Comparative Genomics of Vibrio cholerae O1 Isolated from Cholera Patients in Bangladesh
Research output: Contribution to conference › Poster › Research
Standard
Comparative Genomics of Vibrio cholerae O1 Isolated from Cholera Patients in Bangladesh. / Hossain, Zenat Zebin; Leekitcharoenphon, Pimlapas; Dalsgaard, Anders; Sultana, Rokaia; Begum, Anowara ; Jensen, Peter Kjær Mackie; Hendriksen, Rene S.
2017. Poster session presented at International Conference On Genomics Nanotech & Bio engineering , Dhaka, Bangladesh.Research output: Contribution to conference › Poster › Research
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - CONF
T1 - Comparative Genomics of Vibrio cholerae O1 Isolated from Cholera Patients in Bangladesh
AU - Hossain, Zenat Zebin
AU - Leekitcharoenphon, Pimlapas
AU - Dalsgaard, Anders
AU - Sultana, Rokaia
AU - Begum, Anowara
AU - Jensen, Peter Kjær Mackie
AU - Hendriksen, Rene S.
PY - 2017/5
Y1 - 2017/5
N2 - AIM: Cholera remains an endemic disease in Bangladesh and recently, the severity of the disease has significantly increased in urban area since the emergence of the new variant of Vibrio cholerae O1 El Tor. In this study, Whole Genome Sequencing (WGS) was utilized to investigate the current genomic profile of V. cholerae O1 strains, isolated from symptomatic patients in the low-income urban area of Arichpur, Dhaka, Bangladesh. METHODS: During October 2015, three V. cholerae O1 strains (VC-1, 2 and 3) were isolated from rectal swabs of two patients living in households 588 m apart. One of the two patients was co-infected with two V. cholerae strains (VC-1 and VC-3). Major virulence factors, biotype and antimicrobial resistance genes were identified by WGS. A global phylogenetic tree was inferred using genome wide SNPs (Single Nucleotide Polymorphism) analysis. RESULTS: All the V. cholerae strains were El Tor variant of Vibrio cholerae O1 carrying ctxB1 (standard classical genotype). SNP based global phylogeny revealed that these isolates are strictly clonal and the closest neighboring genomes were epidemic clones of V. cholera O1 from Pakistan isolated in 2010. All strains harbored the integrase gene of the SXT element (intSXT), antimicrobial resistance genes for aminoglycosides, phenicol, sulphonamide, and trimethoprim except VC-1 that lacked sulphonamide resistance genes. The in silico MultiLocus Sequence Typing (MLST) revealed that these strains belonged to sequence type 69. CONCLUSION: The study provides knowledge on current genetic traits of clinical V. cholerae O1 circulating in urban household clusters of Bangladesh which may help in predicting emergence of new pandemic strains in Bangladesh.
AB - AIM: Cholera remains an endemic disease in Bangladesh and recently, the severity of the disease has significantly increased in urban area since the emergence of the new variant of Vibrio cholerae O1 El Tor. In this study, Whole Genome Sequencing (WGS) was utilized to investigate the current genomic profile of V. cholerae O1 strains, isolated from symptomatic patients in the low-income urban area of Arichpur, Dhaka, Bangladesh. METHODS: During October 2015, three V. cholerae O1 strains (VC-1, 2 and 3) were isolated from rectal swabs of two patients living in households 588 m apart. One of the two patients was co-infected with two V. cholerae strains (VC-1 and VC-3). Major virulence factors, biotype and antimicrobial resistance genes were identified by WGS. A global phylogenetic tree was inferred using genome wide SNPs (Single Nucleotide Polymorphism) analysis. RESULTS: All the V. cholerae strains were El Tor variant of Vibrio cholerae O1 carrying ctxB1 (standard classical genotype). SNP based global phylogeny revealed that these isolates are strictly clonal and the closest neighboring genomes were epidemic clones of V. cholera O1 from Pakistan isolated in 2010. All strains harbored the integrase gene of the SXT element (intSXT), antimicrobial resistance genes for aminoglycosides, phenicol, sulphonamide, and trimethoprim except VC-1 that lacked sulphonamide resistance genes. The in silico MultiLocus Sequence Typing (MLST) revealed that these strains belonged to sequence type 69. CONCLUSION: The study provides knowledge on current genetic traits of clinical V. cholerae O1 circulating in urban household clusters of Bangladesh which may help in predicting emergence of new pandemic strains in Bangladesh.
M3 - Poster
Y2 - 14 May 2017 through 16 May 2017
ER -
ID: 189700524