Standard
Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. / Barregard, Lars; Møller, Peter; Henriksen, Trine; Mistry, Vilas; Koppen, Gudrun; Rossner, Pavel; Sram, Radim J; Weimann, Allan; Poulsen, Henrik E; Nataf, Robert; Andreoli, Roberta; Manini, Paola; Marczylo, Tim; Lam, Patricia; Evans, Mark D; Kasai, Hiroshi; Kawai, Kazuaki; Li, Yun-Shan; Sakai, Kazuo; Singh, Rajinder; Teichert, Friederike; Farmer, Peter B; Rozalski, Rafal; Gackowski, Daniel; Siomek, Agnieszka; Saez, Guillermo T; Cerda, Concha; Broberg, Karin; Lindh, Christian; Hossain, Mohammad Bakhtiar; Haghdoost, Siamak; Hu, Chiung-Wen; Chao, Mu-Rong; Wu, Kuen-Yuh; Orhan, Hilmi; Senduran, Nilufer; Smith, Raymond J; Santella, Regina M; Su, Yali; Cortez, Czarina; Yeh, Susan; Olinski, Ryszard; Loft, Steffen; Cooke, Marcus S.
In:
Antioxidants & Redox Signaling, Vol. 18, No. 18, 20.06.2013, p. 2377-91.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Barregard, L
, Møller, P, Henriksen, T, Mistry, V, Koppen, G, Rossner, P, Sram, RJ, Weimann, A
, Poulsen, HE, Nataf, R, Andreoli, R, Manini, P, Marczylo, T, Lam, P, Evans, MD, Kasai, H, Kawai, K, Li, Y-S, Sakai, K, Singh, R, Teichert, F, Farmer, PB, Rozalski, R, Gackowski, D, Siomek, A, Saez, GT, Cerda, C, Broberg, K, Lindh, C, Hossain, MB, Haghdoost, S, Hu, C-W, Chao, M-R, Wu, K-Y, Orhan, H, Senduran, N, Smith, RJ, Santella, RM, Su, Y, Cortez, C, Yeh, S, Olinski, R
, Loft, S & Cooke, MS 2013, '
Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine',
Antioxidants & Redox Signaling, vol. 18, no. 18, pp. 2377-91.
https://doi.org/10.1089/ars.2012.4714APA
Barregard, L.
, Møller, P., Henriksen, T., Mistry, V., Koppen, G., Rossner, P., Sram, R. J., Weimann, A.
, Poulsen, H. E., Nataf, R., Andreoli, R., Manini, P., Marczylo, T., Lam, P., Evans, M. D., Kasai, H., Kawai, K., Li, Y-S., Sakai, K., ... Cooke, M. S. (2013).
Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.
Antioxidants & Redox Signaling,
18(18), 2377-91.
https://doi.org/10.1089/ars.2012.4714Vancouver
Barregard L
, Møller P, Henriksen T, Mistry V, Koppen G, Rossner P et al.
Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.
Antioxidants & Redox Signaling. 2013 Jun 20;18(18):2377-91.
https://doi.org/10.1089/ars.2012.4714Author
Barregard, Lars ; Møller, Peter ; Henriksen, Trine ; Mistry, Vilas ; Koppen, Gudrun ; Rossner, Pavel ; Sram, Radim J ; Weimann, Allan ; Poulsen, Henrik E ; Nataf, Robert ; Andreoli, Roberta ; Manini, Paola ; Marczylo, Tim ; Lam, Patricia ; Evans, Mark D ; Kasai, Hiroshi ; Kawai, Kazuaki ; Li, Yun-Shan ; Sakai, Kazuo ; Singh, Rajinder ; Teichert, Friederike ; Farmer, Peter B ; Rozalski, Rafal ; Gackowski, Daniel ; Siomek, Agnieszka ; Saez, Guillermo T ; Cerda, Concha ; Broberg, Karin ; Lindh, Christian ; Hossain, Mohammad Bakhtiar ; Haghdoost, Siamak ; Hu, Chiung-Wen ; Chao, Mu-Rong ; Wu, Kuen-Yuh ; Orhan, Hilmi ; Senduran, Nilufer ; Smith, Raymond J ; Santella, Regina M ; Su, Yali ; Cortez, Czarina ; Yeh, Susan ; Olinski, Ryszard ; Loft, Steffen ; Cooke, Marcus S. / Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine. In: Antioxidants & Redox Signaling. 2013 ; Vol. 18, No. 18. pp. 2377-91.
