FLIPR assays of intracellular calcium in GPCR drug discovery

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Standard

FLIPR assays of intracellular calcium in GPCR drug discovery. / Hansen, Kasper Bø; Bräuner-Osborne, Hans.

G protein-coupled receptors in drug discovery. ed. / Wayne R Leifert. Vol. 552 United States : Humana Press, 2009. p. 269-278 (Methods in Molecular Biology, Vol. 552).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Harvard

Hansen, KB & Bräuner-Osborne, H 2009, FLIPR assays of intracellular calcium in GPCR drug discovery. in WR Leifert (ed.), G protein-coupled receptors in drug discovery. vol. 552, Humana Press, United States, Methods in Molecular Biology, vol. 552, pp. 269-278. https://doi.org/10.1007/978-1-60327-317-6_19

APA

Hansen, K. B., & Bräuner-Osborne, H. (2009). FLIPR assays of intracellular calcium in GPCR drug discovery. In W. R. Leifert (Ed.), G protein-coupled receptors in drug discovery (Vol. 552, pp. 269-278). Humana Press. Methods in Molecular Biology Vol. 552 https://doi.org/10.1007/978-1-60327-317-6_19

Vancouver

Hansen KB, Bräuner-Osborne H. FLIPR assays of intracellular calcium in GPCR drug discovery. In Leifert WR, editor, G protein-coupled receptors in drug discovery. Vol. 552. United States: Humana Press. 2009. p. 269-278. (Methods in Molecular Biology, Vol. 552). https://doi.org/10.1007/978-1-60327-317-6_19

Author

Hansen, Kasper Bø ; Bräuner-Osborne, Hans. / FLIPR assays of intracellular calcium in GPCR drug discovery. G protein-coupled receptors in drug discovery. editor / Wayne R Leifert. Vol. 552 United States : Humana Press, 2009. pp. 269-278 (Methods in Molecular Biology, Vol. 552).

Bibtex

@inbook{62c547106c6b11de8bc9000ea68e967b,
title = "FLIPR assays of intracellular calcium in GPCR drug discovery",
abstract = "Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Hansen, {Kasper B{\o}} and Hans Br{\"a}uner-Osborne",
note = "Keywords: Intracellular calcium measurement, G protein-coupled receptors, probenecid, FLIPR, FlexStation, NOVO star. Chapter 19",
year = "2009",
doi = "10.1007/978-1-60327-317-6_19",
language = "English",
isbn = "978-1-60327-316-9",
volume = "552",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "269--278",
editor = "Leifert, {Wayne R}",
booktitle = "G protein-coupled receptors in drug discovery",
address = "United States",

}

RIS

TY - CHAP

T1 - FLIPR assays of intracellular calcium in GPCR drug discovery

AU - Hansen, Kasper Bø

AU - Bräuner-Osborne, Hans

N1 - Keywords: Intracellular calcium measurement, G protein-coupled receptors, probenecid, FLIPR, FlexStation, NOVO star. Chapter 19

PY - 2009

Y1 - 2009

N2 - Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.

AB - Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/978-1-60327-317-6_19

DO - 10.1007/978-1-60327-317-6_19

M3 - Book chapter

C2 - 19513656

SN - 978-1-60327-316-9

VL - 552

T3 - Methods in Molecular Biology

SP - 269

EP - 278

BT - G protein-coupled receptors in drug discovery

A2 - Leifert, Wayne R

PB - Humana Press

CY - United States

ER -

ID: 13063072