Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes

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Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes. / Lindvall, Therese; Karlsson, Jenny; Holmdahl, Rikard; Andersson, Åsa Inga Maria.

In: Arthritis Research & Therapy, Vol. 11, No. 1, 2009, p. R10.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Lindvall, T, Karlsson, J, Holmdahl, R & Andersson, ÅIM 2009, 'Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes', Arthritis Research & Therapy, vol. 11, no. 1, pp. R10. <http://arthritis-research.com/content/11/1/R10>

APA

Lindvall, T., Karlsson, J., Holmdahl, R., & Andersson, Å. I. M. (2009). Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes. Arthritis Research & Therapy, 11(1), R10. http://arthritis-research.com/content/11/1/R10

Vancouver

Lindvall T, Karlsson J, Holmdahl R, Andersson ÅIM. Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes. Arthritis Research & Therapy. 2009;11(1):R10.

Author

Lindvall, Therese ; Karlsson, Jenny ; Holmdahl, Rikard ; Andersson, Åsa Inga Maria. / Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes. In: Arthritis Research & Therapy. 2009 ; Vol. 11, No. 1. pp. R10.

Bibtex

@article{bd505790fc3c11de825d000ea68e967b,
title = "Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes",
abstract = "ABSTRACT: INTRODUCTION: In a cross between the susceptible B10.RIII (H-2r) and resistant RIIIS/J (H-2r) mouse strains, a locus on mouse chromosome 5 (Eae39) was previously shown to control experimental autoimmune encephalomyelitis (EAE). Recently, quantitative trait loci (QTL), linked to disease in different experimental arthritis models were mapped to this region. The aim of the present study was to investigate whether genes within Eae39, in addition to experimental autoimmune encephalomyelitis , control development of collagen induced arthritis (CIA). METHODS: Collagen induced arthritis, induced by immunization with bovine type II collagen, was studied in Eae39 congenic- and sub-interval congenic mice. Antibody titers were investigated with ELISA. Gene-typing was performed by micro-satellite mapping and statistics was calculated by standard methods. RESULTS: Collagen induced arthritis experiments with Eae39 congenic- and sub-interval congenic mice, carrying RIIIS/J genes on the B10.RIII genetic background, revealed three loci within Eae39 that control disease and anti-collagen antibody titers. Two of the loci promoted disease and the third locus was protecting from collagen induced arthritis development. By further breeding of mice with small congenic fragments, we identified a 3.2 Megabasepair (Mbp) interval that regulates disease. CONCLUSIONS: Disease promoting- and protecting genes within the Eae39 locus on mouse chromosome 5, control susceptibility to collagen induced arthritis. A disease-protecting locus in the telomeric part of Eae39 results in lower anti-collagen antibody responses. The study shows the importance of breeding sub-congenic mouse strains to reveal genetic effects on complex diseases.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Therese Lindvall and Jenny Karlsson and Rikard Holmdahl and Andersson, {{\AA}sa Inga Maria}",
year = "2009",
language = "English",
volume = "11",
pages = "R10",
journal = "Arthritis Research & Therapy",
issn = "1478-6354",
publisher = "BioMed Central",
number = "1",

}

RIS

TY - JOUR

T1 - Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes

AU - Lindvall, Therese

AU - Karlsson, Jenny

AU - Holmdahl, Rikard

AU - Andersson, Åsa Inga Maria

PY - 2009

Y1 - 2009

N2 - ABSTRACT: INTRODUCTION: In a cross between the susceptible B10.RIII (H-2r) and resistant RIIIS/J (H-2r) mouse strains, a locus on mouse chromosome 5 (Eae39) was previously shown to control experimental autoimmune encephalomyelitis (EAE). Recently, quantitative trait loci (QTL), linked to disease in different experimental arthritis models were mapped to this region. The aim of the present study was to investigate whether genes within Eae39, in addition to experimental autoimmune encephalomyelitis , control development of collagen induced arthritis (CIA). METHODS: Collagen induced arthritis, induced by immunization with bovine type II collagen, was studied in Eae39 congenic- and sub-interval congenic mice. Antibody titers were investigated with ELISA. Gene-typing was performed by micro-satellite mapping and statistics was calculated by standard methods. RESULTS: Collagen induced arthritis experiments with Eae39 congenic- and sub-interval congenic mice, carrying RIIIS/J genes on the B10.RIII genetic background, revealed three loci within Eae39 that control disease and anti-collagen antibody titers. Two of the loci promoted disease and the third locus was protecting from collagen induced arthritis development. By further breeding of mice with small congenic fragments, we identified a 3.2 Megabasepair (Mbp) interval that regulates disease. CONCLUSIONS: Disease promoting- and protecting genes within the Eae39 locus on mouse chromosome 5, control susceptibility to collagen induced arthritis. A disease-protecting locus in the telomeric part of Eae39 results in lower anti-collagen antibody responses. The study shows the importance of breeding sub-congenic mouse strains to reveal genetic effects on complex diseases.

AB - ABSTRACT: INTRODUCTION: In a cross between the susceptible B10.RIII (H-2r) and resistant RIIIS/J (H-2r) mouse strains, a locus on mouse chromosome 5 (Eae39) was previously shown to control experimental autoimmune encephalomyelitis (EAE). Recently, quantitative trait loci (QTL), linked to disease in different experimental arthritis models were mapped to this region. The aim of the present study was to investigate whether genes within Eae39, in addition to experimental autoimmune encephalomyelitis , control development of collagen induced arthritis (CIA). METHODS: Collagen induced arthritis, induced by immunization with bovine type II collagen, was studied in Eae39 congenic- and sub-interval congenic mice. Antibody titers were investigated with ELISA. Gene-typing was performed by micro-satellite mapping and statistics was calculated by standard methods. RESULTS: Collagen induced arthritis experiments with Eae39 congenic- and sub-interval congenic mice, carrying RIIIS/J genes on the B10.RIII genetic background, revealed three loci within Eae39 that control disease and anti-collagen antibody titers. Two of the loci promoted disease and the third locus was protecting from collagen induced arthritis development. By further breeding of mice with small congenic fragments, we identified a 3.2 Megabasepair (Mbp) interval that regulates disease. CONCLUSIONS: Disease promoting- and protecting genes within the Eae39 locus on mouse chromosome 5, control susceptibility to collagen induced arthritis. A disease-protecting locus in the telomeric part of Eae39 results in lower anti-collagen antibody responses. The study shows the importance of breeding sub-congenic mouse strains to reveal genetic effects on complex diseases.

KW - Former Faculty of Pharmaceutical Sciences

M3 - Journal article

C2 - 19154607

VL - 11

SP - R10

JO - Arthritis Research & Therapy

JF - Arthritis Research & Therapy

SN - 1478-6354

IS - 1

ER -

ID: 16862620