Short-term strength training and the expression of myostatin and IGF-I isoforms in rat muscle and tendon: differential effects of specific contraction types

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K M Heinemeier, J L Olesen, P Schjerling, F Haddad, Henning Langberg, K M Baldwin, M Kjaer

In skeletal muscle, an increased expression of insulin like growth factor-I isoforms IGF-IEa and mechano-growth factor (MGF) combined with downregulation of myostatin is thought to be essential for training-induced hypertrophy. However, the specific effects of different contraction types on regulation of these factors in muscle are still unclear, and in tendon the functions of myostatin, IGF-IEa, and MGF in relation to training are unknown. Female Sprague-Dawley rats were subjected to 4 days of concentric, eccentric, or isometric training (n = 7-9 per group) of the medial gastrocnemius, by stimulation of the sciatic nerve during general anesthesia. mRNA levels for myostatin, IGF-IEa, and MGF in muscle and Achilles' tendon were measured by real-time RT-PCR. Muscle myostatin mRNA decreased in response to all types of training (2- to 8-fold) (P <0.05), but the effect of eccentric training was greater than concentric and isometric training (P <0.05). In tendon, myostatin mRNA was detected, but no changes were seen after exercise. IGF-IEa and MGF increased in muscle (up to 15-fold) and tendon (up to 4-fold) in response to training (P <0.01). In tendon no difference was seen between training types, but in muscle the effect of eccentric training was greater than concentric training for both IGF-IEa and MGF (P <0.05), and for IGF-IEa isometric training had greater effect than concentric (P <0.05). The results indicate a possible role for IGF-IEa and MGF in adaptation of tendon to training, and the combined changes in myostatin and IGF-IEa/MGF expression could explain the important effect of eccentric actions for muscle hypertrophy.
Original languageEnglish
JournalJournal of Applied Physiology
Volume102
Issue number2
Pages (from-to)573-81
Number of pages9
ISSN8750-7587
DOIs
Publication statusPublished - 2007

    Research areas

  • Animals, Female, Gene Expression Regulation, Hypertrophy, Insulin-Like Growth Factor I, Isometric Contraction, Muscle Contraction, Muscle, Skeletal, Myostatin, Physical Conditioning, Animal, Protein Isoforms, RNA, Messenger, Random Allocation, Rats, Rats, Sprague-Dawley, Tendons, Transforming Growth Factor beta

ID: 38366095