Expression, content, and localization of insulin-like growth factor I in human achilles tendon

Research output: Contribution to journal › Journal article › Research › peer-review

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Expression, content, and localization of insulin-like growth factor I in human achilles tendon. / Olesen, Jens L; Heinemeier, Katja M; Langberg, Henning; Magnusson, S Peter; Kjaer, Michael; Flyvbjerg, Allan.

In: Connective Tissue Research, Vol. 47, No. 4, 2006, p. 200-206.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olesen, JL, Heinemeier, KM, Langberg, H, Magnusson, SP, Kjaer, M & Flyvbjerg, A 2006, 'Expression, content, and localization of insulin-like growth factor I in human achilles tendon', Connective Tissue Research, vol. 47, no. 4, pp. 200-206. https://doi.org/10.1080/03008200600809893

APA

Olesen, J. L., Heinemeier, K. M., Langberg, H., Magnusson, S. P., Kjaer, M., & Flyvbjerg, A. (2006). Expression, content, and localization of insulin-like growth factor I in human achilles tendon. Connective Tissue Research, 47(4), 200-206. https://doi.org/10.1080/03008200600809893

Vancouver

Olesen JL, Heinemeier KM, Langberg H, Magnusson SP, Kjaer M, Flyvbjerg A. Expression, content, and localization of insulin-like growth factor I in human achilles tendon. Connective Tissue Research. 2006;47(4):200-206. https://doi.org/10.1080/03008200600809893

Author

Olesen, Jens L ; Heinemeier, Katja M ; Langberg, Henning ; Magnusson, S Peter ; Kjaer, Michael ; Flyvbjerg, Allan. / Expression, content, and localization of insulin-like growth factor I in human achilles tendon. In: Connective Tissue Research. 2006 ; Vol. 47, No. 4. pp. 200-206.

Bibtex

@article{ff0326ea66bf42b2b3b50dfeb7ea1d04,
title = "Expression, content, and localization of insulin-like growth factor I in human achilles tendon",
abstract = "In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.",
keywords = "Achilles Tendon, Adult, Fluorescent Antibody Technique, Indirect, Gene Expression, Humans, Insulin-Like Growth Factor Binding Protein 4, Insulin-Like Growth Factor I, Male, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction",
author = "Olesen, {Jens L} and Heinemeier, {Katja M} and Henning Langberg and Magnusson, {S Peter} and Michael Kjaer and Allan Flyvbjerg",
year = "2006",
doi = "10.1080/03008200600809893",
language = "English",
volume = "47",
pages = "200--206",
journal = "Connective Tissue Research",
issn = "0300-8207",
publisher = "Taylor & Francis",
number = "4",

}

RIS

TY - JOUR

T1 - Expression, content, and localization of insulin-like growth factor I in human achilles tendon

AU - Olesen, Jens L

AU - Heinemeier, Katja M

AU - Langberg, Henning

AU - Magnusson, S Peter

AU - Kjaer, Michael

AU - Flyvbjerg, Allan

PY - 2006

Y1 - 2006

N2 - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.

AB - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.

KW - Achilles Tendon

KW - Adult

KW - Fluorescent Antibody Technique, Indirect

KW - Gene Expression

KW - Humans

KW - Insulin-Like Growth Factor Binding Protein 4

KW - Insulin-Like Growth Factor I

KW - Male

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1080/03008200600809893

DO - 10.1080/03008200600809893

M3 - Journal article

C2 - 16987751

VL - 47

SP - 200

EP - 206

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

IS - 4

ER -

ID: 38366164