Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic

Research output: Contribution to journalJournal articleResearchpeer-review

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Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic. / Pedersen, Marie; Vinzents, Peter; Petersen, Joergen Holm; Kleinjans, Jos C S; Plas, Gina; Kirsch-Volders, Micheline; Dostál, Miroslav; Rössner, Pavel; Beskid, Olena; Sram, Radim J; Merlo, Domenico Franco; Knudsen, Lisbeth E.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 608, No. 2, 2006, p. 112-20.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Pedersen, M, Vinzents, P, Petersen, JH, Kleinjans, JCS, Plas, G, Kirsch-Volders, M, Dostál, M, Rössner, P, Beskid, O, Sram, RJ, Merlo, DF & Knudsen, LE 2006, 'Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 608, no. 2, pp. 112-20. https://doi.org/10.1016/j.mrgentox.2006.02.013

APA

Pedersen, M., Vinzents, P., Petersen, J. H., Kleinjans, J. C. S., Plas, G., Kirsch-Volders, M., Dostál, M., Rössner, P., Beskid, O., Sram, R. J., Merlo, D. F., & Knudsen, L. E. (2006). Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 608(2), 112-20. https://doi.org/10.1016/j.mrgentox.2006.02.013

Vancouver

Pedersen M, Vinzents P, Petersen JH, Kleinjans JCS, Plas G, Kirsch-Volders M et al. Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2006;608(2):112-20. https://doi.org/10.1016/j.mrgentox.2006.02.013

Author

Pedersen, Marie ; Vinzents, Peter ; Petersen, Joergen Holm ; Kleinjans, Jos C S ; Plas, Gina ; Kirsch-Volders, Micheline ; Dostál, Miroslav ; Rössner, Pavel ; Beskid, Olena ; Sram, Radim J ; Merlo, Domenico Franco ; Knudsen, Lisbeth E. / Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2006 ; Vol. 608, No. 2. pp. 112-20.

Bibtex

@article{39c0b4000cd211df825d000ea68e967b,
title = "Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic",
abstract = "A family pilot study was conducted in the Czech Republic to test the hypothesis that exposure to air pollution with particulate matter (PM) in children results in detectable effects indicated by a number of biomarkers of exposure and early effects. The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBLs) was analysed to assess the cytogenetic effects in children and mothers living in two different areas. From each area two groups of children from a total of 24 families (mean age: 6.0+/-0.6 and 9.0+/-1.2 years) in a total of 47 children and 19 mothers (mean age: 33.6+/-3.9 years) participated. Chromosome aberrations determined with fluorescence in situ hybridization (FISH) painting for chromosomes #1 and #4 were analysed in 39 children and 20 parents. Teplice, a mining district, in Northern Bohemia was selected for the analyses of the effects in a population exposed to high levels of air pollution, especially during winter, and compared with a population from the rural area of Prachatice in Southern Bohemia. Significant higher frequencies of MN were found in the younger children living in the Teplice area as compared with those living in the Prachatice area (7.0+/-2.3 per thousand versus 4.9+/-2.0 per thousand, p=0.04). Higher levels of MN were also measured in the older children and the mothers from the Teplice area (9.2+/-3.7 per thousand versus 6.6+/-4.4 per thousand) and (12.6+/-3.4 per thousand versus 10.1+/-4.0 per thousand). The increased MN frequency may be associated with elevated carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) concentration of the PM(2.5) measured in the ambient Teplice air, but other factors like genotoxic compounds from the diet or protective effect of micronutrients, which was not addressed in this pilot study, may also differ between the two areas. MN frequencies were found to increase with age in children. Lower MN frequency was found in boys as compared to girls. The result of the FISH analyses showed a low number of individuals with detectable levels of aberrations and no significant increases in genomic frequency of stable chromosome exchanges (F(G)/100) were found in children or parents from the Teplice area in comparison with those from the Prachatice area. The family pilot study indicates that MN is a valuable and sensitive biomarker for early biological effect in children and adults living in two different areas characterised with significant exposure differences in c-PAHs concentrations during winter.",
author = "Marie Pedersen and Peter Vinzents and Petersen, {Joergen Holm} and Kleinjans, {Jos C S} and Gina Plas and Micheline Kirsch-Volders and Miroslav Dost{\'a}l and Pavel R{\"o}ssner and Olena Beskid and Sram, {Radim J} and Merlo, {Domenico Franco} and Knudsen, {Lisbeth E.}",
note = "Keywords: Adult; Air Pollutants; Air Pollution; Child; Child, Preschool; Cytogenetics; Czech Republic; Environmental Exposure; Female; Humans; In Situ Hybridization, Fluorescence; Male; Micronuclei, Chromosome-Defective; Micronucleus Tests; Pilot Projects; Siblings",
year = "2006",
doi = "10.1016/j.mrgentox.2006.02.013",
language = "English",
volume = "608",
pages = "112--20",
journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "0027-5107",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Cytogenetic effects in children and mothers exposed to air pollution assessed by the frequency of micronuclei and fluorescence in situ hybridization (FISH): a family pilot study in the Czech Republic

AU - Pedersen, Marie

AU - Vinzents, Peter

AU - Petersen, Joergen Holm

AU - Kleinjans, Jos C S

AU - Plas, Gina

AU - Kirsch-Volders, Micheline

AU - Dostál, Miroslav

AU - Rössner, Pavel

AU - Beskid, Olena

AU - Sram, Radim J

AU - Merlo, Domenico Franco

AU - Knudsen, Lisbeth E.

