Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system
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Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. / Mose, Tina; Kjaerstad, Mia Birkhoej; Mathiesen, Line; Nielsen, Jesper Bo; Edelfors, Sven; Knudsen, Lisbeth E.
In: Journal of Toxicology and Environmental Health. Part A: Current Issues, Vol. 71, No. 15, 2008, p. 984-91.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system
AU - Mose, Tina
AU - Kjaerstad, Mia Birkhoej
AU - Mathiesen, Line
AU - Nielsen, Jesper Bo
AU - Edelfors, Sven
AU - Knudsen, Lisbeth E.
PY - 2008
Y1 - 2008
N2 - Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15% glyphosate in maternal circulation crossed to the fetal circulation during the study period.
AB - Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15% glyphosate in maternal circulation crossed to the fetal circulation during the study period.
KW - Adult
KW - Benzoic Acid
KW - Caffeine
KW - Female
KW - Glycine
KW - Humans
KW - In Vitro Techniques
KW - Maternal-Fetal Exchange
KW - Models, Biological
KW - Perfusion
KW - Placenta
KW - Pregnancy
KW - Quantitative Structure-Activity Relationship
U2 - 10.1080/01932690801934513
DO - 10.1080/01932690801934513
M3 - Journal article
C2 - 18569607
VL - 71
SP - 984
EP - 991
JO - Journal of Toxicology and Environmental Health. Part A: Current Issues
JF - Journal of Toxicology and Environmental Health. Part A: Current Issues
SN - 1528-7394
IS - 15
ER -
ID: 137758144