Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans

Research output: Contribution to journalJournal articlepeer-review

Standard

Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans. / Hansen, M; Trappe, T; Crameri, R M; Qvortrup, K; Kjaer, M; Langberg, H.

In: Scandinavian Journal of Medicine & Science in Sports Online, Vol. 19, No. 1, 2008, p. 75-82.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Hansen, M, Trappe, T, Crameri, RM, Qvortrup, K, Kjaer, M & Langberg, H 2008, 'Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans', Scandinavian Journal of Medicine & Science in Sports Online, vol. 19, no. 1, pp. 75-82. https://doi.org/10.1111/j.1600-0838.2007.00766.x

APA

Hansen, M., Trappe, T., Crameri, R. M., Qvortrup, K., Kjaer, M., & Langberg, H. (2008). Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans. Scandinavian Journal of Medicine & Science in Sports Online, 19(1), 75-82. https://doi.org/10.1111/j.1600-0838.2007.00766.x

Vancouver

Hansen M, Trappe T, Crameri RM, Qvortrup K, Kjaer M, Langberg H. Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans. Scandinavian Journal of Medicine & Science in Sports Online. 2008;19(1):75-82. https://doi.org/10.1111/j.1600-0838.2007.00766.x

Author

Hansen, M ; Trappe, T ; Crameri, R M ; Qvortrup, K ; Kjaer, M ; Langberg, H. / Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans. In: Scandinavian Journal of Medicine & Science in Sports Online. 2008 ; Vol. 19, No. 1. pp. 75-82.

Bibtex

@article{7304b560365611df8ed1000ea68e967b,
title = "Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans",
abstract = "Knowledge about the effects of exercise on myofibrillar protein breakdown in human subjects is limited. Our purpose was to measure the changes in the degradation of myofibrillar proteins in response to different ways of eliciting muscle contractions using the local interstitial 3-methyl-histidine (3-MH) concentration as a marker for myofibrillar protein breakdown. Untrained males (n=8, 22-27 years, range) performed 210 maximal isokinetic eccentric contractions with each leg on an isokinetic dynamometer. One leg performed voluntary (VOL) and the other leg performed electrically induced contractions (ES). Microdialysis probes were placed in m. vastus lateralis in both the legs immediately after, and 1 and 3 days post-exercise. Interstitial 3-MH was higher in ES vs VOL immediately after exercise (P<0.05). One and 3 days post-exercise no difference between the two exercise types was observed. Only after ES did the histochemical stainings show significant disruption of cytoskeletal proteins. Furthermore, intracellular disruption and destroyed Z-lines were markedly more pronounced in ES vs VOL. In conclusion, the local level of interstitial 3-MH in the skeletal muscle was significantly enhanced after ES compared with VOL immediately after exercise, while the level of 3-MH did not change in the post-exercise period after VOL. These results indicate that the local myofibrillar breakdown is accelerated after ES associated with severe myofiber damage.",
author = "M Hansen and T Trappe and Crameri, {R M} and K Qvortrup and M Kjaer and H Langberg",
note = "Keywords: Adult; Biological Markers; Denmark; Electric Stimulation; Exercise Test; Humans; Hydrolysis; Male; Methylhistidines; Muscle Contraction; Muscle Proteins; Myofibrils; Young Adult",
year = "2008",
doi = "10.1111/j.1600-0838.2007.00766.x",
language = "English",
volume = "19",
pages = "75--82",
journal = "Scandinavian Journal of Medicine & Science in Sports",
issn = "0905-7188",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Myofibrillar proteolysis in response to voluntary or electrically stimulated muscle contractions in humans

AU - Hansen, M

AU - Trappe, T

AU - Crameri, R M

AU - Qvortrup, K

AU - Kjaer, M

AU - Langberg, H

N1 - Keywords: Adult; Biological Markers; Denmark; Electric Stimulation; Exercise Test; Humans; Hydrolysis; Male; Methylhistidines; Muscle Contraction; Muscle Proteins; Myofibrils; Young Adult

PY - 2008

Y1 - 2008

N2 - Knowledge about the effects of exercise on myofibrillar protein breakdown in human subjects is limited. Our purpose was to measure the changes in the degradation of myofibrillar proteins in response to different ways of eliciting muscle contractions using the local interstitial 3-methyl-histidine (3-MH) concentration as a marker for myofibrillar protein breakdown. Untrained males (n=8, 22-27 years, range) performed 210 maximal isokinetic eccentric contractions with each leg on an isokinetic dynamometer. One leg performed voluntary (VOL) and the other leg performed electrically induced contractions (ES). Microdialysis probes were placed in m. vastus lateralis in both the legs immediately after, and 1 and 3 days post-exercise. Interstitial 3-MH was higher in ES vs VOL immediately after exercise (P<0.05). One and 3 days post-exercise no difference between the two exercise types was observed. Only after ES did the histochemical stainings show significant disruption of cytoskeletal proteins. Furthermore, intracellular disruption and destroyed Z-lines were markedly more pronounced in ES vs VOL. In conclusion, the local level of interstitial 3-MH in the skeletal muscle was significantly enhanced after ES compared with VOL immediately after exercise, while the level of 3-MH did not change in the post-exercise period after VOL. These results indicate that the local myofibrillar breakdown is accelerated after ES associated with severe myofiber damage.

AB - Knowledge about the effects of exercise on myofibrillar protein breakdown in human subjects is limited. Our purpose was to measure the changes in the degradation of myofibrillar proteins in response to different ways of eliciting muscle contractions using the local interstitial 3-methyl-histidine (3-MH) concentration as a marker for myofibrillar protein breakdown. Untrained males (n=8, 22-27 years, range) performed 210 maximal isokinetic eccentric contractions with each leg on an isokinetic dynamometer. One leg performed voluntary (VOL) and the other leg performed electrically induced contractions (ES). Microdialysis probes were placed in m. vastus lateralis in both the legs immediately after, and 1 and 3 days post-exercise. Interstitial 3-MH was higher in ES vs VOL immediately after exercise (P<0.05). One and 3 days post-exercise no difference between the two exercise types was observed. Only after ES did the histochemical stainings show significant disruption of cytoskeletal proteins. Furthermore, intracellular disruption and destroyed Z-lines were markedly more pronounced in ES vs VOL. In conclusion, the local level of interstitial 3-MH in the skeletal muscle was significantly enhanced after ES compared with VOL immediately after exercise, while the level of 3-MH did not change in the post-exercise period after VOL. These results indicate that the local myofibrillar breakdown is accelerated after ES associated with severe myofiber damage.

U2 - 10.1111/j.1600-0838.2007.00766.x

DO - 10.1111/j.1600-0838.2007.00766.x

M3 - Journal article

C2 - 18266789

VL - 19

SP - 75

EP - 82

JO - Scandinavian Journal of Medicine & Science in Sports

JF - Scandinavian Journal of Medicine & Science in Sports

SN - 0905-7188

IS - 1

ER -

ID: 18787159