Early osteoarthritis and microdialysis: a novel in vivo approach for measurements of biochemical markers in the perisynovium and intraarticularly

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Ida Carøe Helmark, U R Mikkelsen, M R Krogsgaard, B Belhage, M C H Petersen, Henning Langberg, M Kjaer

The microdialysis technique was evaluated as a possible method to obtain local measurements of biochemical markers from knee joints with degenerative changes. Seven patients scheduled for arthroscopy of the knee due to minor to moderate degenerative changes had microdialysis catheters inserted under ultrasonographic guidance, intraarticularly and in the synovium-close tissue. Catheters were perfused at a rate of 2 µl/min for approximately 100 min with a Ringer solution containing radioactively labeled glucose, and the positions of the catheters were later visualized during arthroscopy. All intraarticular catheters and 6/7 subsynovial catheters were positioned correctly. Relative recovery (RR) was intraarticularly 0.64 ± 0.02 (mean ± SEM) and synovium-close 0.54 ± 0.06. Mean values of cartilage oligomeric matrix protein (COMP), aggrecan and glucosyl-galactosyl-pyridinoline in the intraarticular dialysates were 18.1 ± 7.0 U/l, 243.6 ± 108.6 ng/ml and 108.0 ± 29.0 pmol/ml, respectively. COMP and glucosyl-galactosyl-pyridinoline concentrations were significantly higher intraarticularly compared to perisynovial tissue (P <0.05), whereas for aggrecan, no significant difference was found (P = 0.06). The microdialysis method can be used for intraarticular and subsynovial determination of metabolites in human knees at these sites. The present methodology displays a potential for future studies of simultaneous biochemical changes within and around joints.
Original languageEnglish
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Volume18
Issue number11
Pages (from-to)1617-23
Number of pages7
ISSN0942-2056
DOIs
Publication statusPublished - 2010

    Research areas

  • Adult, Aged, Aggrecans, Amino Acids, Arthroscopy, Biological Markers, Catheters, Early Diagnosis, Enzyme-Linked Immunosorbent Assay, Extracellular Matrix Proteins, Female, Glycoproteins, Humans, Knee Joint, Male, Microdialysis, Middle Aged, Osteoarthritis, Knee, Sampling Studies, Sensitivity and Specificity, Severity of Illness Index, Synovial Membrane

ID: 38364546