Crx broadly modulates the pineal transcriptome

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Crx broadly modulates the pineal transcriptome. / Rovsing, Louise; Clokie, Samuel; Bustos, Diego M; Rohde, Kristian; Coon, Steven L; Litman, Thomas; Rath, Martin F; Møller, Morten; Klein, David C.

In: Journal of Neurochemistry, Vol. 119, No. 2, 2011, p. 262-74.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rovsing, L, Clokie, S, Bustos, DM, Rohde, K, Coon, SL, Litman, T, Rath, MF, Møller, M & Klein, DC 2011, 'Crx broadly modulates the pineal transcriptome', Journal of Neurochemistry, vol. 119, no. 2, pp. 262-74. https://doi.org/10.1111/j.1471-4159.2011.07405.x

APA

Rovsing, L., Clokie, S., Bustos, D. M., Rohde, K., Coon, S. L., Litman, T., ... Klein, D. C. (2011). Crx broadly modulates the pineal transcriptome. Journal of Neurochemistry, 119(2), 262-74. https://doi.org/10.1111/j.1471-4159.2011.07405.x

Vancouver

Rovsing L, Clokie S, Bustos DM, Rohde K, Coon SL, Litman T et al. Crx broadly modulates the pineal transcriptome. Journal of Neurochemistry. 2011;119(2):262-74. https://doi.org/10.1111/j.1471-4159.2011.07405.x

Author

Rovsing, Louise ; Clokie, Samuel ; Bustos, Diego M ; Rohde, Kristian ; Coon, Steven L ; Litman, Thomas ; Rath, Martin F ; Møller, Morten ; Klein, David C. / Crx broadly modulates the pineal transcriptome. In: Journal of Neurochemistry. 2011 ; Vol. 119, No. 2. pp. 262-74.

Bibtex

@article{f5aeac7b3b1840d28fa4c17eba6664b8,
title = "Crx broadly modulates the pineal transcriptome",
abstract = "Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa et al. 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx-/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.",
keywords = "Faculty of Health and Medical Sciences, neurobiology, circadian rhythms, gene expression",
author = "Louise Rovsing and Samuel Clokie and Bustos, {Diego M} and Kristian Rohde and Coon, {Steven L} and Thomas Litman and Rath, {Martin F} and Morten M{\o}ller and Klein, {David C}",
note = "Journal of Neurochemistry {\circledC} 2011 International Society for Neurochemistry.",
year = "2011",
doi = "10.1111/j.1471-4159.2011.07405.x",
language = "English",
volume = "119",
pages = "262--74",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Crx broadly modulates the pineal transcriptome

AU - Rovsing, Louise

AU - Clokie, Samuel

AU - Bustos, Diego M

AU - Rohde, Kristian

AU - Coon, Steven L

AU - Litman, Thomas

AU - Rath, Martin F

AU - Møller, Morten

AU - Klein, David C

N1 - Journal of Neurochemistry © 2011 International Society for Neurochemistry.

PY - 2011

Y1 - 2011

N2 - Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa et al. 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx-/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.

AB - Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa et al. 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx-/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.

KW - Faculty of Health and Medical Sciences

KW - neurobiology, circadian rhythms, gene expression

U2 - 10.1111/j.1471-4159.2011.07405.x

DO - 10.1111/j.1471-4159.2011.07405.x

M3 - Journal article

C2 - 21797868

VL - 119

SP - 262

EP - 274

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 2

ER -

ID: 33855154