The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum: hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum : hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency. / Juul, A; Møller, S; Mosfeldt-Laursen, E; Rasmussen, M H; Scheike, Thomas Harder; Pedersen, S A; Kastrup, K W; Yu, Hao; Mistry, J; Rasmussen, S; Müller, J; Henriksen, J; Skakkebaek, N E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 83, No. 12, 1998, p. 4408-15.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Juul, A, Møller, S, Mosfeldt-Laursen, E, Rasmussen, MH, Scheike, TH, Pedersen, SA, Kastrup, KW, Yu, H, Mistry, J, Rasmussen, S, Müller, J, Henriksen, J & Skakkebaek, NE 1998, 'The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum: hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency', Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 12, pp. 4408-15.

APA

Juul, A., Møller, S., Mosfeldt-Laursen, E., Rasmussen, M. H., Scheike, T. H., Pedersen, S. A., ... Skakkebaek, N. E. (1998). The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum: hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency. Journal of Clinical Endocrinology and Metabolism, 83(12), 4408-15.

Vancouver

Juul A, Møller S, Mosfeldt-Laursen E, Rasmussen MH, Scheike TH, Pedersen SA et al. The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum: hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency. Journal of Clinical Endocrinology and Metabolism. 1998;83(12):4408-15.

Author

Juul, A ; Møller, S ; Mosfeldt-Laursen, E ; Rasmussen, M H ; Scheike, Thomas Harder ; Pedersen, S A ; Kastrup, K W ; Yu, Hao ; Mistry, J ; Rasmussen, S ; Müller, J ; Henriksen, J ; Skakkebaek, N E. / The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum : hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency. In: Journal of Clinical Endocrinology and Metabolism. 1998 ; Vol. 83, No. 12. pp. 4408-15.

Bibtex

@article{2c24cb95458d4d21afedf9f7d673afac,
title = "The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum: hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency",
abstract = "Circulating insulin-like growth factor-I (IGF-I) is predominantly bound in the trimeric complex comprised of IGF binding protein-3 (IGFBP-3) and acid-labile subunit (ALS). Circulating concentrations of IGF-I, IGFBP-3 and ALS are believed to reflect the GH secretory status, but the clinical use of ALS determination is not known. We therefore, determined the: 1) hepatosplanchnic release of ALS by liver vein catheterization (n=30); 2) 24-h diurnal variation of ALS (n=8); 3) normal age-related ranges of circulating ALS (n=1158); 4) diagnostic value of ALS in 108 patients with childhood-onset GH deficiency (GHD). We found: 1) no significant arteriovenous gradient over the liver ofALS, IGF-I, and IGFBP-3; 2) the diurnal variation of ALS was 12{\%} (mean coefficient of variation percent); 3) ALS levels increased throughout childhood with maximal levels in puberty, with a subsequent decrease with age in adults; and 4) ALS levels were below -2 SD in 57 of 79 GHD patients (sensitivity 72{\%}) and above 2 SD in 22 of 29 patients with normal GH response (specificity 76{\%}), which was similar, compared with the diagnostic utility of IGF-I and IGFBP-3. Finally, our findings indicate that hepatic ALS production is not measurable by this approach or, alternatively, that the liver is not the primary source of circulating ALS, IGF-I, or IGFBP-3 in humans. In conclusion, we have provided extensive normal data for a novel ALS assay and found that circulating ALS levels exhibit minor diurnal variation. We suggest that ALS determination may be used in future classification of adults suspected of GHD.",
author = "A Juul and S M{\o}ller and E Mosfeldt-Laursen and Rasmussen, {M H} and Scheike, {Thomas Harder} and Pedersen, {S A} and Kastrup, {K W} and Hao Yu and J Mistry and S Rasmussen and J M{\"u}ller and J Henriksen and Skakkebaek, {N E}",
year = "1998",
language = "English",
volume = "83",
pages = "4408--15",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",
number = "12",

