Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs

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Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs. / Di Ianni, Emilio; Møller, Peter; Mortensen, Alicja; Szarek, Józef; Clausen, Per Axel; Saber, Anne Thoustrup; Vogel, Ulla; Jacobsen, Nicklas Raun.

In: Nanotoxicology, Vol. 14, No. 7, 2020, p. 869-892.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Di Ianni, E, Møller, P, Mortensen, A, Szarek, J, Clausen, PA, Saber, AT, Vogel, U & Jacobsen, NR 2020, 'Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs', Nanotoxicology, vol. 14, no. 7, pp. 869-892. https://doi.org/10.1080/17435390.2020.1771786

APA

Di Ianni, E., Møller, P., Mortensen, A., Szarek, J., Clausen, P. A., Saber, A. T., ... Jacobsen, N. R. (2020). Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs. Nanotoxicology, 14(7), 869-892. https://doi.org/10.1080/17435390.2020.1771786

Vancouver

Di Ianni E, Møller P, Mortensen A, Szarek J, Clausen PA, Saber AT et al. Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs. Nanotoxicology. 2020;14(7):869-892. https://doi.org/10.1080/17435390.2020.1771786

Author

Di Ianni, Emilio ; Møller, Peter ; Mortensen, Alicja ; Szarek, Józef ; Clausen, Per Axel ; Saber, Anne Thoustrup ; Vogel, Ulla ; Jacobsen, Nicklas Raun. / Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs. In: Nanotoxicology. 2020 ; Vol. 14, No. 7. pp. 869-892.

Bibtex

@article{926e0f1aab484d54b12fe6f7e22eab13,
title = "Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs",
abstract = "Surface modification by different quaternary ammonium compounds (QAC) makes nanoclays more compatible with various polymeric matrices, thereby expanding their potential applications. The growing industrial use of nanoclays could potentially pose a health risk for workers. Here, we assessed how surface modification of nanoclays modulates their pulmonary toxicity. An in vitro screening of the unmodified nanoclay Bentonite (montmorillonite) and four organomodified nanoclays (ONC); coated with various QAC, including benzalkonium chloride (BAC), guided the selection of the materials for the in vivo study. Mice were exposed via a single intratracheal instillation to 18, 54, and 162 µg of unmodified Bentonite or dialkyldimethyl-ammonium-coated ONC (NanofilSE3000), or to 6, 18, and 54 µg of a BAC-coated ONC (Nanofil9), and followed for one, 3, or 28 days. All materials induced dose- and time-dependent responses in the exposed mice. However, all doses of Bentonite induced larger, but reversible, inflammation (BAL neutrophils) and acute phase response (Saa3 gene expression in lung) than the two ONC. Similarly, highest levels of DNA strand breaks were found in BAL cells of mice exposed to Bentonite 1 day post-exposure. A significant increase of DNA strand breaks was detected also for NanofilSE3000, 3 days post-exposure. Only mice exposed to Bentonite showed increased Tgf-β gene expression in lung, biomarker of pro-fibrotic processes and hepatic extravasation, 3 days post-exposure. This study indicates that Bentonite treatment with some QAC changes main physical-chemical properties, including shape and surface area, and may decrease their pulmonary toxicity in exposed mice.",
keywords = "Functionalization, occupational health, oxidative stress, particle toxicology, safe by design",
author = "{Di Ianni}, Emilio and Peter M{\o}ller and Alicja Mortensen and J{\'o}zef Szarek and Clausen, {Per Axel} and Saber, {Anne Thoustrup} and Ulla Vogel and Jacobsen, {Nicklas Raun}",
year = "2020",
doi = "10.1080/17435390.2020.1771786",
language = "English",
volume = "14",
pages = "869--892",
journal = "Nanotoxicology",
issn = "1743-5390",
publisher = "Informa Healthcare",
number = "7",

}

RIS

TY - JOUR

T1 - Organomodified nanoclays induce less inflammation, acute phase response, and genotoxicity than pristine nanoclays in mice lungs

