Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

Research output: Contribution to journalJournal articlepeer-review

Standard

Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. / Mikkelsen, U R; Langberg, H; Helmark, I C; Skovgaard, D; Andersen, L L; Kjaer, M; Mackey, Abigail.

In: Journal of Applied Physiology, Vol. 107, No. 5, 11.2009, p. 1600-1611.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Mikkelsen, UR, Langberg, H, Helmark, IC, Skovgaard, D, Andersen, LL, Kjaer, M & Mackey, A 2009, 'Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise', Journal of Applied Physiology, vol. 107, no. 5, pp. 1600-1611. https://doi.org/10.1152/japplphysiol.00707.2009

APA

Mikkelsen, U. R., Langberg, H., Helmark, I. C., Skovgaard, D., Andersen, L. L., Kjaer, M., & Mackey, A. (2009). Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. Journal of Applied Physiology, 107(5), 1600-1611. https://doi.org/10.1152/japplphysiol.00707.2009

Vancouver

Mikkelsen UR, Langberg H, Helmark IC, Skovgaard D, Andersen LL, Kjaer M et al. Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. Journal of Applied Physiology. 2009 Nov;107(5):1600-1611. https://doi.org/10.1152/japplphysiol.00707.2009

Author

Mikkelsen, U R ; Langberg, H ; Helmark, I C ; Skovgaard, D ; Andersen, L L ; Kjaer, M ; Mackey, Abigail. / Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. In: Journal of Applied Physiology. 2009 ; Vol. 107, No. 5. pp. 1600-1611.

Bibtex

@article{efd719b38b984d5eb5600dae0590ef5c,
title = "Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise",
abstract = "Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.",
keywords = "Adult, Anti-Inflammatory Agents, Non-Steroidal, Cell Proliferation, Cytokines, Humans, Indomethacin, Infusions, Parenteral, Injections, Intramuscular, Male, Muscle, Skeletal, Physical Endurance, Physical Exertion, Satellite Cells, Skeletal Muscle, Young Adult",
author = "Mikkelsen, {U R} and H Langberg and Helmark, {I C} and D Skovgaard and Andersen, {L L} and M Kjaer and Abigail Mackey",
year = "2009",
month = nov,
doi = "10.1152/japplphysiol.00707.2009",
language = "English",
volume = "107",
pages = "1600--1611",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "5",

}

RIS

TY - JOUR

T1 - Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

AU - Mikkelsen, U R

AU - Langberg, H

AU - Helmark, I C

AU - Skovgaard, D

AU - Andersen, L L

AU - Kjaer, M

AU - Mackey, Abigail

PY - 2009/11

Y1 - 2009/11

N2 - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.

AB - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.

KW - Adult

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Cell Proliferation

KW - Cytokines

KW - Humans

KW - Indomethacin

KW - Infusions, Parenteral

KW - Injections, Intramuscular

KW - Male

KW - Muscle, Skeletal

KW - Physical Endurance

KW - Physical Exertion

KW - Satellite Cells, Skeletal Muscle

KW - Young Adult

U2 - 10.1152/japplphysiol.00707.2009

DO - 10.1152/japplphysiol.00707.2009

M3 - Journal article

C2 - 19713429

VL - 107

SP - 1600

EP - 1611

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 5

ER -

ID: 123222770