Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex

Research output: Contribution to journalJournal articlepeer-review

Standard

Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex. / Skytt, Dorte Marie; Madsen, Karsten Kirkegaard; Pajecka, Kamilla; Schousboe, Arne; Waagepetersen, Helle S.

In: Neurochemical Research, Vol. 35, No. 12, 2010, p. 2043-2052.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Skytt, DM, Madsen, KK, Pajecka, K, Schousboe, A & Waagepetersen, HS 2010, 'Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex', Neurochemical Research, vol. 35, no. 12, pp. 2043-2052. https://doi.org/10.1007/s11064-010-0329-6

APA

Skytt, D. M., Madsen, K. K., Pajecka, K., Schousboe, A., & Waagepetersen, H. S. (2010). Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex. Neurochemical Research, 35(12), 2043-2052. https://doi.org/10.1007/s11064-010-0329-6

Vancouver

Skytt DM, Madsen KK, Pajecka K, Schousboe A, Waagepetersen HS. Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex. Neurochemical Research. 2010;35(12):2043-2052. https://doi.org/10.1007/s11064-010-0329-6

Author

Skytt, Dorte Marie ; Madsen, Karsten Kirkegaard ; Pajecka, Kamilla ; Schousboe, Arne ; Waagepetersen, Helle S. / Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex. In: Neurochemical Research. 2010 ; Vol. 35, No. 12. pp. 2043-2052.

Bibtex

@article{63e6a46bef1547cfac922fbf1a02ef5d,
title = "Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex",
abstract = "Astrocyte cultures were prepared from cerebral cortex of new-born and 7-day-old mice and additionally, the cultures from new-born animals were passaged as secondary cultures. The cultures were characterized by immunostaining for the astrocyte markers glutamine synthetase (GS), glial fibrillary acidic protein, and the glutamate transporters EAAT1 and EAAT2. The cultures prepared from 7-day-old animals were additionally characterized metabolically using (13)C-labeled glucose and glutamate as well as (15)N-labeled glutamate as substrates. All types of cultures exhibited pronounced immunostaining of the astrocyte marker proteins. The metabolic pattern of the cultures from 7-day-old animals of the labeled substrates was comparable to that seen previously in astrocyte cultures prepared from new-born mouse brain showing pronounced glycolytic and oxidative metabolism of glucose. Glutamate was metabolized both via the GS pathway and oxidatively via the tricarboxylic acid cycle as expected. Additionally, glutamate underwent pronounced transamination to aspartate and alanine and the intracellular pools of alanine and pyruvate exhibited compartmentation. Altogether the results show that cultures prepared from cerebral cortex of 7-day-old mice have metabolic and functional properties indistinguishable from those of classical astrocyte cultures prepared from neocortex of new-born animals. This provides flexibility with regard to preparation and use of these cultures for a variety of purposes.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Skytt, {Dorte Marie} and Madsen, {Karsten Kirkegaard} and Kamilla Pajecka and Arne Schousboe and Waagepetersen, {Helle S.}",
year = "2010",
doi = "10.1007/s11064-010-0329-6",
language = "English",
volume = "35",
pages = "2043--2052",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer",
number = "12",

}

RIS

TY - JOUR

T1 - Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex

AU - Skytt, Dorte Marie

AU - Madsen, Karsten Kirkegaard

AU - Pajecka, Kamilla

AU - Schousboe, Arne

AU - Waagepetersen, Helle S.

PY - 2010

Y1 - 2010

N2 - Astrocyte cultures were prepared from cerebral cortex of new-born and 7-day-old mice and additionally, the cultures from new-born animals were passaged as secondary cultures. The cultures were characterized by immunostaining for the astrocyte markers glutamine synthetase (GS), glial fibrillary acidic protein, and the glutamate transporters EAAT1 and EAAT2. The cultures prepared from 7-day-old animals were additionally characterized metabolically using (13)C-labeled glucose and glutamate as well as (15)N-labeled glutamate as substrates. All types of cultures exhibited pronounced immunostaining of the astrocyte marker proteins. The metabolic pattern of the cultures from 7-day-old animals of the labeled substrates was comparable to that seen previously in astrocyte cultures prepared from new-born mouse brain showing pronounced glycolytic and oxidative metabolism of glucose. Glutamate was metabolized both via the GS pathway and oxidatively via the tricarboxylic acid cycle as expected. Additionally, glutamate underwent pronounced transamination to aspartate and alanine and the intracellular pools of alanine and pyruvate exhibited compartmentation. Altogether the results show that cultures prepared from cerebral cortex of 7-day-old mice have metabolic and functional properties indistinguishable from those of classical astrocyte cultures prepared from neocortex of new-born animals. This provides flexibility with regard to preparation and use of these cultures for a variety of purposes.

AB - Astrocyte cultures were prepared from cerebral cortex of new-born and 7-day-old mice and additionally, the cultures from new-born animals were passaged as secondary cultures. The cultures were characterized by immunostaining for the astrocyte markers glutamine synthetase (GS), glial fibrillary acidic protein, and the glutamate transporters EAAT1 and EAAT2. The cultures prepared from 7-day-old animals were additionally characterized metabolically using (13)C-labeled glucose and glutamate as well as (15)N-labeled glutamate as substrates. All types of cultures exhibited pronounced immunostaining of the astrocyte marker proteins. The metabolic pattern of the cultures from 7-day-old animals of the labeled substrates was comparable to that seen previously in astrocyte cultures prepared from new-born mouse brain showing pronounced glycolytic and oxidative metabolism of glucose. Glutamate was metabolized both via the GS pathway and oxidatively via the tricarboxylic acid cycle as expected. Additionally, glutamate underwent pronounced transamination to aspartate and alanine and the intracellular pools of alanine and pyruvate exhibited compartmentation. Altogether the results show that cultures prepared from cerebral cortex of 7-day-old mice have metabolic and functional properties indistinguishable from those of classical astrocyte cultures prepared from neocortex of new-born animals. This provides flexibility with regard to preparation and use of these cultures for a variety of purposes.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/s11064-010-0329-6

DO - 10.1007/s11064-010-0329-6

M3 - Journal article

C2 - 21127969

VL - 35

SP - 2043

EP - 2052

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 12

ER -

ID: 32634219