Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans. / Crameri, Regina M; Langberg, Henning; Teisner, Børge; Magnusson, Peter; Schrøder, Henrik Daa; Olesen, Jens L; Jensen, Charlotte H; Koskinen, Satu; Suetta, Charlotte; Kjaer, Michael.

In: Matrix Biology, Vol. 23, No. 4, 2004, p. 259-64.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Crameri, RM, Langberg, H, Teisner, B, Magnusson, P, Schrøder, HD, Olesen, JL, Jensen, CH, Koskinen, S, Suetta, C & Kjaer, M 2004, 'Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans', Matrix Biology, vol. 23, no. 4, pp. 259-64. https://doi.org/10.1016/j.matbio.2004.05.009

APA

Crameri, R. M., Langberg, H., Teisner, B., Magnusson, P., Schrøder, H. D., Olesen, J. L., ... Kjaer, M. (2004). Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans. Matrix Biology, 23(4), 259-64. https://doi.org/10.1016/j.matbio.2004.05.009

Vancouver

Crameri RM, Langberg H, Teisner B, Magnusson P, Schrøder HD, Olesen JL et al. Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans. Matrix Biology. 2004;23(4):259-64. https://doi.org/10.1016/j.matbio.2004.05.009

Author

Crameri, Regina M ; Langberg, Henning ; Teisner, Børge ; Magnusson, Peter ; Schrøder, Henrik Daa ; Olesen, Jens L ; Jensen, Charlotte H ; Koskinen, Satu ; Suetta, Charlotte ; Kjaer, Michael. / Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans. In: Matrix Biology. 2004 ; Vol. 23, No. 4. pp. 259-64.

Bibtex

@article{9913a3b0a9614924afe2925f0a7b335e,
title = "Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans",
abstract = "Increases in procollagen processing within skeletal muscle have previously been reported in small animal models only. While indirect measurements in humans have suggested an increase procollagen processing, no intra-skeletal muscle measurements have confirmed these findings. In this study, eight young healthy male subjects performed a single bout of unaccustomed high intensity eccentric exercise on one leg, with the contralateral leg being the control. A significant increase in the muscle interstitial concentration of the N-terminal propeptide of procollagen type I (PINP) was observed (day 0: 1.96 +/- 0.44 ng ml(-1), day 2: 1.94 +/- 0.32 ng ml(-1), day 4: 3.90 +/- 1.03 ng ml(-1), day 8: 7.23 +/- 2.34 ng ml(-1)*, *P <0.05 vs. basal and control) with no change being noted in the control leg. By day 2 post-exercise, an increase in the histological immunoreactivity of PINP and the N-terminal propeptide of procollagen type III (PIIINP) was also shown in the exercising leg only. Further, from day 2 post-exercise, immunoreactivity for tenascin C and reactive macrophages (CD68+ cells) was seen within the perimysial and endomysial connective tissue of the exercising leg only, indicating a high mechanical load and inflammation. This study shows that following a single bout of high intensity eccentric exercise there is an increase in procollagen processing within skeletal muscle in humans.",
keywords = "Adult, Exercise, Extracellular Matrix Proteins, Humans, Leg, Macrophages, Male, Monocytes, Muscle, Skeletal, Peptide Fragments, Procollagen, Tenascin",
author = "Crameri, {Regina M} and Henning Langberg and B{\o}rge Teisner and Peter Magnusson and Schr{\o}der, {Henrik Daa} and Olesen, {Jens L} and Jensen, {Charlotte H} and Satu Koskinen and Charlotte Suetta and Michael Kjaer",
year = "2004",
doi = "10.1016/j.matbio.2004.05.009",
language = "English",
volume = "23",
pages = "259--64",
journal = "Matrix Biology",
issn = "0945-053X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Enhanced procollagen processing in skeletal muscle after a single bout of eccentric loading in humans

AU - Crameri, Regina M

AU - Langberg, Henning

AU - Teisner, Børge

AU - Magnusson, Peter

AU - Schrøder, Henrik Daa

AU - Olesen, Jens L

AU - Jensen, Charlotte H

AU - Koskinen, Satu

AU - Suetta, Charlotte

AU - Kjaer, Michael

PY - 2004

Y1 - 2004

N2 - Increases in procollagen processing within skeletal muscle have previously been reported in small animal models only. While indirect measurements in humans have suggested an increase procollagen processing, no intra-skeletal muscle measurements have confirmed these findings. In this study, eight young healthy male subjects performed a single bout of unaccustomed high intensity eccentric exercise on one leg, with the contralateral leg being the control. A significant increase in the muscle interstitial concentration of the N-terminal propeptide of procollagen type I (PINP) was observed (day 0: 1.96 +/- 0.44 ng ml(-1), day 2: 1.94 +/- 0.32 ng ml(-1), day 4: 3.90 +/- 1.03 ng ml(-1), day 8: 7.23 +/- 2.34 ng ml(-1)*, *P <0.05 vs. basal and control) with no change being noted in the control leg. By day 2 post-exercise, an increase in the histological immunoreactivity of PINP and the N-terminal propeptide of procollagen type III (PIIINP) was also shown in the exercising leg only. Further, from day 2 post-exercise, immunoreactivity for tenascin C and reactive macrophages (CD68+ cells) was seen within the perimysial and endomysial connective tissue of the exercising leg only, indicating a high mechanical load and inflammation. This study shows that following a single bout of high intensity eccentric exercise there is an increase in procollagen processing within skeletal muscle in humans.

AB - Increases in procollagen processing within skeletal muscle have previously been reported in small animal models only. While indirect measurements in humans have suggested an increase procollagen processing, no intra-skeletal muscle measurements have confirmed these findings. In this study, eight young healthy male subjects performed a single bout of unaccustomed high intensity eccentric exercise on one leg, with the contralateral leg being the control. A significant increase in the muscle interstitial concentration of the N-terminal propeptide of procollagen type I (PINP) was observed (day 0: 1.96 +/- 0.44 ng ml(-1), day 2: 1.94 +/- 0.32 ng ml(-1), day 4: 3.90 +/- 1.03 ng ml(-1), day 8: 7.23 +/- 2.34 ng ml(-1)*, *P <0.05 vs. basal and control) with no change being noted in the control leg. By day 2 post-exercise, an increase in the histological immunoreactivity of PINP and the N-terminal propeptide of procollagen type III (PIIINP) was also shown in the exercising leg only. Further, from day 2 post-exercise, immunoreactivity for tenascin C and reactive macrophages (CD68+ cells) was seen within the perimysial and endomysial connective tissue of the exercising leg only, indicating a high mechanical load and inflammation. This study shows that following a single bout of high intensity eccentric exercise there is an increase in procollagen processing within skeletal muscle in humans.

KW - Adult

KW - Exercise

KW - Extracellular Matrix Proteins

KW - Humans

KW - Leg

KW - Macrophages

KW - Male

KW - Monocytes

KW - Muscle, Skeletal

KW - Peptide Fragments

KW - Procollagen

KW - Tenascin

U2 - 10.1016/j.matbio.2004.05.009

DO - 10.1016/j.matbio.2004.05.009

M3 - Journal article

VL - 23

SP - 259

EP - 264

JO - Matrix Biology

JF - Matrix Biology

SN - 0945-053X

IS - 4

ER -

ID: 38366948