Effect of acute exercise on patella tendon protein synthesis and gene expression

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Effect of acute exercise on patella tendon protein synthesis and gene expression. / Dideriksen, Kasper; Sindby, Ann Kathrine Ryberg; Krogsgaard, Michael; Schjerling, Peter; Holm, Lars; Langberg, Henning.

In: SpringerPlus, Vol. 2, 12.2013, p. 1-108.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Dideriksen, K, Sindby, AKR, Krogsgaard, M, Schjerling, P, Holm, L & Langberg, H 2013, 'Effect of acute exercise on patella tendon protein synthesis and gene expression', SpringerPlus, vol. 2, pp. 1-108. https://doi.org/10.1186/2193-1801-2-109

APA

Dideriksen, K., Sindby, A. K. R., Krogsgaard, M., Schjerling, P., Holm, L., & Langberg, H. (2013). Effect of acute exercise on patella tendon protein synthesis and gene expression. SpringerPlus, 2, 1-108. https://doi.org/10.1186/2193-1801-2-109

Vancouver

Dideriksen K, Sindby AKR, Krogsgaard M, Schjerling P, Holm L, Langberg H. Effect of acute exercise on patella tendon protein synthesis and gene expression. SpringerPlus. 2013 Dec;2:1-108. https://doi.org/10.1186/2193-1801-2-109

Author

Dideriksen, Kasper ; Sindby, Ann Kathrine Ryberg ; Krogsgaard, Michael ; Schjerling, Peter ; Holm, Lars ; Langberg, Henning. / Effect of acute exercise on patella tendon protein synthesis and gene expression. In: SpringerPlus. 2013 ; Vol. 2. pp. 1-108.

Bibtex

@article{224a0d8f51104ebf8911e7b0ea049a1e,
title = "Effect of acute exercise on patella tendon protein synthesis and gene expression",
abstract = "Evidence suggests that habitual loading can result in patellar tendon hypertrophy, especially at the proximal and distal parts of the patellar tendon. The underlying protein kinetic changes and its regulation remains controversial and human data, investigating this topic, are limited. The present study investigated how acute exercise affects growth factor production and collagen fractional synthetic rate in patellar tendon tissue from patients undergoing an anterior cruciate ligament reconstruction operation. The operation was performed by use of the bone-patellar tendon-bone method under spinal anesthesia. Twelve subjects were randomized to one of two groups: a control group or an exercise group (1-hr unilateral knee-extension 67{\%} of Wmax 24 hours before operation). Two hours before the anterior cruciate ligament operation a flooding-dose of L-[1-(13)C]proline was given. Tissue from the most proximal part of the patellar tendon was obtained during the operation. Tendon collagen fractional synthetic rate and mRNA concentrations of TGF-{\ss}-1, CTGF, and type I and III collagen were measured. CTGF and type I collagen expression were higher in the exercise group compared to the control group (p <0.05). Type III collagen expression (p = 0.11), TGF-{\ss}-1 expression (p = 0.34), and collagen fractional synthetic rate (p = 0.26) did not differ between groups. Although the expression of CTGF and type I collagen were higher, the patellar tendon collagen fractional synthetic rate was not correspondingly higher after exercise. The elevated CTGF expression in the exercise group indicates that the TGF-beta pathway could be an important link between mechanical loading and stimulation of tendon tissue type I collagen expression.",
author = "Kasper Dideriksen and Sindby, {Ann Kathrine Ryberg} and Michael Krogsgaard and Peter Schjerling and Lars Holm and Henning Langberg",
year = "2013",
month = "12",
doi = "10.1186/2193-1801-2-109",
language = "English",
volume = "2",
pages = "1--108",
journal = "SpringerPlus",
issn = "2193-1801",
publisher = "SpringerOpen",

}

RIS

TY - JOUR

T1 - Effect of acute exercise on patella tendon protein synthesis and gene expression

AU - Dideriksen, Kasper

AU - Sindby, Ann Kathrine Ryberg

AU - Krogsgaard, Michael

AU - Schjerling, Peter

AU - Holm, Lars

AU - Langberg, Henning

PY - 2013/12

Y1 - 2013/12

N2 - Evidence suggests that habitual loading can result in patellar tendon hypertrophy, especially at the proximal and distal parts of the patellar tendon. The underlying protein kinetic changes and its regulation remains controversial and human data, investigating this topic, are limited. The present study investigated how acute exercise affects growth factor production and collagen fractional synthetic rate in patellar tendon tissue from patients undergoing an anterior cruciate ligament reconstruction operation. The operation was performed by use of the bone-patellar tendon-bone method under spinal anesthesia. Twelve subjects were randomized to one of two groups: a control group or an exercise group (1-hr unilateral knee-extension 67% of Wmax 24 hours before operation). Two hours before the anterior cruciate ligament operation a flooding-dose of L-[1-(13)C]proline was given. Tissue from the most proximal part of the patellar tendon was obtained during the operation. Tendon collagen fractional synthetic rate and mRNA concentrations of TGF-ß-1, CTGF, and type I and III collagen were measured. CTGF and type I collagen expression were higher in the exercise group compared to the control group (p <0.05). Type III collagen expression (p = 0.11), TGF-ß-1 expression (p = 0.34), and collagen fractional synthetic rate (p = 0.26) did not differ between groups. Although the expression of CTGF and type I collagen were higher, the patellar tendon collagen fractional synthetic rate was not correspondingly higher after exercise. The elevated CTGF expression in the exercise group indicates that the TGF-beta pathway could be an important link between mechanical loading and stimulation of tendon tissue type I collagen expression.

AB - Evidence suggests that habitual loading can result in patellar tendon hypertrophy, especially at the proximal and distal parts of the patellar tendon. The underlying protein kinetic changes and its regulation remains controversial and human data, investigating this topic, are limited. The present study investigated how acute exercise affects growth factor production and collagen fractional synthetic rate in patellar tendon tissue from patients undergoing an anterior cruciate ligament reconstruction operation. The operation was performed by use of the bone-patellar tendon-bone method under spinal anesthesia. Twelve subjects were randomized to one of two groups: a control group or an exercise group (1-hr unilateral knee-extension 67% of Wmax 24 hours before operation). Two hours before the anterior cruciate ligament operation a flooding-dose of L-[1-(13)C]proline was given. Tissue from the most proximal part of the patellar tendon was obtained during the operation. Tendon collagen fractional synthetic rate and mRNA concentrations of TGF-ß-1, CTGF, and type I and III collagen were measured. CTGF and type I collagen expression were higher in the exercise group compared to the control group (p <0.05). Type III collagen expression (p = 0.11), TGF-ß-1 expression (p = 0.34), and collagen fractional synthetic rate (p = 0.26) did not differ between groups. Although the expression of CTGF and type I collagen were higher, the patellar tendon collagen fractional synthetic rate was not correspondingly higher after exercise. The elevated CTGF expression in the exercise group indicates that the TGF-beta pathway could be an important link between mechanical loading and stimulation of tendon tissue type I collagen expression.

U2 - 10.1186/2193-1801-2-109

DO - 10.1186/2193-1801-2-109

M3 - Journal article

VL - 2

SP - 1

EP - 108

JO - SpringerPlus

JF - SpringerPlus

SN - 2193-1801

ER -

ID: 45258318