Biomarkers of DNA Oxidation Products: Links to Exposure and Disease in Public Health Studies

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Biomarkers of DNA Oxidation Products : Links to Exposure and Disease in Public Health Studies. / Møller, Peter; Roursgaard, Martin.

In: Chemical Research in Toxicology, Vol. 34, 2021, p. 2235−2250.

Research output: Contribution to journalReviewpeer-review

Harvard

Møller, P & Roursgaard, M 2021, 'Biomarkers of DNA Oxidation Products: Links to Exposure and Disease in Public Health Studies', Chemical Research in Toxicology, vol. 34, pp. 2235−2250. https://doi.org/10.1021/acs.chemrestox.1c00213

APA

Møller, P., & Roursgaard, M. (2021). Biomarkers of DNA Oxidation Products: Links to Exposure and Disease in Public Health Studies. Chemical Research in Toxicology, 34, 2235−2250. https://doi.org/10.1021/acs.chemrestox.1c00213

Vancouver

Møller P, Roursgaard M. Biomarkers of DNA Oxidation Products: Links to Exposure and Disease in Public Health Studies. Chemical Research in Toxicology. 2021;34:2235−2250. https://doi.org/10.1021/acs.chemrestox.1c00213

Author

Møller, Peter ; Roursgaard, Martin. / Biomarkers of DNA Oxidation Products : Links to Exposure and Disease in Public Health Studies. In: Chemical Research in Toxicology. 2021 ; Vol. 34. pp. 2235−2250.

Bibtex

@article{ee1b01f576f34e4497c43da2c2f52ca2,
title = "Biomarkers of DNA Oxidation Products: Links to Exposure and Disease in Public Health Studies",
abstract = "Environmental exposure can increase the production of reactive oxygen species and deplete cellular antioxidants in humans, resulting in oxidatively generated damage to DNA that is both a useful biomarker of oxidative stress and indicator of carcinogenic hazard. Methods of oxidatively damaged DNA analysis have been developed and used in public health research since the 1990s. Advanced techniques detect specific lesions, but they might not be applicable to complex matrixes (e.g., tissues), small sample volume, and large-scale studies. The most reliable methods are characterized by (1) detecting relevant DNA oxidation products (e.g., premutagenic lesions), (2) not harboring technical problems, (3) being applicable to complex biological mixtures, and (4) having the ability to process a large number of samples in a reasonable period of time. Most effort has been devoted to the measurements of 8-oxo-7,8-dihydro-2′-deoxyguanine (8-oxodG), which can be analyzed by chromatographic, enzymic, and antibody-based methods. Results from validation trials have shown that certain chromatographic and enzymic assays (namely the comet assay) are superior techniques. The enzyme-modified comet assay has been popular because it is technically simpler than chromatographic assays. It is widely used in public health studies on environmental exposures such as outdoor air pollution. Validated biomarker assays on oxidatively damaged DNA have been used to fill knowledge gaps between findings in prospective cohort studies and hazards from contemporary sources of air pollution exposures. Results from each of these research fields feed into public health research as approaches to conduct primary prevention of diseases caused by environmental or occupational agents. ",
author = "Peter M{\o}ller and Martin Roursgaard",
note = "Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",
year = "2021",
doi = "10.1021/acs.chemrestox.1c00213",
language = "English",
volume = "34",
pages = "2235−2250",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",

}

RIS

TY - JOUR

T1 - Biomarkers of DNA Oxidation Products

T2 - Links to Exposure and Disease in Public Health Studies

AU - Møller, Peter

AU - Roursgaard, Martin

N1 - Publisher Copyright: © 2021 American Chemical Society.

PY - 2021

Y1 - 2021

N2 - Environmental exposure can increase the production of reactive oxygen species and deplete cellular antioxidants in humans, resulting in oxidatively generated damage to DNA that is both a useful biomarker of oxidative stress and indicator of carcinogenic hazard. Methods of oxidatively damaged DNA analysis have been developed and used in public health research since the 1990s. Advanced techniques detect specific lesions, but they might not be applicable to complex matrixes (e.g., tissues), small sample volume, and large-scale studies. The most reliable methods are characterized by (1) detecting relevant DNA oxidation products (e.g., premutagenic lesions), (2) not harboring technical problems, (3) being applicable to complex biological mixtures, and (4) having the ability to process a large number of samples in a reasonable period of time. Most effort has been devoted to the measurements of 8-oxo-7,8-dihydro-2′-deoxyguanine (8-oxodG), which can be analyzed by chromatographic, enzymic, and antibody-based methods. Results from validation trials have shown that certain chromatographic and enzymic assays (namely the comet assay) are superior techniques. The enzyme-modified comet assay has been popular because it is technically simpler than chromatographic assays. It is widely used in public health studies on environmental exposures such as outdoor air pollution. Validated biomarker assays on oxidatively damaged DNA have been used to fill knowledge gaps between findings in prospective cohort studies and hazards from contemporary sources of air pollution exposures. Results from each of these research fields feed into public health research as approaches to conduct primary prevention of diseases caused by environmental or occupational agents.

AB - Environmental exposure can increase the production of reactive oxygen species and deplete cellular antioxidants in humans, resulting in oxidatively generated damage to DNA that is both a useful biomarker of oxidative stress and indicator of carcinogenic hazard. Methods of oxidatively damaged DNA analysis have been developed and used in public health research since the 1990s. Advanced techniques detect specific lesions, but they might not be applicable to complex matrixes (e.g., tissues), small sample volume, and large-scale studies. The most reliable methods are characterized by (1) detecting relevant DNA oxidation products (e.g., premutagenic lesions), (2) not harboring technical problems, (3) being applicable to complex biological mixtures, and (4) having the ability to process a large number of samples in a reasonable period of time. Most effort has been devoted to the measurements of 8-oxo-7,8-dihydro-2′-deoxyguanine (8-oxodG), which can be analyzed by chromatographic, enzymic, and antibody-based methods. Results from validation trials have shown that certain chromatographic and enzymic assays (namely the comet assay) are superior techniques. The enzyme-modified comet assay has been popular because it is technically simpler than chromatographic assays. It is widely used in public health studies on environmental exposures such as outdoor air pollution. Validated biomarker assays on oxidatively damaged DNA have been used to fill knowledge gaps between findings in prospective cohort studies and hazards from contemporary sources of air pollution exposures. Results from each of these research fields feed into public health research as approaches to conduct primary prevention of diseases caused by environmental or occupational agents.

U2 - 10.1021/acs.chemrestox.1c00213

DO - 10.1021/acs.chemrestox.1c00213

M3 - Review

C2 - 34704445

AN - SCOPUS:85118740207

VL - 34

SP - 2235−2250

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

ER -

ID: 285654761