AU - Di Ianni, Emilio

AU - Møller, Peter

AU - Mortensen, Alicja

AU - Szarek, Józef

AU - Clausen, Per Axel

AU - Saber, Anne Thoustrup

AU - Vogel, Ulla

AU - Jacobsen, Nicklas Raun

PY - 2020

Y1 - 2020

N2 - Surface modification by different quaternary ammonium compounds (QAC) makes nanoclays more compatible with various polymeric matrices, thereby expanding their potential applications. The growing industrial use of nanoclays could potentially pose a health risk for workers. Here, we assessed how surface modification of nanoclays modulates their pulmonary toxicity. An in vitro screening of the unmodified nanoclay Bentonite (montmorillonite) and four organomodified nanoclays (ONC); coated with various QAC, including benzalkonium chloride (BAC), guided the selection of the materials for the in vivo study. Mice were exposed via a single intratracheal instillation to 18, 54, and 162 µg of unmodified Bentonite or dialkyldimethyl-ammonium-coated ONC (NanofilSE3000), or to 6, 18, and 54 µg of a BAC-coated ONC (Nanofil9), and followed for one, 3, or 28 days. All materials induced dose- and time-dependent responses in the exposed mice. However, all doses of Bentonite induced larger, but reversible, inflammation (BAL neutrophils) and acute phase response (Saa3 gene expression in lung) than the two ONC. Similarly, highest levels of DNA strand breaks were found in BAL cells of mice exposed to Bentonite 1 day post-exposure. A significant increase of DNA strand breaks was detected also for NanofilSE3000, 3 days post-exposure. Only mice exposed to Bentonite showed increased Tgf-β gene expression in lung, biomarker of pro-fibrotic processes and hepatic extravasation, 3 days post-exposure. This study indicates that Bentonite treatment with some QAC changes main physical-chemical properties, including shape and surface area, and may decrease their pulmonary toxicity in exposed mice.

AB - Surface modification by different quaternary ammonium compounds (QAC) makes nanoclays more compatible with various polymeric matrices, thereby expanding their potential applications. The growing industrial use of nanoclays could potentially pose a health risk for workers. Here, we assessed how surface modification of nanoclays modulates their pulmonary toxicity. An in vitro screening of the unmodified nanoclay Bentonite (montmorillonite) and four organomodified nanoclays (ONC); coated with various QAC, including benzalkonium chloride (BAC), guided the selection of the materials for the in vivo study. Mice were exposed via a single intratracheal instillation to 18, 54, and 162 µg of unmodified Bentonite or dialkyldimethyl-ammonium-coated ONC (NanofilSE3000), or to 6, 18, and 54 µg of a BAC-coated ONC (Nanofil9), and followed for one, 3, or 28 days. All materials induced dose- and time-dependent responses in the exposed mice. However, all doses of Bentonite induced larger, but reversible, inflammation (BAL neutrophils) and acute phase response (Saa3 gene expression in lung) than the two ONC. Similarly, highest levels of DNA strand breaks were found in BAL cells of mice exposed to Bentonite 1 day post-exposure. A significant increase of DNA strand breaks was detected also for NanofilSE3000, 3 days post-exposure. Only mice exposed to Bentonite showed increased Tgf-β gene expression in lung, biomarker of pro-fibrotic processes and hepatic extravasation, 3 days post-exposure. This study indicates that Bentonite treatment with some QAC changes main physical-chemical properties, including shape and surface area, and may decrease their pulmonary toxicity in exposed mice.

KW - Functionalization

KW - occupational health

KW - oxidative stress

KW - particle toxicology

KW - safe by design

U2 - 10.1080/17435390.2020.1771786

DO - 10.1080/17435390.2020.1771786

M3 - Journal article

C2 - 32536294

AN - SCOPUS:85086921371

VL - 14

SP - 869

EP - 892

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

IS - 7

ER -

ID: 244690067