In vitro digestion testing of lipid-based delivery systems: calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products

Research output: Contribution to journalJournal articleResearchpeer-review

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In vitro digestion testing of lipid-based delivery systems : calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products. / Devraj, Ravi; Williams, Hywel D; Warren, Dallas B; Mullertz, Anette; Porter, Christopher J H; Pouton, Colin W.

In: International Journal of Pharmaceutics, Vol. 441, No. 1-2, 2012, p. 323-333.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Devraj, R, Williams, HD, Warren, DB, Mullertz, A, Porter, CJH & Pouton, CW 2012, 'In vitro digestion testing of lipid-based delivery systems: calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products', International Journal of Pharmaceutics, vol. 441, no. 1-2, pp. 323-333. https://doi.org/10.1016/j.ijpharm.2012.11.024

APA

Devraj, R., Williams, H. D., Warren, D. B., Mullertz, A., Porter, C. J. H., & Pouton, C. W. (2012). In vitro digestion testing of lipid-based delivery systems: calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products. International Journal of Pharmaceutics, 441(1-2), 323-333. https://doi.org/10.1016/j.ijpharm.2012.11.024

Vancouver

Devraj R, Williams HD, Warren DB, Mullertz A, Porter CJH, Pouton CW. In vitro digestion testing of lipid-based delivery systems: calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products. International Journal of Pharmaceutics. 2012;441(1-2):323-333. https://doi.org/10.1016/j.ijpharm.2012.11.024

Author

Devraj, Ravi ; Williams, Hywel D ; Warren, Dallas B ; Mullertz, Anette ; Porter, Christopher J H ; Pouton, Colin W. / In vitro digestion testing of lipid-based delivery systems : calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products. In: International Journal of Pharmaceutics. 2012 ; Vol. 441, No. 1-2. pp. 323-333.

Bibtex

@article{316333dbd9954135841f144597ed8378,
title = "In vitro digestion testing of lipid-based delivery systems: calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products",
abstract = "In vitro digestion testing is of practical importance to predict the fate of drugs administered in lipid-based delivery systems. Calcium ions are often added to digestion media to increase the extent of digestion of long-chain triglycerides (LCTs), but the effects they have on phase behaviour of the products of digestion, and consequent drug solubilization, are not well understood. This study investigates the effect of calcium and bile salt concentrations on the rate and extent of in vitro digestion of soybean oil, as well as the solubilizing capacity of the digestion products for two poorly water-soluble drugs, fenofibrate and danazol. In the presence of higher concentrations of calcium ions, the solubilization capacities of the digests were reduced for both drugs. This effect is attributed to the formation of insoluble calcium soaps, visible as precipitates during the digestions. This reduces the availability of liberated fatty acids to form mixed micelles and vesicles, thereby reducing drug solubilization. The use of high calcium concentrations does indeed force in vitro digestion of LCTs but may overestimate the extent of drug precipitation that occurs within the intestinal lumen.",
keywords = "The Faculty of Pharmaceutical Sciences",
author = "Ravi Devraj and Williams, {Hywel D} and Warren, {Dallas B} and Anette Mullertz and Porter, {Christopher J H} and Pouton, {Colin W}",
note = "Copyright {\circledC} 2012 Elsevier B.V. All rights reserved.",
year = "2012",
doi = "10.1016/j.ijpharm.2012.11.024",
language = "English",
volume = "441",
pages = "323--333",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - In vitro digestion testing of lipid-based delivery systems

T2 - calcium ions combine with fatty acids liberated from triglyceride rich lipid solutions to form soaps and reduce the solubilization capacity of colloidal digestion products

AU - Devraj, Ravi

AU - Williams, Hywel D

AU - Warren, Dallas B

AU - Mullertz, Anette

AU - Porter, Christopher J H

AU - Pouton, Colin W

N1 - Copyright © 2012 Elsevier B.V. All rights reserved.

PY - 2012

Y1 - 2012

N2 - In vitro digestion testing is of practical importance to predict the fate of drugs administered in lipid-based delivery systems. Calcium ions are often added to digestion media to increase the extent of digestion of long-chain triglycerides (LCTs), but the effects they have on phase behaviour of the products of digestion, and consequent drug solubilization, are not well understood. This study investigates the effect of calcium and bile salt concentrations on the rate and extent of in vitro digestion of soybean oil, as well as the solubilizing capacity of the digestion products for two poorly water-soluble drugs, fenofibrate and danazol. In the presence of higher concentrations of calcium ions, the solubilization capacities of the digests were reduced for both drugs. This effect is attributed to the formation of insoluble calcium soaps, visible as precipitates during the digestions. This reduces the availability of liberated fatty acids to form mixed micelles and vesicles, thereby reducing drug solubilization. The use of high calcium concentrations does indeed force in vitro digestion of LCTs but may overestimate the extent of drug precipitation that occurs within the intestinal lumen.

AB - In vitro digestion testing is of practical importance to predict the fate of drugs administered in lipid-based delivery systems. Calcium ions are often added to digestion media to increase the extent of digestion of long-chain triglycerides (LCTs), but the effects they have on phase behaviour of the products of digestion, and consequent drug solubilization, are not well understood. This study investigates the effect of calcium and bile salt concentrations on the rate and extent of in vitro digestion of soybean oil, as well as the solubilizing capacity of the digestion products for two poorly water-soluble drugs, fenofibrate and danazol. In the presence of higher concentrations of calcium ions, the solubilization capacities of the digests were reduced for both drugs. This effect is attributed to the formation of insoluble calcium soaps, visible as precipitates during the digestions. This reduces the availability of liberated fatty acids to form mixed micelles and vesicles, thereby reducing drug solubilization. The use of high calcium concentrations does indeed force in vitro digestion of LCTs but may overestimate the extent of drug precipitation that occurs within the intestinal lumen.

KW - The Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.ijpharm.2012.11.024

DO - 10.1016/j.ijpharm.2012.11.024

M3 - Journal article

VL - 441

SP - 323

EP - 333

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -

ID: 43972281