Measurement of skeletal muscle collagen breakdown by microdialysis

Research output: Contribution to journalJournal articlepeer-review

Standard

Measurement of skeletal muscle collagen breakdown by microdialysis. / Miller, B F; Ellis, D; Robinson, M M; Rivera, J D; Kjaer, M; Langberg, Henning.

In: Scandinavian Journal of Medicine & Science in Sports, Vol. 21, No. 6, 2011, p. e1-e8.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Miller, BF, Ellis, D, Robinson, MM, Rivera, JD, Kjaer, M & Langberg, H 2011, 'Measurement of skeletal muscle collagen breakdown by microdialysis', Scandinavian Journal of Medicine & Science in Sports, vol. 21, no. 6, pp. e1-e8. https://doi.org/10.1111/j.1600-0838.2010.01105.x

APA

Miller, B. F., Ellis, D., Robinson, M. M., Rivera, J. D., Kjaer, M., & Langberg, H. (2011). Measurement of skeletal muscle collagen breakdown by microdialysis. Scandinavian Journal of Medicine & Science in Sports, 21(6), e1-e8. https://doi.org/10.1111/j.1600-0838.2010.01105.x

Vancouver

Miller BF, Ellis D, Robinson MM, Rivera JD, Kjaer M, Langberg H. Measurement of skeletal muscle collagen breakdown by microdialysis. Scandinavian Journal of Medicine & Science in Sports. 2011;21(6):e1-e8. https://doi.org/10.1111/j.1600-0838.2010.01105.x

Author

Miller, B F ; Ellis, D ; Robinson, M M ; Rivera, J D ; Kjaer, M ; Langberg, Henning. / Measurement of skeletal muscle collagen breakdown by microdialysis. In: Scandinavian Journal of Medicine & Science in Sports. 2011 ; Vol. 21, No. 6. pp. e1-e8.

Bibtex

@article{fa338fc9f84e47ffb783e122c016ec52,
title = "Measurement of skeletal muscle collagen breakdown by microdialysis",
abstract = "Exercise increases the synthesis of collagen in the extracellular matrix of skeletal muscle. Breakdown of skeletal muscle collagen has not yet been determined because of technical limitations. The purpose of the present study was to use local sampling to determine skeletal muscle collagen breakdown. Microdialysis fibers were tested in vitro to predict bath hydroxyproline (OHP) concentrations. We used an N-methyl-N-[tert-butyldimethyl-silyl]trifluoroacetimide derivative to analyze OHP using gas chromatography–mass spectroscopy (GC–MS) and compared the results with a colorimetric OHP assay. Ten young, healthy male subjects performed a bout of resistance exercise with one leg, followed 17–21 h later by in vivo skeletal muscle sampling by microdialysis in exercised (EX) and control (CON) legs. Microdialysis reliably predicted [OHP] in vitro (R2=0.90). Analysis with GC–MS was strongly correlated to traditional analysis methods (CON: slope=1.03, R2=0.896, and P<0.05, EX: slope=0.795, R2=0.896, and P<0.05). We conclude that in vitro, microdialysis fibers were able to measure OHP concentrations and were sensitive to changes in concentrations, a strenuous bout of exercise did not increase skeletal muscle collagen breakdown 17–21 h post-exercise, and our measurement of OHP using GC–MS was in agreement with traditional assays. ",
keywords = "Adolescent, Adult, Collagen, Denmark, Exercise Test, Gas Chromatography-Mass Spectrometry, Humans, Hydroxyproline, Male, Microdialysis, Muscle Fibers, Skeletal, Young Adult",
author = "Miller, {B F} and D Ellis and Robinson, {M M} and Rivera, {J D} and M Kjaer and Henning Langberg",
note = "{\textcopyright} 2010 John Wiley & Sons A/S.",
year = "2011",
doi = "10.1111/j.1600-0838.2010.01105.x",
language = "English",
volume = "21",
pages = "e1--e8",
journal = "Scandinavian Journal of Medicine & Science in Sports",
issn = "0905-7188",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Measurement of skeletal muscle collagen breakdown by microdialysis

AU - Miller, B F

AU - Ellis, D

AU - Robinson, M M

AU - Rivera, J D

AU - Kjaer, M

AU - Langberg, Henning

N1 - © 2010 John Wiley & Sons A/S.

PY - 2011

Y1 - 2011

N2 - Exercise increases the synthesis of collagen in the extracellular matrix of skeletal muscle. Breakdown of skeletal muscle collagen has not yet been determined because of technical limitations. The purpose of the present study was to use local sampling to determine skeletal muscle collagen breakdown. Microdialysis fibers were tested in vitro to predict bath hydroxyproline (OHP) concentrations. We used an N-methyl-N-[tert-butyldimethyl-silyl]trifluoroacetimide derivative to analyze OHP using gas chromatography–mass spectroscopy (GC–MS) and compared the results with a colorimetric OHP assay. Ten young, healthy male subjects performed a bout of resistance exercise with one leg, followed 17–21 h later by in vivo skeletal muscle sampling by microdialysis in exercised (EX) and control (CON) legs. Microdialysis reliably predicted [OHP] in vitro (R2=0.90). Analysis with GC–MS was strongly correlated to traditional analysis methods (CON: slope=1.03, R2=0.896, and P<0.05, EX: slope=0.795, R2=0.896, and P<0.05). We conclude that in vitro, microdialysis fibers were able to measure OHP concentrations and were sensitive to changes in concentrations, a strenuous bout of exercise did not increase skeletal muscle collagen breakdown 17–21 h post-exercise, and our measurement of OHP using GC–MS was in agreement with traditional assays.

AB - Exercise increases the synthesis of collagen in the extracellular matrix of skeletal muscle. Breakdown of skeletal muscle collagen has not yet been determined because of technical limitations. The purpose of the present study was to use local sampling to determine skeletal muscle collagen breakdown. Microdialysis fibers were tested in vitro to predict bath hydroxyproline (OHP) concentrations. We used an N-methyl-N-[tert-butyldimethyl-silyl]trifluoroacetimide derivative to analyze OHP using gas chromatography–mass spectroscopy (GC–MS) and compared the results with a colorimetric OHP assay. Ten young, healthy male subjects performed a bout of resistance exercise with one leg, followed 17–21 h later by in vivo skeletal muscle sampling by microdialysis in exercised (EX) and control (CON) legs. Microdialysis reliably predicted [OHP] in vitro (R2=0.90). Analysis with GC–MS was strongly correlated to traditional analysis methods (CON: slope=1.03, R2=0.896, and P<0.05, EX: slope=0.795, R2=0.896, and P<0.05). We conclude that in vitro, microdialysis fibers were able to measure OHP concentrations and were sensitive to changes in concentrations, a strenuous bout of exercise did not increase skeletal muscle collagen breakdown 17–21 h post-exercise, and our measurement of OHP using GC–MS was in agreement with traditional assays.

KW - Adolescent

KW - Adult

KW - Collagen

KW - Denmark

KW - Exercise Test

KW - Gas Chromatography-Mass Spectrometry

KW - Humans

KW - Hydroxyproline

KW - Male

KW - Microdialysis

KW - Muscle Fibers, Skeletal

KW - Young Adult

U2 - 10.1111/j.1600-0838.2010.01105.x

DO - 10.1111/j.1600-0838.2010.01105.x

M3 - Journal article

C2 - 20561272

VL - 21

SP - e1-e8

JO - Scandinavian Journal of Medicine & Science in Sports

JF - Scandinavian Journal of Medicine & Science in Sports

SN - 0905-7188

IS - 6

ER -

ID: 38364193