}

RIS

TY - JOUR

T1 - The acid-labile subunit of human ternary insulin-like growth factor binding protein complex in serum

T2 - hepatosplanchnic release, diurnal variation, circulating concentrations in healthy subjects, and diagnostic use in patients with growth hormone deficiency

AU - Juul, A

AU - Møller, S

AU - Mosfeldt-Laursen, E

AU - Rasmussen, M H

AU - Scheike, Thomas Harder

AU - Pedersen, S A

AU - Kastrup, K W

AU - Yu, Hao

AU - Mistry, J

AU - Rasmussen, S

AU - Müller, J

AU - Henriksen, J

AU - Skakkebaek, N E

PY - 1998

Y1 - 1998

N2 - Circulating insulin-like growth factor-I (IGF-I) is predominantly bound in the trimeric complex comprised of IGF binding protein-3 (IGFBP-3) and acid-labile subunit (ALS). Circulating concentrations of IGF-I, IGFBP-3 and ALS are believed to reflect the GH secretory status, but the clinical use of ALS determination is not known. We therefore, determined the: 1) hepatosplanchnic release of ALS by liver vein catheterization (n=30); 2) 24-h diurnal variation of ALS (n=8); 3) normal age-related ranges of circulating ALS (n=1158); 4) diagnostic value of ALS in 108 patients with childhood-onset GH deficiency (GHD). We found: 1) no significant arteriovenous gradient over the liver ofALS, IGF-I, and IGFBP-3; 2) the diurnal variation of ALS was 12% (mean coefficient of variation percent); 3) ALS levels increased throughout childhood with maximal levels in puberty, with a subsequent decrease with age in adults; and 4) ALS levels were below -2 SD in 57 of 79 GHD patients (sensitivity 72%) and above 2 SD in 22 of 29 patients with normal GH response (specificity 76%), which was similar, compared with the diagnostic utility of IGF-I and IGFBP-3. Finally, our findings indicate that hepatic ALS production is not measurable by this approach or, alternatively, that the liver is not the primary source of circulating ALS, IGF-I, or IGFBP-3 in humans. In conclusion, we have provided extensive normal data for a novel ALS assay and found that circulating ALS levels exhibit minor diurnal variation. We suggest that ALS determination may be used in future classification of adults suspected of GHD.

AB - Circulating insulin-like growth factor-I (IGF-I) is predominantly bound in the trimeric complex comprised of IGF binding protein-3 (IGFBP-3) and acid-labile subunit (ALS). Circulating concentrations of IGF-I, IGFBP-3 and ALS are believed to reflect the GH secretory status, but the clinical use of ALS determination is not known. We therefore, determined the: 1) hepatosplanchnic release of ALS by liver vein catheterization (n=30); 2) 24-h diurnal variation of ALS (n=8); 3) normal age-related ranges of circulating ALS (n=1158); 4) diagnostic value of ALS in 108 patients with childhood-onset GH deficiency (GHD). We found: 1) no significant arteriovenous gradient over the liver ofALS, IGF-I, and IGFBP-3; 2) the diurnal variation of ALS was 12% (mean coefficient of variation percent); 3) ALS levels increased throughout childhood with maximal levels in puberty, with a subsequent decrease with age in adults; and 4) ALS levels were below -2 SD in 57 of 79 GHD patients (sensitivity 72%) and above 2 SD in 22 of 29 patients with normal GH response (specificity 76%), which was similar, compared with the diagnostic utility of IGF-I and IGFBP-3. Finally, our findings indicate that hepatic ALS production is not measurable by this approach or, alternatively, that the liver is not the primary source of circulating ALS, IGF-I, or IGFBP-3 in humans. In conclusion, we have provided extensive normal data for a novel ALS assay and found that circulating ALS levels exhibit minor diurnal variation. We suggest that ALS determination may be used in future classification of adults suspected of GHD.

M3 - Journal article

VL - 83

SP - 4408

EP - 4415

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 12

ER -

ID: 48486254