Validation of biomarkers for the study of environmental carcinogens: a review
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Validation of biomarkers for the study of environmental carcinogens: a review. / Gallo, Valentina; Khan, Aneire; Gonzales, Carlos; Phillips, David H; Schoket, Bernadette; Györffy, Erika; Anna, Livia; Kovács, Katalin; Møller, Peter; Loft, Steffen; Kyrtopoulos, Soterios; Matullo, Giuseppe; Vineis, Paolo.
In: Biomarkers, Vol. 13, No. 5, 2008, p. 505-34.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Validation of biomarkers for the study of environmental carcinogens: a review
AU - Gallo, Valentina
AU - Khan, Aneire
AU - Gonzales, Carlos
AU - Phillips, David H
AU - Schoket, Bernadette
AU - Györffy, Erika
AU - Anna, Livia
AU - Kovács, Katalin
AU - Møller, Peter
AU - Loft, Steffen
AU - Kyrtopoulos, Soterios
AU - Matullo, Giuseppe
AU - Vineis, Paolo
N1 - Keywords: Biological Markers; Carcinogens, Environmental; DNA Adducts; DNA Damage; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Humans; Oxidative Stress
PY - 2008
Y1 - 2008
N2 - There is a need for validation of biomarkers. Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA. A systematic literature search in PubMed was performed. Information on the variability and reliability of the laboratory tests used for biomarkers measurements was collected. For the evaluation of the evidence on validation we referred to the ACCE criteria. Little is known about intraindividual variation of DNA adduct measurements, but measurements have a good repeatability irrespective of the technique used for their identification; reproducibility improved after the correction for a laboratory factor. A high-sensitivity method is available for the measurement of 1-hydroxypyrene in urine. There is consensus on validation of biomarkers of oxidative damage DNA based on the comet assay and chromatographic measurement in blood while urinary measurements by chromatographic assays are well validated, and ELISA-based assays appear to lack specificity. Immunoassays for the quantification of adducts of N-nitroso compounds are useful for large epidemiological studies, given their sensitivity, the small amount of DNA required and their potential for rapid and high-throughput analysis.
AB - There is a need for validation of biomarkers. Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA. A systematic literature search in PubMed was performed. Information on the variability and reliability of the laboratory tests used for biomarkers measurements was collected. For the evaluation of the evidence on validation we referred to the ACCE criteria. Little is known about intraindividual variation of DNA adduct measurements, but measurements have a good repeatability irrespective of the technique used for their identification; reproducibility improved after the correction for a laboratory factor. A high-sensitivity method is available for the measurement of 1-hydroxypyrene in urine. There is consensus on validation of biomarkers of oxidative damage DNA based on the comet assay and chromatographic measurement in blood while urinary measurements by chromatographic assays are well validated, and ELISA-based assays appear to lack specificity. Immunoassays for the quantification of adducts of N-nitroso compounds are useful for large epidemiological studies, given their sensitivity, the small amount of DNA required and their potential for rapid and high-throughput analysis.
M3 - Journal article
C2 - 18979642
VL - 13
SP - 505
EP - 534
JO - Biomarkers
JF - Biomarkers
SN - 1354-750X
IS - 5
ER -
ID: 9910593