Bibtex
@article{0b029ba66f6b4bcdaaf07500390025ff,
title = "Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine",
abstract = "Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.",
keywords = "Adult, Artifacts, Buffers, Deoxyguanosine, Female, Head and Neck Neoplasms, Humans, Male, Middle Aged, Reference Standards, Reproducibility of Results, Sodium Chloride, Solutions, Urinalysis, Young Adult",
author = "Lars Barregard and Peter M{\o}ller and Trine Henriksen and Vilas Mistry and Gudrun Koppen and Pavel Rossner and Sram, {Radim J} and Allan Weimann and Poulsen, {Henrik E} and Robert Nataf and Roberta Andreoli and Paola Manini and Tim Marczylo and Patricia Lam and Evans, {Mark D} and Hiroshi Kasai and Kazuaki Kawai and Yun-Shan Li and Kazuo Sakai and Rajinder Singh and Friederike Teichert and Farmer, {Peter B} and Rafal Rozalski and Daniel Gackowski and Agnieszka Siomek and Saez, {Guillermo T} and Concha Cerda and Karin Broberg and Christian Lindh and Hossain, {Mohammad Bakhtiar} and Siamak Haghdoost and Chiung-Wen Hu and Mu-Rong Chao and Kuen-Yuh Wu and Hilmi Orhan and Nilufer Senduran and Smith, {Raymond J} and Santella, {Regina M} and Yali Su and Czarina Cortez and Susan Yeh and Ryszard Olinski and Steffen Loft and Cooke, {Marcus S}",
year = "2013",
month = jun,
day = "20",
doi = "10.1089/ars.2012.4714",
language = "English",
volume = "18",
pages = "2377--91",
journal = "Antioxidants and Redox Signaling",
issn = "1523-0864",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "18",
}
RIS
TY - JOUR
T1 - Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine
AU - Barregard, Lars
AU - Møller, Peter
AU - Henriksen, Trine
AU - Mistry, Vilas
AU - Koppen, Gudrun
AU - Rossner, Pavel
AU - Sram, Radim J
AU - Weimann, Allan
AU - Poulsen, Henrik E
AU - Nataf, Robert
AU - Andreoli, Roberta
AU - Manini, Paola
AU - Marczylo, Tim
AU - Lam, Patricia
AU - Evans, Mark D
AU - Kasai, Hiroshi
AU - Kawai, Kazuaki
AU - Li, Yun-Shan
AU - Sakai, Kazuo
AU - Singh, Rajinder
AU - Teichert, Friederike
AU - Farmer, Peter B
AU - Rozalski, Rafal
AU - Gackowski, Daniel
AU - Siomek, Agnieszka
AU - Saez, Guillermo T
AU - Cerda, Concha
AU - Broberg, Karin
AU - Lindh, Christian
AU - Hossain, Mohammad Bakhtiar
AU - Haghdoost, Siamak
AU - Hu, Chiung-Wen
AU - Chao, Mu-Rong
AU - Wu, Kuen-Yuh
AU - Orhan, Hilmi
AU - Senduran, Nilufer
AU - Smith, Raymond J
AU - Santella, Regina M
AU - Su, Yali
AU - Cortez, Czarina
AU - Yeh, Susan
AU - Olinski, Ryszard
AU - Loft, Steffen
AU - Cooke, Marcus S
PY - 2013/6/20
Y1 - 2013/6/20
N2 - Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.
AB - Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.
KW - Adult
KW - Artifacts
KW - Buffers
KW - Deoxyguanosine
KW - Female
KW - Head and Neck Neoplasms
KW - Humans
KW - Male
KW - Middle Aged
KW - Reference Standards
KW - Reproducibility of Results
KW - Sodium Chloride
KW - Solutions
KW - Urinalysis
KW - Young Adult
U2 - 10.1089/ars.2012.4714
DO - 10.1089/ars.2012.4714
M3 - Journal article
C2 - 23198723
VL - 18
SP - 2377
EP - 2391
JO - Antioxidants and Redox Signaling
JF - Antioxidants and Redox Signaling
SN - 1523-0864
IS - 18
ER -
