Inhibition of nitric oxide synthesis by systemic N(G)-monomethyl-L-arginine administration in humans: effects on interstitial adenosine, prostacyclin and potassium concentrations in resting and contracting skeletal muscle

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We examined whether the formation or the release of the vasodilators adenosine, prostacyclin (PGI(2)) and potassium (K(+)) increase in skeletal muscle interstitium in response to nitric oxide synthase (NOS) inhibition. Five subjects performed one-legged knee extensor exercise at 30 W without (controls) and with prior N(G)-nitro-L-arginine methyl ester (L-NAME) infusion (4 mg/kg, intravenously). Samples from the interstitial fluid were obtained at rest, during exercise and after exercise with the microdialysis technique. Interstitial adenosine in controls increased (p0.05) to controls. The 6-keto-prostaglandin F1alpha concentration in controls was 1.17+/-0.20 ng/ml at rest and increased (p0.05) in L-NAME. The interstitial K(+) concentration in controls increased (p
Original languageEnglish
JournalJournal of Vascular Research
Issue number4
Pages (from-to)297-302
Number of pages6
Publication statusPublished - 2000

    Research areas

  • 6-Ketoprostaglandin F1 alpha, Adenosine, Adult, Blood Pressure, Enzyme Inhibitors, Epoprostenol, Extracellular Space, Humans, Leg, Male, Muscle Contraction, Muscle, Skeletal, Nitric Oxide, Osmolar Concentration, Potassium, Regional Blood Flow, Rest, omega-N-Methylarginine

ID: 98570657