Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis

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Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis. / Vesterdal, Lise K; Folkmann, Janne K; Jacobsen, Nicklas R; Sheykhzade, Majid; Wallin, Håkan; Loft, Steffen; Møller, Peter.

In: Particle and Fibre Toxicology, Vol. 6, 2009, p. 5.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Vesterdal, LK, Folkmann, JK, Jacobsen, NR, Sheykhzade, M, Wallin, H, Loft, S & Møller, P 2009, 'Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis', Particle and Fibre Toxicology, vol. 6, pp. 5. https://doi.org/10.1186/1743-8977-6-5

APA

Vesterdal, L. K., Folkmann, J. K., Jacobsen, N. R., Sheykhzade, M., Wallin, H., Loft, S., & Møller, P. (2009). Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis. Particle and Fibre Toxicology, 6, 5. https://doi.org/10.1186/1743-8977-6-5

Vancouver

Vesterdal LK, Folkmann JK, Jacobsen NR, Sheykhzade M, Wallin H, Loft S et al. Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis. Particle and Fibre Toxicology. 2009;6:5. https://doi.org/10.1186/1743-8977-6-5

Author

Vesterdal, Lise K ; Folkmann, Janne K ; Jacobsen, Nicklas R ; Sheykhzade, Majid ; Wallin, Håkan ; Loft, Steffen ; Møller, Peter. / Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis. In: Particle and Fibre Toxicology. 2009 ; Vol. 6. pp. 5.

Bibtex

@article{b5afbfb05b0d11dea8de000ea68e967b,
title = "Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis",
abstract = "ABSTRACT: BACKGROUND: Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C60 fullerenes are nano-sized particles that are expected to have a widespread use, including cosmetics and medicines. METHODS: We investigated the association between intraperitoneal injection of pristine C60 fullerenes and vasomotor dysfunction in the aorta of 11-13 and 40-42 weeks old apolipoprotein E knockout mice (apoE-/-) with different degree of atherosclerosis. RESULTS: The aged apoE-/-mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aorta segments mounted in myographs and the phenylephrine-dependent vasoconstriction response was increased. One hour after an intraperitoneal injection of 0.05 or 0.5 mg/kg of C60 fullerenes, the young apoE-/- mice had slightly reduced maximal endothelium-dependent vasorelaxation. A similar tendency was observed in the old apoE-/- mice. Hampered endothelium-independent vasorelaxation was also observed as slightly increased EC50 of sodium nitroprusside-induced vasorelaxation response in young apoE-/- mice. CONCLUSION: Treatment with C60 fullerenes affected mainly the response to vasorelaxation in young apoE-/- mice, whereas the vasomotor dysfunction in old apoE-/- mice with more advanced atherosclerosis was less affected by acute C60 fullerene treatment. These findings represent an important step in the hazard characterization of C60 fullerenes by showing that intraperitoneal administration is associated with a moderate decrease in the vascular function of mice with atherosclerosis.",
author = "Vesterdal, {Lise K} and Folkmann, {Janne K} and Jacobsen, {Nicklas R} and Majid Sheykhzade and H{\aa}kan Wallin and Steffen Loft and Peter M{\o}ller",
year = "2009",
doi = "10.1186/1743-8977-6-5",
language = "English",
volume = "6",
pages = "5",
journal = "Particle and Fibre Toxicology",
issn = "1743-8977",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis

AU - Vesterdal, Lise K

AU - Folkmann, Janne K

AU - Jacobsen, Nicklas R

AU - Sheykhzade, Majid

AU - Wallin, Håkan

AU - Loft, Steffen

AU - Møller, Peter

PY - 2009

Y1 - 2009

N2 - ABSTRACT: BACKGROUND: Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C60 fullerenes are nano-sized particles that are expected to have a widespread use, including cosmetics and medicines. METHODS: We investigated the association between intraperitoneal injection of pristine C60 fullerenes and vasomotor dysfunction in the aorta of 11-13 and 40-42 weeks old apolipoprotein E knockout mice (apoE-/-) with different degree of atherosclerosis. RESULTS: The aged apoE-/-mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aorta segments mounted in myographs and the phenylephrine-dependent vasoconstriction response was increased. One hour after an intraperitoneal injection of 0.05 or 0.5 mg/kg of C60 fullerenes, the young apoE-/- mice had slightly reduced maximal endothelium-dependent vasorelaxation. A similar tendency was observed in the old apoE-/- mice. Hampered endothelium-independent vasorelaxation was also observed as slightly increased EC50 of sodium nitroprusside-induced vasorelaxation response in young apoE-/- mice. CONCLUSION: Treatment with C60 fullerenes affected mainly the response to vasorelaxation in young apoE-/- mice, whereas the vasomotor dysfunction in old apoE-/- mice with more advanced atherosclerosis was less affected by acute C60 fullerene treatment. These findings represent an important step in the hazard characterization of C60 fullerenes by showing that intraperitoneal administration is associated with a moderate decrease in the vascular function of mice with atherosclerosis.

AB - ABSTRACT: BACKGROUND: Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C60 fullerenes are nano-sized particles that are expected to have a widespread use, including cosmetics and medicines. METHODS: We investigated the association between intraperitoneal injection of pristine C60 fullerenes and vasomotor dysfunction in the aorta of 11-13 and 40-42 weeks old apolipoprotein E knockout mice (apoE-/-) with different degree of atherosclerosis. RESULTS: The aged apoE-/-mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aorta segments mounted in myographs and the phenylephrine-dependent vasoconstriction response was increased. One hour after an intraperitoneal injection of 0.05 or 0.5 mg/kg of C60 fullerenes, the young apoE-/- mice had slightly reduced maximal endothelium-dependent vasorelaxation. A similar tendency was observed in the old apoE-/- mice. Hampered endothelium-independent vasorelaxation was also observed as slightly increased EC50 of sodium nitroprusside-induced vasorelaxation response in young apoE-/- mice. CONCLUSION: Treatment with C60 fullerenes affected mainly the response to vasorelaxation in young apoE-/- mice, whereas the vasomotor dysfunction in old apoE-/- mice with more advanced atherosclerosis was less affected by acute C60 fullerene treatment. These findings represent an important step in the hazard characterization of C60 fullerenes by showing that intraperitoneal administration is associated with a moderate decrease in the vascular function of mice with atherosclerosis.

U2 - 10.1186/1743-8977-6-5

DO - 10.1186/1743-8977-6-5

M3 - Journal article

C2 - 19243580

VL - 6

SP - 5

JO - Particle and Fibre Toxicology

JF - Particle and Fibre Toxicology

SN - 1743-8977

ER -

ID: 12677548