Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity

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Standard

Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity. / Lykkesfeldt, Jens; Loft, Steffen; Poulsen, Henrik E.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 660, No. 1, 03.10.1994, p. 23-29.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Lykkesfeldt, J, Loft, S & Poulsen, HE 1994, 'Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 660, no. 1, pp. 23-29. https://doi.org/10.1016/0378-4347(94)00265-7

APA

Lykkesfeldt, J., Loft, S., & Poulsen, H. E. (1994). Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity. Journal of Chromatography B: Biomedical Sciences and Applications, 660(1), 23-29. https://doi.org/10.1016/0378-4347(94)00265-7

Vancouver

Lykkesfeldt J, Loft S, Poulsen HE. Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity. Journal of Chromatography B: Biomedical Sciences and Applications. 1994 Oct 3;660(1):23-29. https://doi.org/10.1016/0378-4347(94)00265-7

Author

Lykkesfeldt, Jens ; Loft, Steffen ; Poulsen, Henrik E. / Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1994 ; Vol. 660, No. 1. pp. 23-29.

Bibtex

@article{5271ced0803f43a0bbf3d8308626a782,
title = "Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity",
abstract = "The ratio of the hydrophilic metabolite 6β-hydroxycortisol to its parent compound cortisol has recently been demonstrated to be a specific marker for human CYP3A oxygenase activity. We have developed a sensitive and simple single-run high-performance liquid chromatographic method for the quantification of urinary free cortisol and 6β-hydroxycortisol using dexamethasone as internal standard. The urine samples (1 ml) are applied to Sep-Pak cartridges, which are washed with water and eluted with ethyl acetate-diethyl ether (4:1, v/v). The organic extracts are washed sequentially with alkaline and acidic solutions saturated with sodium sulfate and subsequently concentrated to dryness. After reconstitution in ethanolic water, the samples are analyzed on a reversed-phase gradient system using ultraviolet absorbance detection at 254 nm. The within- and between-day coefficients of variation (C.V.) for the assay where both in the range of 5-10{\%}. The reference interval for the 6β-hydroxycortisol/cortisol ratio of eleven healthy non-smoking subjects was 2.77-26.88 with an average of 10.09 ± 6.89 (S.D.). The method constitutes an improvement over previous methods and is suitable for routine assessment of the 6β-hydroxycortisol/cortisol ratio requiring only 1 ml of urine or less.",
author = "Jens Lykkesfeldt and Steffen Loft and Poulsen, {Henrik E.}",
year = "1994",
month = "10",
day = "3",
doi = "10.1016/0378-4347(94)00265-7",
language = "English",
volume = "660",
pages = "23--29",
journal = "Journal of Chromatography - Biomedical Applications",
issn = "0378-4347",
publisher = "Elsevier",
number = "1",

}

RIS

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T1 - Simultaneous determination of urinary free cortisol and 6β-hydroxycortisol by high-performance liquid chromatography to measure human CYP3A activity

AU - Lykkesfeldt, Jens

AU - Loft, Steffen

AU - Poulsen, Henrik E.

PY - 1994/10/3

Y1 - 1994/10/3

N2 - The ratio of the hydrophilic metabolite 6β-hydroxycortisol to its parent compound cortisol has recently been demonstrated to be a specific marker for human CYP3A oxygenase activity. We have developed a sensitive and simple single-run high-performance liquid chromatographic method for the quantification of urinary free cortisol and 6β-hydroxycortisol using dexamethasone as internal standard. The urine samples (1 ml) are applied to Sep-Pak cartridges, which are washed with water and eluted with ethyl acetate-diethyl ether (4:1, v/v). The organic extracts are washed sequentially with alkaline and acidic solutions saturated with sodium sulfate and subsequently concentrated to dryness. After reconstitution in ethanolic water, the samples are analyzed on a reversed-phase gradient system using ultraviolet absorbance detection at 254 nm. The within- and between-day coefficients of variation (C.V.) for the assay where both in the range of 5-10%. The reference interval for the 6β-hydroxycortisol/cortisol ratio of eleven healthy non-smoking subjects was 2.77-26.88 with an average of 10.09 ± 6.89 (S.D.). The method constitutes an improvement over previous methods and is suitable for routine assessment of the 6β-hydroxycortisol/cortisol ratio requiring only 1 ml of urine or less.

AB - The ratio of the hydrophilic metabolite 6β-hydroxycortisol to its parent compound cortisol has recently been demonstrated to be a specific marker for human CYP3A oxygenase activity. We have developed a sensitive and simple single-run high-performance liquid chromatographic method for the quantification of urinary free cortisol and 6β-hydroxycortisol using dexamethasone as internal standard. The urine samples (1 ml) are applied to Sep-Pak cartridges, which are washed with water and eluted with ethyl acetate-diethyl ether (4:1, v/v). The organic extracts are washed sequentially with alkaline and acidic solutions saturated with sodium sulfate and subsequently concentrated to dryness. After reconstitution in ethanolic water, the samples are analyzed on a reversed-phase gradient system using ultraviolet absorbance detection at 254 nm. The within- and between-day coefficients of variation (C.V.) for the assay where both in the range of 5-10%. The reference interval for the 6β-hydroxycortisol/cortisol ratio of eleven healthy non-smoking subjects was 2.77-26.88 with an average of 10.09 ± 6.89 (S.D.). The method constitutes an improvement over previous methods and is suitable for routine assessment of the 6β-hydroxycortisol/cortisol ratio requiring only 1 ml of urine or less.

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U2 - 10.1016/0378-4347(94)00265-7

DO - 10.1016/0378-4347(94)00265-7

M3 - Journal article

C2 - 7858720

AN - SCOPUS:0028131815

VL - 660

SP - 23

EP - 29

JO - Journal of Chromatography - Biomedical Applications

JF - Journal of Chromatography - Biomedical Applications

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