Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue. / Heinemeier, Katja; Langberg, Henning; Olesen, Jens L; Kjaer, Michael.

In: Journal of Applied Physiology, Vol. 95, No. 6, 2003, p. 2390-7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Heinemeier, K, Langberg, H, Olesen, JL & Kjaer, M 2003, 'Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue', Journal of Applied Physiology, vol. 95, no. 6, pp. 2390-7. https://doi.org/10.1152/japplphysiol.00403.2003

APA

Heinemeier, K., Langberg, H., Olesen, J. L., & Kjaer, M. (2003). Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue. Journal of Applied Physiology, 95(6), 2390-7. https://doi.org/10.1152/japplphysiol.00403.2003

Vancouver

Heinemeier K, Langberg H, Olesen JL, Kjaer M. Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue. Journal of Applied Physiology. 2003;95(6):2390-7. https://doi.org/10.1152/japplphysiol.00403.2003

Author

Heinemeier, Katja ; Langberg, Henning ; Olesen, Jens L ; Kjaer, Michael. / Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue. In: Journal of Applied Physiology. 2003 ; Vol. 95, No. 6. pp. 2390-7.

Bibtex

@article{dad31d2807834bc4ad5fa5a9fcb63c9c,
title = "Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue",
abstract = "Mechanical loading of tissue is known to influence local collagen synthesis, and microdialysis studies indicate that mechanical loading of human tendon during exercise elevates tendinous type I collagen production. Transforming growth factor-beta1 (TGF-beta1), a potent stimulator of type I collagen synthesis, is released from cultured tendon fibroblasts in response to mechanical loading. Thus TGF-beta1 could link mechanical loading and collagen synthesis in tendon tissue in vivo. Tissue levels of TGF-beta1 and type I collagen metabolism markers [procollagen I COOH-terminal propeptide (PICP) and COOH-terminal telopeptide of type I collagen (ICTP)] were measured by microdialysis in peritendinous tissue of the Achilles' tendon in six male volunteers before and after treadmill running (1 h, 12 km/h, 3{\%} uphill). In addition, blood levels of TGF-beta1, PICP, and ICTP were obtained. PICP levels increased 68 h after exercise (P <0.05). Dialysate levels of TGF-beta1 changed from 303 +/- 46 pg/ml (at rest) to 423 +/- 86 pg/ml 3 h postexercise. This change was nonsignificant, but the decay of tissue TGF-beta1 after catheter insertion was markedly delayed by exercise compared with the decay seen in resting subjects. Plasma concentrations of TGF-beta1 rose 30{\%} in response to exercise (P <0.05 vs. pre). Our observations indicate an increased local production of type I collagen in human peritendinous tissue in response to uphill running. Although not conclusive, changes in circulating and local TGF-beta1, in response to exercise, suggest a role for TGF-beta1 in mechanical regulation of local collagen type I synthesis in tendon-related connective tissue in vivo.",
keywords = "Achilles Tendon, Adult, Biological Markers, Collagen Type I, Enzyme-Linked Immunosorbent Assay, Exercise, Extracellular Matrix, Hematocrit, Humans, Male, Microdialysis, Tendons, Transforming Growth Factor beta",
author = "Katja Heinemeier and Henning Langberg and Olesen, {Jens L} and Michael Kjaer",
year = "2003",
doi = "10.1152/japplphysiol.00403.2003",
language = "English",
volume = "95",
pages = "2390--7",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "6",

}

RIS

TY - JOUR

T1 - Role of TGF-beta1 in relation to exercise-induced type I collagen synthesis in human tendinous tissue

AU - Heinemeier, Katja

AU - Langberg, Henning

AU - Olesen, Jens L

AU - Kjaer, Michael

PY - 2003

Y1 - 2003

N2 - Mechanical loading of tissue is known to influence local collagen synthesis, and microdialysis studies indicate that mechanical loading of human tendon during exercise elevates tendinous type I collagen production. Transforming growth factor-beta1 (TGF-beta1), a potent stimulator of type I collagen synthesis, is released from cultured tendon fibroblasts in response to mechanical loading. Thus TGF-beta1 could link mechanical loading and collagen synthesis in tendon tissue in vivo. Tissue levels of TGF-beta1 and type I collagen metabolism markers [procollagen I COOH-terminal propeptide (PICP) and COOH-terminal telopeptide of type I collagen (ICTP)] were measured by microdialysis in peritendinous tissue of the Achilles' tendon in six male volunteers before and after treadmill running (1 h, 12 km/h, 3% uphill). In addition, blood levels of TGF-beta1, PICP, and ICTP were obtained. PICP levels increased 68 h after exercise (P <0.05). Dialysate levels of TGF-beta1 changed from 303 +/- 46 pg/ml (at rest) to 423 +/- 86 pg/ml 3 h postexercise. This change was nonsignificant, but the decay of tissue TGF-beta1 after catheter insertion was markedly delayed by exercise compared with the decay seen in resting subjects. Plasma concentrations of TGF-beta1 rose 30% in response to exercise (P <0.05 vs. pre). Our observations indicate an increased local production of type I collagen in human peritendinous tissue in response to uphill running. Although not conclusive, changes in circulating and local TGF-beta1, in response to exercise, suggest a role for TGF-beta1 in mechanical regulation of local collagen type I synthesis in tendon-related connective tissue in vivo.

AB - Mechanical loading of tissue is known to influence local collagen synthesis, and microdialysis studies indicate that mechanical loading of human tendon during exercise elevates tendinous type I collagen production. Transforming growth factor-beta1 (TGF-beta1), a potent stimulator of type I collagen synthesis, is released from cultured tendon fibroblasts in response to mechanical loading. Thus TGF-beta1 could link mechanical loading and collagen synthesis in tendon tissue in vivo. Tissue levels of TGF-beta1 and type I collagen metabolism markers [procollagen I COOH-terminal propeptide (PICP) and COOH-terminal telopeptide of type I collagen (ICTP)] were measured by microdialysis in peritendinous tissue of the Achilles' tendon in six male volunteers before and after treadmill running (1 h, 12 km/h, 3% uphill). In addition, blood levels of TGF-beta1, PICP, and ICTP were obtained. PICP levels increased 68 h after exercise (P <0.05). Dialysate levels of TGF-beta1 changed from 303 +/- 46 pg/ml (at rest) to 423 +/- 86 pg/ml 3 h postexercise. This change was nonsignificant, but the decay of tissue TGF-beta1 after catheter insertion was markedly delayed by exercise compared with the decay seen in resting subjects. Plasma concentrations of TGF-beta1 rose 30% in response to exercise (P <0.05 vs. pre). Our observations indicate an increased local production of type I collagen in human peritendinous tissue in response to uphill running. Although not conclusive, changes in circulating and local TGF-beta1, in response to exercise, suggest a role for TGF-beta1 in mechanical regulation of local collagen type I synthesis in tendon-related connective tissue in vivo.

KW - Achilles Tendon

KW - Adult

KW - Biological Markers

KW - Collagen Type I

KW - Enzyme-Linked Immunosorbent Assay

KW - Exercise

KW - Extracellular Matrix

KW - Hematocrit

KW - Humans

KW - Male

KW - Microdialysis

KW - Tendons

KW - Transforming Growth Factor beta

U2 - 10.1152/japplphysiol.00403.2003

DO - 10.1152/japplphysiol.00403.2003

M3 - Journal article

VL - 95

SP - 2390

EP - 2397

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 6

ER -

ID: 38367709