Molecular Analysis and Toxigenic Potential of Vibrio cholerae Isolated from Hilsha fish (Tenualosa ilisha), Bangladesh
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Molecular Analysis and Toxigenic Potential of Vibrio cholerae Isolated from Hilsha fish (Tenualosa ilisha), Bangladesh. / Hossain, Zenat Zebin; Farhana, Israt; Tulsiani, Suhella; Jensen, Peter Kjær Mackie; Begum, Anowara .
2016. Poster session presented at American socierty for microbiology 2016, Bostom , United States.Research output: Contribution to conference › Poster › Research › peer-review
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T1 - Molecular Analysis and Toxigenic Potential of Vibrio cholerae Isolated from Hilsha fish (Tenualosa ilisha), Bangladesh
AU - Hossain, Zenat Zebin
AU - Farhana, Israt
AU - Tulsiani, Suhella
AU - Jensen, Peter Kjær Mackie
AU - Begum, Anowara
PY - 2016/6
Y1 - 2016/6
N2 - Exposure to contaminated fish may upsurge the virulent strains ofVibrio cholerae, the deadly human pathogen in the households ofrural and urban Bangladesh. Since V. cholerae spreading wasreported from the Bay of Bengal, this study hypothesized thatHilsha (Tenualosa ilisha), a marine and fresh water fish may serveas a transmission vehicle of potential emerging epidemic causingstrains. For this, we studied 9 toxigenic V. cholerae strains isolatedfrom Hilsha fish including 6 V. cholerae O1 and 3 non O1/O139serogroups for virulence associated genotype and their pathogenicpotential on animal model and human cancer cell line .The V. cholerae O1 and non- O1/O139 strains possessed diversevirulence genes but lacked some major toxin genes like ctxA, tcpetc. Eight of the nine strains showed survivability up to 1% sodiumchloride in broth culture which indicates their coastal origin. Allnine isolates were able to accumulate fluid in rabbit ileal loops. Cellfree culture supernatant of three O1 and two non O1 strainscaused distinctive cell death in established HeLa cell line. Diversepolymorphic patterns were revealed in Random amplifiedpolymorphic DNA (RAPD) fingerprinting except two non O1 isolates(I-49d and I-52a) showed considerable correlation in bandpatterns. Similar restriction fragment length polymorphism (RFLP)profile of groEL-I of V. cholerae chromosome 1 in all strainsindicated homogeneity in species level. Hierarchical cluster analysisby β subunit of RNA polymerase gene (rpoB) sequencing showedthat theses isolates did not cluster together and also distinct fromclinical and environmental toxigenic strains.Our results indicate the indigenous V. cholerae strains associatedwith Hilsha fish possesses considerable virulence potentialitydespite being quite diverse from current epidemic strains.
AB - Exposure to contaminated fish may upsurge the virulent strains ofVibrio cholerae, the deadly human pathogen in the households ofrural and urban Bangladesh. Since V. cholerae spreading wasreported from the Bay of Bengal, this study hypothesized thatHilsha (Tenualosa ilisha), a marine and fresh water fish may serveas a transmission vehicle of potential emerging epidemic causingstrains. For this, we studied 9 toxigenic V. cholerae strains isolatedfrom Hilsha fish including 6 V. cholerae O1 and 3 non O1/O139serogroups for virulence associated genotype and their pathogenicpotential on animal model and human cancer cell line .The V. cholerae O1 and non- O1/O139 strains possessed diversevirulence genes but lacked some major toxin genes like ctxA, tcpetc. Eight of the nine strains showed survivability up to 1% sodiumchloride in broth culture which indicates their coastal origin. Allnine isolates were able to accumulate fluid in rabbit ileal loops. Cellfree culture supernatant of three O1 and two non O1 strainscaused distinctive cell death in established HeLa cell line. Diversepolymorphic patterns were revealed in Random amplifiedpolymorphic DNA (RAPD) fingerprinting except two non O1 isolates(I-49d and I-52a) showed considerable correlation in bandpatterns. Similar restriction fragment length polymorphism (RFLP)profile of groEL-I of V. cholerae chromosome 1 in all strainsindicated homogeneity in species level. Hierarchical cluster analysisby β subunit of RNA polymerase gene (rpoB) sequencing showedthat theses isolates did not cluster together and also distinct fromclinical and environmental toxigenic strains.Our results indicate the indigenous V. cholerae strains associatedwith Hilsha fish possesses considerable virulence potentialitydespite being quite diverse from current epidemic strains.
UR - http://www.asm.org/index.php/asm-events/post-meeting-materials
M3 - Poster
T2 - American socierty for microbiology 2016
Y2 - 16 June 2016 through 20 June 2016
ER -
ID: 167359475