Measurement of skeletal muscle collagen breakdown by microdialysis
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Measurement of skeletal muscle collagen breakdown by microdialysis. / Miller, B F; Ellis, D; Robinson, M M; Rivera, J D; Kjaer, M; Langberg, Henning.
In: Scandinavian Journal of Medicine & Science in Sports, Vol. 21, No. 6, 2011, p. e1-e8.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Measurement of skeletal muscle collagen breakdown by microdialysis
AU - Miller, B F
AU - Ellis, D
AU - Robinson, M M
AU - Rivera, J D
AU - Kjaer, M
AU - Langberg, Henning
N1 - © 2010 John Wiley & Sons A/S.
PY - 2011
Y1 - 2011
N2 - Exercise increases the synthesis of collagen in the extracellular matrix of skeletal muscle. Breakdown of skeletal muscle collagen has not yet been determined because of technical limitations. The purpose of the present study was to use local sampling to determine skeletal muscle collagen breakdown. Microdialysis fibers were tested in vitro to predict bath hydroxyproline (OHP) concentrations. We used an N-methyl-N-[tert-butyldimethyl-silyl]trifluoroacetimide derivative to analyze OHP using gas chromatography–mass spectroscopy (GC–MS) and compared the results with a colorimetric OHP assay. Ten young, healthy male subjects performed a bout of resistance exercise with one leg, followed 17–21 h later by in vivo skeletal muscle sampling by microdialysis in exercised (EX) and control (CON) legs. Microdialysis reliably predicted [OHP] in vitro (R2=0.90). Analysis with GC–MS was strongly correlated to traditional analysis methods (CON: slope=1.03, R2=0.896, and P<0.05, EX: slope=0.795, R2=0.896, and P<0.05). We conclude that in vitro, microdialysis fibers were able to measure OHP concentrations and were sensitive to changes in concentrations, a strenuous bout of exercise did not increase skeletal muscle collagen breakdown 17–21 h post-exercise, and our measurement of OHP using GC–MS was in agreement with traditional assays.
AB - Exercise increases the synthesis of collagen in the extracellular matrix of skeletal muscle. Breakdown of skeletal muscle collagen has not yet been determined because of technical limitations. The purpose of the present study was to use local sampling to determine skeletal muscle collagen breakdown. Microdialysis fibers were tested in vitro to predict bath hydroxyproline (OHP) concentrations. We used an N-methyl-N-[tert-butyldimethyl-silyl]trifluoroacetimide derivative to analyze OHP using gas chromatography–mass spectroscopy (GC–MS) and compared the results with a colorimetric OHP assay. Ten young, healthy male subjects performed a bout of resistance exercise with one leg, followed 17–21 h later by in vivo skeletal muscle sampling by microdialysis in exercised (EX) and control (CON) legs. Microdialysis reliably predicted [OHP] in vitro (R2=0.90). Analysis with GC–MS was strongly correlated to traditional analysis methods (CON: slope=1.03, R2=0.896, and P<0.05, EX: slope=0.795, R2=0.896, and P<0.05). We conclude that in vitro, microdialysis fibers were able to measure OHP concentrations and were sensitive to changes in concentrations, a strenuous bout of exercise did not increase skeletal muscle collagen breakdown 17–21 h post-exercise, and our measurement of OHP using GC–MS was in agreement with traditional assays.
KW - Adolescent
KW - Adult
KW - Collagen
KW - Denmark
KW - Exercise Test
KW - Gas Chromatography-Mass Spectrometry
KW - Humans
KW - Hydroxyproline
KW - Male
KW - Microdialysis
KW - Muscle Fibers, Skeletal
KW - Young Adult
U2 - 10.1111/j.1600-0838.2010.01105.x
DO - 10.1111/j.1600-0838.2010.01105.x
M3 - Journal article
C2 - 20561272
VL - 21
SP - e1-e8
JO - Scandinavian Journal of Medicine & Science in Sports
JF - Scandinavian Journal of Medicine & Science in Sports
SN - 0905-7188
IS - 6
ER -
ID: 38364193