Potassium bromate as positive assay control for the Fpg-modified comet assay

Research output: Contribution to journalJournal articlepeer-review

Standard

Potassium bromate as positive assay control for the Fpg-modified comet assay. / Møller, Peter; Muruzabal, Damian; Bakuradze, Tamara; Richling, Elke; Bankoglu, Ezgi Eyluel; Stopper, Helga; Langie, Sabine A.S.; Azqueta, Amaya; Jensen, Annie; Scavone, Francesca; Giovannelli, Lisa; Wojewódzka, Maria; Kruszewski, Marcin; Valdiglesias, Vanessa; Laffon, Blanca; Costa, Carla; Costa, Solange; Teixeira, João Paulo; Marino, Mirko; Del Bo', Cristian; Riso, Patrizia; Shaposhnikov, Sergey; Collins, Andrew.

In: Mutagenesis, Vol. 35, No. 4, 2020, p. 341-348.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Møller, P, Muruzabal, D, Bakuradze, T, Richling, E, Bankoglu, EE, Stopper, H, Langie, SAS, Azqueta, A, Jensen, A, Scavone, F, Giovannelli, L, Wojewódzka, M, Kruszewski, M, Valdiglesias, V, Laffon, B, Costa, C, Costa, S, Teixeira, JP, Marino, M, Del Bo', C, Riso, P, Shaposhnikov, S & Collins, A 2020, 'Potassium bromate as positive assay control for the Fpg-modified comet assay', Mutagenesis, vol. 35, no. 4, pp. 341-348. https://doi.org/10.1093/mutage/geaa011

APA

Møller, P., Muruzabal, D., Bakuradze, T., Richling, E., Bankoglu, E. E., Stopper, H., Langie, S. A. S., Azqueta, A., Jensen, A., Scavone, F., Giovannelli, L., Wojewódzka, M., Kruszewski, M., Valdiglesias, V., Laffon, B., Costa, C., Costa, S., Teixeira, J. P., Marino, M., ... Collins, A. (2020). Potassium bromate as positive assay control for the Fpg-modified comet assay. Mutagenesis, 35(4), 341-348. https://doi.org/10.1093/mutage/geaa011

Vancouver

Møller P, Muruzabal D, Bakuradze T, Richling E, Bankoglu EE, Stopper H et al. Potassium bromate as positive assay control for the Fpg-modified comet assay. Mutagenesis. 2020;35(4):341-348. https://doi.org/10.1093/mutage/geaa011

Author

Møller, Peter ; Muruzabal, Damian ; Bakuradze, Tamara ; Richling, Elke ; Bankoglu, Ezgi Eyluel ; Stopper, Helga ; Langie, Sabine A.S. ; Azqueta, Amaya ; Jensen, Annie ; Scavone, Francesca ; Giovannelli, Lisa ; Wojewódzka, Maria ; Kruszewski, Marcin ; Valdiglesias, Vanessa ; Laffon, Blanca ; Costa, Carla ; Costa, Solange ; Teixeira, João Paulo ; Marino, Mirko ; Del Bo', Cristian ; Riso, Patrizia ; Shaposhnikov, Sergey ; Collins, Andrew. / Potassium bromate as positive assay control for the Fpg-modified comet assay. In: Mutagenesis. 2020 ; Vol. 35, No. 4. pp. 341-348.

