Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil

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Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil. / Cranston, Imogen ; Teoh, Penelope J. ; baker, Sarah M. ; Sengupta, Mita Eva; Ensink, Jeroen J. H. .

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 110, No. 7, 07.2016, p. 400-407.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Cranston, I, Teoh, PJ, baker, SM, Sengupta, ME & Ensink, JJH 2016, 'Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 110, no. 7, pp. 400-407. https://doi.org/10.1093/trstmh/trw044

APA

Cranston, I., Teoh, P. J., baker, S. M., Sengupta, M. E., & Ensink, J. J. H. (2016). Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil. Transactions of the Royal Society of Tropical Medicine and Hygiene, 110(7), 400-407. https://doi.org/10.1093/trstmh/trw044

Vancouver

Cranston I, Teoh PJ, baker SM, Sengupta ME, Ensink JJH. Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2016 Jul;110(7):400-407. https://doi.org/10.1093/trstmh/trw044

Author

Cranston, Imogen ; Teoh, Penelope J. ; baker, Sarah M. ; Sengupta, Mita Eva ; Ensink, Jeroen J. H. . / Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 2016 ; Vol. 110, No. 7. pp. 400-407.

Bibtex

@article{555d78bbe3b84cd594e7a7ba878666b4,
title = "Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil",
abstract = "Background: Soil transmitted helminths (STH) continue to be associated with high burdens of disease, with an estimated 1.45 billion people infected with STH globally. The promotion and construction of latrines is considered the first barrier to prevent transmission of STH. The absence of a reliable method to extract STH ova from soil makes it challenging to examine whether the use of latrines may or may not have an effect on environmental contamination with ova. The present study evaluated the recovery rate of a method developed to extract STH ova from soil. Methods: The adapted centrifugation and flotation technique was applied to 15 soil types, which were seeded with Ascaris suum ova. Soil type, soil moisture content, soil texture and organic matter content were assessed for each soil sample. Results: The average ova recovery rate was 28.2{\%}, with the recovery rate of the method decreasing with increasing soil moisture content, particle size and organic matter content. The association between recovery rate and organic matter content was statistically significant. Conclusions: The present study identified a low recovery rate for an adapted centrifugation-flotation method, although this was similar to the recovery rate demonstrated by other methods developed for soil. Soil organic matter content was significantly associated with ova recovery rates",
keywords = "Faculty of Health and Medical Sciences, Ascaris suum, Centrifugation-flotation, Hygiene, Sanitation, Soil, STH",
author = "Imogen Cranston and Teoh, {Penelope J.} and baker, {Sarah M.} and Sengupta, {Mita Eva} and Ensink, {Jeroen J. H.}",
year = "2016",
month = "7",
doi = "10.1093/trstmh/trw044",
language = "English",
volume = "110",
pages = "400--407",
journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",
issn = "0035-9203",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil

AU - Cranston, Imogen

AU - Teoh, Penelope J.

AU - baker, Sarah M.

AU - Sengupta, Mita Eva

AU - Ensink, Jeroen J. H.

PY - 2016/7

Y1 - 2016/7

N2 - Background: Soil transmitted helminths (STH) continue to be associated with high burdens of disease, with an estimated 1.45 billion people infected with STH globally. The promotion and construction of latrines is considered the first barrier to prevent transmission of STH. The absence of a reliable method to extract STH ova from soil makes it challenging to examine whether the use of latrines may or may not have an effect on environmental contamination with ova. The present study evaluated the recovery rate of a method developed to extract STH ova from soil. Methods: The adapted centrifugation and flotation technique was applied to 15 soil types, which were seeded with Ascaris suum ova. Soil type, soil moisture content, soil texture and organic matter content were assessed for each soil sample. Results: The average ova recovery rate was 28.2%, with the recovery rate of the method decreasing with increasing soil moisture content, particle size and organic matter content. The association between recovery rate and organic matter content was statistically significant. Conclusions: The present study identified a low recovery rate for an adapted centrifugation-flotation method, although this was similar to the recovery rate demonstrated by other methods developed for soil. Soil organic matter content was significantly associated with ova recovery rates

AB - Background: Soil transmitted helminths (STH) continue to be associated with high burdens of disease, with an estimated 1.45 billion people infected with STH globally. The promotion and construction of latrines is considered the first barrier to prevent transmission of STH. The absence of a reliable method to extract STH ova from soil makes it challenging to examine whether the use of latrines may or may not have an effect on environmental contamination with ova. The present study evaluated the recovery rate of a method developed to extract STH ova from soil. Methods: The adapted centrifugation and flotation technique was applied to 15 soil types, which were seeded with Ascaris suum ova. Soil type, soil moisture content, soil texture and organic matter content were assessed for each soil sample. Results: The average ova recovery rate was 28.2%, with the recovery rate of the method decreasing with increasing soil moisture content, particle size and organic matter content. The association between recovery rate and organic matter content was statistically significant. Conclusions: The present study identified a low recovery rate for an adapted centrifugation-flotation method, although this was similar to the recovery rate demonstrated by other methods developed for soil. Soil organic matter content was significantly associated with ova recovery rates

KW - Faculty of Health and Medical Sciences

KW - Ascaris suum

KW - Centrifugation-flotation

KW - Hygiene

KW - Sanitation

KW - Soil

KW - STH

U2 - 10.1093/trstmh/trw044

DO - 10.1093/trstmh/trw044

M3 - Journal article

C2 - 27496514

VL - 110

SP - 400

EP - 407

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

SN - 0035-9203

IS - 7

ER -

ID: 165396208