Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. / Crameri, Regina M; Langberg, Henning; Magnusson, Peter; Jensen, Charlotte H; Schrøder, Henrik Daa; Olesen, Jens L; Suetta, Charlotte; Teisner, Børge; Kjaer, Michael.

In: Journal of Physiology, Vol. 558, No. Pt 1, 2004, p. 333-40.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Crameri, RM, Langberg, H, Magnusson, P, Jensen, CH, Schrøder, HD, Olesen, JL, Suetta, C, Teisner, B & Kjaer, M 2004, 'Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise', Journal of Physiology, vol. 558, no. Pt 1, pp. 333-40. https://doi.org/10.1113/jphysiol.2004.061846

APA

Crameri, R. M., Langberg, H., Magnusson, P., Jensen, C. H., Schrøder, H. D., Olesen, J. L., ... Kjaer, M. (2004). Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. Journal of Physiology, 558(Pt 1), 333-40. https://doi.org/10.1113/jphysiol.2004.061846

Vancouver

Crameri RM, Langberg H, Magnusson P, Jensen CH, Schrøder HD, Olesen JL et al. Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. Journal of Physiology. 2004;558(Pt 1):333-40. https://doi.org/10.1113/jphysiol.2004.061846

Author

Crameri, Regina M ; Langberg, Henning ; Magnusson, Peter ; Jensen, Charlotte H ; Schrøder, Henrik Daa ; Olesen, Jens L ; Suetta, Charlotte ; Teisner, Børge ; Kjaer, Michael. / Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. In: Journal of Physiology. 2004 ; Vol. 558, No. Pt 1. pp. 333-40.

Bibtex

@article{75ee301958694d05a3934fd52febc2e8,
title = "Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise",
abstract = "No studies to date have reported activation of satellite cells in vivo in human muscle after a single bout of high intensity exercise. In this investigation, eight individuals performed a single bout of high intensity exercise with one leg, the contralateral leg being the control. A significant increase in mononuclear cells staining for the neural cell adhesion molecule (N-CAM) and fetal antigen 1 (FA1) were observed within the exercised human vastus lateralis muscle on days 4 and 8 post exercise. In addition, a significant increase in the concentration of the FA1 protein was determined in intramuscular dialysate samples taken from the vastus lateralis muscle of the exercising leg (day 0: 1.89 +/- 0.82 ng ml(-1); day 2: 1.68 +/- 0.37 ng ml(-1); day 4: 3.26 +/- 1.29 ng ml(-1), P <0.05 versus basal; day 8: 4.68 +/- 2.06 ng ml(-1), P <0.05 versus basal and control). No change was noted in the control leg. Despite this increase in N-CAM- and FA1-positive mononuclear cells, an increased expression of myogenin and the neonatal isoform of the myosin heavy chain (MHCn) was not observed. Interestingly, myofibre lesions resulting from extensive damage to the proteins within the myofibre, particularly desmin or dystrophin, were not observed, and hence did not appear to induce the expression of either N-CAM or FA1. We therefore propose that satellite cells can be induced to re-enter the cell growth cycle after a single bout of unaccustomed high intensity exercise. However, a single bout of exercise is not sufficient for the satellite cell to undergo terminal differentiation.",
keywords = "Adult, Cell Count, Cell Differentiation, Exercise, Glycoproteins, Humans, Male, Muscle Contraction, Muscle Fibers, Skeletal, Muscle, Skeletal, Neural Cell Adhesion Molecules, Satellite Cells, Skeletal Muscle",
author = "Crameri, {Regina M} and Henning Langberg and Peter Magnusson and Jensen, {Charlotte H} and Schr{\o}der, {Henrik Daa} and Olesen, {Jens L} and Charlotte Suetta and B{\o}rge Teisner and Michael Kjaer",
year = "2004",
doi = "10.1113/jphysiol.2004.061846",
language = "English",
volume = "558",
pages = "333--40",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "Pt 1",

