaard, Alf ; Kania, Per Walter ; Haarder, Simon ; Buchmann, Kurt. / Microhabitat preference of Anisakis simplex in three salmonid species : immunological implications. In: Veterinary Parasitology. 2012 ; Vol. 190, No. 3-4. pp. 489-495.

Bibtex

@article{d1ad7b7328b84e788776ea8f9eaf5e12,
title = "Microhabitat preference of Anisakis simplex in three salmonid species: immunological implications",
abstract = "Three salmonid fish species, Oncorhynchus mykiss, Salmo salar and Salmo trutta, were infected experimentally with the parasitic nematode Anisakis simplex (A. simplex) and the difference between in vivo behaviour of the nematode in the three fish species was investigated. Infection success rate differed between species. S. salar (Baltic salmon) showed the highest number of successfully established nematodes, whereas S. trutta (brown trout) and O. mykiss (rainbow trout) had a higher natural resistance. Microhabitat selection of nematodes differed according to fish species. In brown trout, A. simplex larvae were attached to the digestive tract (stomach, pyloric caeca, intestine), while the majority of larvae found in rainbow trout were located between the pyloric caeca. In Baltic salmon, nematodes were dispersed in and on spleen, head kidney, liver, swim bladder and musculature. Encapsulation and inflammatory cellular reactions differed accordingly. Histopathological and immunohistochemical studies using monoclonal antibodies raised against salmonid IgM, CD8 and MHCII were performed to detect the presence of immune cells around the infecting nematodes. None of the three fish species showed positive reactions for IgM-bearing cells in the inflammatory tissue connected with nematodes. CD8+ cells were detected in all three species and MHCII-bearing cells were found associated with encapsulated A. simplex in rainbow trout and brown trout, but not in Baltic salmon. Physiological, immunological and pathological implications of microhabitat differences are discussed.",
keywords = "Natural medicine, Anisakis simplex, Fish immunology, Immunohistochemistry, Anisakis simplex , Fish immunology, Immunohistochemistry",
author = "Bahlool, {Qusay Zuhair Mohammad} and Alf Skovgaard and Kania, {Per Walter} and Simon Haarder and Kurt Buchmann",
year = "2012",
doi = "10.1016/j.vetpar.2012.07.009",
language = "English",
volume = "190",
pages = "489--495",
journal = "Veterinary Parasitology",
issn = "0304-4017",
publisher = "Elsevier",
number = "3-4",

}

RIS

TY - JOUR

T1 - Microhabitat preference of Anisakis simplex in three salmonid species

T2 - immunological implications

AU - Bahlool, Qusay Zuhair Mohammad

AU - Skovgaard, Alf

AU - Kania, Per Walter

AU - Haarder, Simon

AU - Buchmann, Kurt

PY - 2012

Y1 - 2012

N2 - Three salmonid fish species, Oncorhynchus mykiss, Salmo salar and Salmo trutta, were infected experimentally with the parasitic nematode Anisakis simplex (A. simplex) and the difference between in vivo behaviour of the nematode in the three fish species was investigated. Infection success rate differed between species. S. salar (Baltic salmon) showed the highest number of successfully established nematodes, whereas S. trutta (brown trout) and O. mykiss (rainbow trout) had a higher natural resistance. Microhabitat selection of nematodes differed according to fish species. In brown trout, A. simplex larvae were attached to the digestive tract (stomach, pyloric caeca, intestine), while the majority of larvae found in rainbow trout were located between the pyloric caeca. In Baltic salmon, nematodes were dispersed in and on spleen, head kidney, liver, swim bladder and musculature. Encapsulation and inflammatory cellular reactions differed accordingly. Histopathological and immunohistochemical studies using monoclonal antibodies raised against salmonid IgM, CD8 and MHCII were performed to detect the presence of immune cells around the infecting nematodes. None of the three fish species showed positive reactions for IgM-bearing cells in the inflammatory tissue connected with nematodes. CD8+ cells were detected in all three species and MHCII-bearing cells were found associated with encapsulated A. simplex in rainbow trout and brown trout, but not in Baltic salmon. Physiological, immunological and pathological implications of microhabitat differences are discussed.

AB - Three salmonid fish species, Oncorhynchus mykiss, Salmo salar and Salmo trutta, were infected experimentally with the parasitic nematode Anisakis simplex (A. simplex) and the difference between in vivo behaviour of the nematode in the three fish species was investigated. Infection success rate differed between species. S. salar (Baltic salmon) showed the highest number of successfully established nematodes, whereas S. trutta (brown trout) and O. mykiss (rainbow trout) had a higher natural resistance. Microhabitat selection of nematodes differed according to fish species. In brown trout, A. simplex larvae were attached to the digestive tract (stomach, pyloric caeca, intestine), while the majority of larvae found in rainbow trout were located between the pyloric caeca. In Baltic salmon, nematodes were dispersed in and on spleen, head kidney, liver, swim bladder and musculature. Encapsulation and inflammatory cellular reactions differed accordingly. Histopathological and immunohistochemical studies using monoclonal antibodies raised against salmonid IgM, CD8 and MHCII were performed to detect the presence of immune cells around the infecting nematodes. None of the three fish species showed positive reactions for IgM-bearing cells in the inflammatory tissue connected with nematodes. CD8+ cells were detected in all three species and MHCII-bearing cells were found associated with encapsulated A. simplex in rainbow trout and brown trout, but not in Baltic salmon. Physiological, immunological and pathological implications of microhabitat differences are discussed.

KW - Natural medicine

KW - Anisakis simplex

KW - Fish immunology

KW - Immunohistochemistry

KW - Anisakis simplex

KW - Fish immunology

KW - Immunohistochemistry

U2 - 10.1016/j.vetpar.2012.07.009

DO - 10.1016/j.vetpar.2012.07.009

M3 - Journal article

C2 - 22858638

VL - 190

SP - 489

EP - 495

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

IS - 3-4

ER -

ID: 40246984