In vitro placental model optimization for nanoparticle transport studies

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

In vitro placental model optimization for nanoparticle transport studies. / Cartwright, Laura; Poulsen, Marie Sønnegaard; Nielsen, Hanne Mørck; Pojana, Giulio; Knudsen, Lisbeth E.; Saunders, Margaret; Rytting, Erik.

In: International Journal of Nanomedicine (Online), Vol. 7, 2012, p. 497-510.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Cartwright, L, Poulsen, MS, Nielsen, HM, Pojana, G, Knudsen, LE, Saunders, M & Rytting, E 2012, 'In vitro placental model optimization for nanoparticle transport studies', International Journal of Nanomedicine (Online), vol. 7, pp. 497-510. <http://dx.doi.org/10.2147/IJN.S26601>

APA

Cartwright, L., Poulsen, M. S., Nielsen, H. M., Pojana, G., Knudsen, L. E., Saunders, M., & Rytting, E. (2012). In vitro placental model optimization for nanoparticle transport studies. International Journal of Nanomedicine (Online), 7, 497-510. http://dx.doi.org/10.2147/IJN.S26601

Vancouver

Cartwright L, Poulsen MS, Nielsen HM, Pojana G, Knudsen LE, Saunders M et al. In vitro placental model optimization for nanoparticle transport studies. International Journal of Nanomedicine (Online). 2012;7:497-510.

Author

Cartwright, Laura ; Poulsen, Marie Sønnegaard ; Nielsen, Hanne Mørck ; Pojana, Giulio ; Knudsen, Lisbeth E. ; Saunders, Margaret ; Rytting, Erik. / In vitro placental model optimization for nanoparticle transport studies. In: International Journal of Nanomedicine (Online). 2012 ; Vol. 7. pp. 497-510.

Bibtex

@article{31cb2f50027c4518ac4d69cfa651f0da,
title = "In vitro placental model optimization for nanoparticle transport studies",
abstract = "Background: Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman{\textquoteright}s exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells. Methods: The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell{\textregistered} insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model. Results: Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 µm pore size) was four times higher for the 50 nm particles compared with the 100 nm particles. Conclusion: The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate. ",
keywords = "Faculty of Health and Medical Sciences, nanoparticles , placenta, BeWo cells, transport , model optimization, nanotoxicology",
author = "Laura Cartwright and Poulsen, {Marie S{\o}nnegaard} and Nielsen, {Hanne M{\o}rck} and Giulio Pojana and Knudsen, {Lisbeth E.} and Margaret Saunders and Erik Rytting",
year = "2012",
language = "English",
volume = "7",
pages = "497--510",
journal = "International Journal of Nanomedicine (Online)",
issn = "1176-9114",
publisher = "Dove Press Ltd",

}

RIS

TY - JOUR

T1 - In vitro placental model optimization for nanoparticle transport studies

AU - Cartwright, Laura

AU - Poulsen, Marie Sønnegaard

AU - Nielsen, Hanne Mørck

AU - Pojana, Giulio

AU - Knudsen, Lisbeth E.

AU - Saunders, Margaret

AU - Rytting, Erik

PY - 2012

Y1 - 2012

N2 - Background: Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman’s exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells. Methods: The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell® insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model. Results: Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 µm pore size) was four times higher for the 50 nm particles compared with the 100 nm particles. Conclusion: The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate.

AB - Background: Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman’s exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells. Methods: The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell® insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model. Results: Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 µm pore size) was four times higher for the 50 nm particles compared with the 100 nm particles. Conclusion: The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate.

KW - Faculty of Health and Medical Sciences

KW - nanoparticles

KW - placenta

KW - BeWo cells

KW - transport

KW - model optimization

KW - nanotoxicology

M3 - Journal article

VL - 7

SP - 497

EP - 510

JO - International Journal of Nanomedicine (Online)

JF - International Journal of Nanomedicine (Online)

SN - 1176-9114

ER -

ID: 37554440