N1 - Keywords: Adult; Air Pollutants; Air Pollution; Child; Child, Preschool; Cytogenetics; Czech Republic; Environmental Exposure; Female; Humans; In Situ Hybridization, Fluorescence; Male; Micronuclei, Chromosome-Defective; Micronucleus Tests; Pilot Projects; Siblings

PY - 2006

Y1 - 2006

N2 - A family pilot study was conducted in the Czech Republic to test the hypothesis that exposure to air pollution with particulate matter (PM) in children results in detectable effects indicated by a number of biomarkers of exposure and early effects. The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBLs) was analysed to assess the cytogenetic effects in children and mothers living in two different areas. From each area two groups of children from a total of 24 families (mean age: 6.0+/-0.6 and 9.0+/-1.2 years) in a total of 47 children and 19 mothers (mean age: 33.6+/-3.9 years) participated. Chromosome aberrations determined with fluorescence in situ hybridization (FISH) painting for chromosomes #1 and #4 were analysed in 39 children and 20 parents. Teplice, a mining district, in Northern Bohemia was selected for the analyses of the effects in a population exposed to high levels of air pollution, especially during winter, and compared with a population from the rural area of Prachatice in Southern Bohemia. Significant higher frequencies of MN were found in the younger children living in the Teplice area as compared with those living in the Prachatice area (7.0+/-2.3 per thousand versus 4.9+/-2.0 per thousand, p=0.04). Higher levels of MN were also measured in the older children and the mothers from the Teplice area (9.2+/-3.7 per thousand versus 6.6+/-4.4 per thousand) and (12.6+/-3.4 per thousand versus 10.1+/-4.0 per thousand). The increased MN frequency may be associated with elevated carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) concentration of the PM(2.5) measured in the ambient Teplice air, but other factors like genotoxic compounds from the diet or protective effect of micronutrients, which was not addressed in this pilot study, may also differ between the two areas. MN frequencies were found to increase with age in children. Lower MN frequency was found in boys as compared to girls. The result of the FISH analyses showed a low number of individuals with detectable levels of aberrations and no significant increases in genomic frequency of stable chromosome exchanges (F(G)/100) were found in children or parents from the Teplice area in comparison with those from the Prachatice area. The family pilot study indicates that MN is a valuable and sensitive biomarker for early biological effect in children and adults living in two different areas characterised with significant exposure differences in c-PAHs concentrations during winter.

AB - A family pilot study was conducted in the Czech Republic to test the hypothesis that exposure to air pollution with particulate matter (PM) in children results in detectable effects indicated by a number of biomarkers of exposure and early effects. The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBLs) was analysed to assess the cytogenetic effects in children and mothers living in two different areas. From each area two groups of children from a total of 24 families (mean age: 6.0+/-0.6 and 9.0+/-1.2 years) in a total of 47 children and 19 mothers (mean age: 33.6+/-3.9 years) participated. Chromosome aberrations determined with fluorescence in situ hybridization (FISH) painting for chromosomes #1 and #4 were analysed in 39 children and 20 parents. Teplice, a mining district, in Northern Bohemia was selected for the analyses of the effects in a population exposed to high levels of air pollution, especially during winter, and compared with a population from the rural area of Prachatice in Southern Bohemia. Significant higher frequencies of MN were found in the younger children living in the Teplice area as compared with those living in the Prachatice area (7.0+/-2.3 per thousand versus 4.9+/-2.0 per thousand, p=0.04). Higher levels of MN were also measured in the older children and the mothers from the Teplice area (9.2+/-3.7 per thousand versus 6.6+/-4.4 per thousand) and (12.6+/-3.4 per thousand versus 10.1+/-4.0 per thousand). The increased MN frequency may be associated with elevated carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) concentration of the PM(2.5) measured in the ambient Teplice air, but other factors like genotoxic compounds from the diet or protective effect of micronutrients, which was not addressed in this pilot study, may also differ between the two areas. MN frequencies were found to increase with age in children. Lower MN frequency was found in boys as compared to girls. The result of the FISH analyses showed a low number of individuals with detectable levels of aberrations and no significant increases in genomic frequency of stable chromosome exchanges (F(G)/100) were found in children or parents from the Teplice area in comparison with those from the Prachatice area. The family pilot study indicates that MN is a valuable and sensitive biomarker for early biological effect in children and adults living in two different areas characterised with significant exposure differences in c-PAHs concentrations during winter.

U2 - 10.1016/j.mrgentox.2006.02.013

DO - 10.1016/j.mrgentox.2006.02.013

M3 - Journal article

C2 - 16829164

VL - 608

SP - 112

EP - 120

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 2

ER -

ID: 17273337