Bibtex

@article{d28810954c644603b87a64e465b42a1d,
title = "Potassium bromate as positive assay control for the Fpg-modified comet assay",
abstract = "The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We believe this might have been due to uncertainty as to what really constitutes a positive control. It should go without saying that a biomonitoring study cannot have a positive control group as it is unethical to expose healthy humans to DNA damaging (and thus potentially carcinogenic) agents. However, it is possible to include assay controls in the analysis (here meant as a cryopreserved sample of cells i.e. included in each experiment as a reference sample). In the present report we tested potassium bromate (KBrO3) as a positive comet assay control for the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay. Ten laboratories used the same procedure for treatment of monocytic THP-1 cells with KBrO3 (0.5, 1.5 and 4.5 mM for 1 h at 37°C) and subsequent cryopreservation. Results from one laboratory were excluded in the statistical analysis because of technical issues in the Fpg-modified comet assay. All other laboratories found a concentration-response relationship in cryopreserved samples (regression coefficients from 0.80 to 0.98), although with different slopes ranging from 1.25 to 11.9 Fpg-sensitive sites (%DNA in tail) per 1 mM KBrO3. Our results demonstrate that KBrO3 is a suitable positive comet assay control.",
author = "Peter M{\o}ller and Damian Muruzabal and Tamara Bakuradze and Elke Richling and Bankoglu, {Ezgi Eyluel} and Helga Stopper and Langie, {Sabine A.S.} and Amaya Azqueta and Annie Jensen and Francesca Scavone and Lisa Giovannelli and Maria Wojew{\'o}dzka and Marcin Kruszewski and Vanessa Valdiglesias and Blanca Laffon and Carla Costa and Solange Costa and Teixeira, {Jo{\~a}o Paulo} and Mirko Marino and {Del Bo'}, Cristian and Patrizia Riso and Sergey Shaposhnikov and Andrew Collins",
year = "2020",
doi = "10.1093/mutage/geaa011",
language = "English",
volume = "35",
pages = "341--348",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - Potassium bromate as positive assay control for the Fpg-modified comet assay

AU - Møller, Peter

AU - Muruzabal, Damian

AU - Bakuradze, Tamara

AU - Richling, Elke

AU - Bankoglu, Ezgi Eyluel

AU - Stopper, Helga

AU - Langie, Sabine A.S.

AU - Azqueta, Amaya

AU - Jensen, Annie

AU - Scavone, Francesca

AU - Giovannelli, Lisa

AU - Wojewódzka, Maria

AU - Kruszewski, Marcin

AU - Valdiglesias, Vanessa

AU - Laffon, Blanca

AU - Costa, Carla

AU - Costa, Solange

AU - Teixeira, João Paulo

AU - Marino, Mirko

AU - Del Bo', Cristian

AU - Riso, Patrizia

AU - Shaposhnikov, Sergey

AU - Collins, Andrew

PY - 2020

Y1 - 2020

N2 - The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We believe this might have been due to uncertainty as to what really constitutes a positive control. It should go without saying that a biomonitoring study cannot have a positive control group as it is unethical to expose healthy humans to DNA damaging (and thus potentially carcinogenic) agents. However, it is possible to include assay controls in the analysis (here meant as a cryopreserved sample of cells i.e. included in each experiment as a reference sample). In the present report we tested potassium bromate (KBrO3) as a positive comet assay control for the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay. Ten laboratories used the same procedure for treatment of monocytic THP-1 cells with KBrO3 (0.5, 1.5 and 4.5 mM for 1 h at 37°C) and subsequent cryopreservation. Results from one laboratory were excluded in the statistical analysis because of technical issues in the Fpg-modified comet assay. All other laboratories found a concentration-response relationship in cryopreserved samples (regression coefficients from 0.80 to 0.98), although with different slopes ranging from 1.25 to 11.9 Fpg-sensitive sites (%DNA in tail) per 1 mM KBrO3. Our results demonstrate that KBrO3 is a suitable positive comet assay control.

AB - The comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We believe this might have been due to uncertainty as to what really constitutes a positive control. It should go without saying that a biomonitoring study cannot have a positive control group as it is unethical to expose healthy humans to DNA damaging (and thus potentially carcinogenic) agents. However, it is possible to include assay controls in the analysis (here meant as a cryopreserved sample of cells i.e. included in each experiment as a reference sample). In the present report we tested potassium bromate (KBrO3) as a positive comet assay control for the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay. Ten laboratories used the same procedure for treatment of monocytic THP-1 cells with KBrO3 (0.5, 1.5 and 4.5 mM for 1 h at 37°C) and subsequent cryopreservation. Results from one laboratory were excluded in the statistical analysis because of technical issues in the Fpg-modified comet assay. All other laboratories found a concentration-response relationship in cryopreserved samples (regression coefficients from 0.80 to 0.98), although with different slopes ranging from 1.25 to 11.9 Fpg-sensitive sites (%DNA in tail) per 1 mM KBrO3. Our results demonstrate that KBrO3 is a suitable positive comet assay control.

U2 - 10.1093/mutage/geaa011

DO - 10.1093/mutage/geaa011

M3 - Journal article

C2 - 32319518

AN - SCOPUS:85086330319

VL - 35

SP - 341

EP - 348

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 4

ER -

ID: 255882268