Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone

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Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone. / Jensen, Marie Aarrebo; Hansen, Åse Marie; Abrahamsson, Peter; Nørgaard, Asger W.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 879, No. 25, 2011, p. 2527-32.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jensen, MA, Hansen, ÅM, Abrahamsson, P & Nørgaard, AW 2011, 'Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 879, no. 25, pp. 2527-32. https://doi.org/10.1016/j.jchromb.2011.07.005

APA

Jensen, M. A., Hansen, Å. M., Abrahamsson, P., & Nørgaard, A. W. (2011). Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 879(25), 2527-32. https://doi.org/10.1016/j.jchromb.2011.07.005

Vancouver

Jensen MA, Hansen ÅM, Abrahamsson P, Nørgaard AW. Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2011;879(25):2527-32. https://doi.org/10.1016/j.jchromb.2011.07.005

Author

Jensen, Marie Aarrebo ; Hansen, Åse Marie ; Abrahamsson, Peter ; Nørgaard, Asger W. / Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2011 ; Vol. 879, No. 25. pp. 2527-32.

Bibtex

@article{000f77f7e06b454a9fce1f1ff5a06d80,
title = "Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone",
abstract = "Circadian disruption can have several possible health consequences, but is not well studied. In order to measure circadian disruption, in relation to shift or night work, we developed a simple and sensitive method for the simultaneous determination of melatonin, cortisol and testosterone in human saliva. We used liquid-liquid extraction (LLE) followed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS) recorded in positive ion mode. Saliva samples were collected by spitting directly into tubes and 250 µL were used for analysis. The limits of detection were 4.1 pmol/L, 0.27 nmol/L and 10.8 pmol/L for melatonin, cortisol, and testosterone, respectively. The developed method was sensitive enough to measure circadian rhythms of all 3 hormones in a pilot study among four healthy volunteers. It can therefor be used to study the impact of night work and working in artificial light on the workers circadian rhythms. To our knowledge this is the first LC-ESI-MS/MS method for simultaneous determination of salivary melatonin, cortisol and testosterone.",
keywords = "Adult, Chromatography, Liquid, Circadian Rhythm, Drug Stability, Female, Humans, Hydrocortisone, Male, Melatonin, Middle Aged, Reproducibility of Results, Saliva, Tandem Mass Spectrometry, Testosterone",
author = "Jensen, {Marie Aarrebo} and Hansen, {{\AA}se Marie} and Peter Abrahamsson and N{\o}rgaard, {Asger W}",
note = "Copyright {\circledC} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
doi = "10.1016/j.jchromb.2011.07.005",
language = "English",
volume = "879",
pages = "2527--32",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "25",

}

RIS

TY - JOUR

T1 - Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone

AU - Jensen, Marie Aarrebo

AU - Hansen, Åse Marie

AU - Abrahamsson, Peter

AU - Nørgaard, Asger W

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011

Y1 - 2011

N2 - Circadian disruption can have several possible health consequences, but is not well studied. In order to measure circadian disruption, in relation to shift or night work, we developed a simple and sensitive method for the simultaneous determination of melatonin, cortisol and testosterone in human saliva. We used liquid-liquid extraction (LLE) followed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS) recorded in positive ion mode. Saliva samples were collected by spitting directly into tubes and 250 µL were used for analysis. The limits of detection were 4.1 pmol/L, 0.27 nmol/L and 10.8 pmol/L for melatonin, cortisol, and testosterone, respectively. The developed method was sensitive enough to measure circadian rhythms of all 3 hormones in a pilot study among four healthy volunteers. It can therefor be used to study the impact of night work and working in artificial light on the workers circadian rhythms. To our knowledge this is the first LC-ESI-MS/MS method for simultaneous determination of salivary melatonin, cortisol and testosterone.

AB - Circadian disruption can have several possible health consequences, but is not well studied. In order to measure circadian disruption, in relation to shift or night work, we developed a simple and sensitive method for the simultaneous determination of melatonin, cortisol and testosterone in human saliva. We used liquid-liquid extraction (LLE) followed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS) recorded in positive ion mode. Saliva samples were collected by spitting directly into tubes and 250 µL were used for analysis. The limits of detection were 4.1 pmol/L, 0.27 nmol/L and 10.8 pmol/L for melatonin, cortisol, and testosterone, respectively. The developed method was sensitive enough to measure circadian rhythms of all 3 hormones in a pilot study among four healthy volunteers. It can therefor be used to study the impact of night work and working in artificial light on the workers circadian rhythms. To our knowledge this is the first LC-ESI-MS/MS method for simultaneous determination of salivary melatonin, cortisol and testosterone.

KW - Adult

KW - Chromatography, Liquid

KW - Circadian Rhythm

KW - Drug Stability

KW - Female

KW - Humans

KW - Hydrocortisone

KW - Male

KW - Melatonin

KW - Middle Aged

KW - Reproducibility of Results

KW - Saliva

KW - Tandem Mass Spectrometry

KW - Testosterone

U2 - 10.1016/j.jchromb.2011.07.005

DO - 10.1016/j.jchromb.2011.07.005

M3 - Journal article

VL - 879

SP - 2527

EP - 2532

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 25

ER -

ID: 37472858