}

RIS

TY - JOUR

T1 - Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise

AU - Crameri, Regina M

AU - Langberg, Henning

AU - Magnusson, Peter

AU - Jensen, Charlotte H

AU - Schrøder, Henrik Daa

AU - Olesen, Jens L

AU - Suetta, Charlotte

AU - Teisner, Børge

AU - Kjaer, Michael

PY - 2004

Y1 - 2004

N2 - No studies to date have reported activation of satellite cells in vivo in human muscle after a single bout of high intensity exercise. In this investigation, eight individuals performed a single bout of high intensity exercise with one leg, the contralateral leg being the control. A significant increase in mononuclear cells staining for the neural cell adhesion molecule (N-CAM) and fetal antigen 1 (FA1) were observed within the exercised human vastus lateralis muscle on days 4 and 8 post exercise. In addition, a significant increase in the concentration of the FA1 protein was determined in intramuscular dialysate samples taken from the vastus lateralis muscle of the exercising leg (day 0: 1.89 +/- 0.82 ng ml(-1); day 2: 1.68 +/- 0.37 ng ml(-1); day 4: 3.26 +/- 1.29 ng ml(-1), P <0.05 versus basal; day 8: 4.68 +/- 2.06 ng ml(-1), P <0.05 versus basal and control). No change was noted in the control leg. Despite this increase in N-CAM- and FA1-positive mononuclear cells, an increased expression of myogenin and the neonatal isoform of the myosin heavy chain (MHCn) was not observed. Interestingly, myofibre lesions resulting from extensive damage to the proteins within the myofibre, particularly desmin or dystrophin, were not observed, and hence did not appear to induce the expression of either N-CAM or FA1. We therefore propose that satellite cells can be induced to re-enter the cell growth cycle after a single bout of unaccustomed high intensity exercise. However, a single bout of exercise is not sufficient for the satellite cell to undergo terminal differentiation.

AB - No studies to date have reported activation of satellite cells in vivo in human muscle after a single bout of high intensity exercise. In this investigation, eight individuals performed a single bout of high intensity exercise with one leg, the contralateral leg being the control. A significant increase in mononuclear cells staining for the neural cell adhesion molecule (N-CAM) and fetal antigen 1 (FA1) were observed within the exercised human vastus lateralis muscle on days 4 and 8 post exercise. In addition, a significant increase in the concentration of the FA1 protein was determined in intramuscular dialysate samples taken from the vastus lateralis muscle of the exercising leg (day 0: 1.89 +/- 0.82 ng ml(-1); day 2: 1.68 +/- 0.37 ng ml(-1); day 4: 3.26 +/- 1.29 ng ml(-1), P <0.05 versus basal; day 8: 4.68 +/- 2.06 ng ml(-1), P <0.05 versus basal and control). No change was noted in the control leg. Despite this increase in N-CAM- and FA1-positive mononuclear cells, an increased expression of myogenin and the neonatal isoform of the myosin heavy chain (MHCn) was not observed. Interestingly, myofibre lesions resulting from extensive damage to the proteins within the myofibre, particularly desmin or dystrophin, were not observed, and hence did not appear to induce the expression of either N-CAM or FA1. We therefore propose that satellite cells can be induced to re-enter the cell growth cycle after a single bout of unaccustomed high intensity exercise. However, a single bout of exercise is not sufficient for the satellite cell to undergo terminal differentiation.

KW - Adult

KW - Cell Count

KW - Cell Differentiation

KW - Exercise

KW - Glycoproteins

KW - Humans

KW - Male

KW - Muscle Contraction

KW - Muscle Fibers, Skeletal

KW - Muscle, Skeletal

KW - Neural Cell Adhesion Molecules

KW - Satellite Cells, Skeletal Muscle

U2 - 10.1113/jphysiol.2004.061846

DO - 10.1113/jphysiol.2004.061846

M3 - Journal article

VL - 558

SP - 333

EP - 340

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - Pt 1

ER -

ID: 38367232