P2X7 receptor-deficient mice are susceptible to bone cancer pain

Research output: Contribution to journalJournal articlepeer-review

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P2X7 receptor-deficient mice are susceptible to bone cancer pain. / Hansen, Rikke Rie; Nielsen, Christian K.; Nasser, Arafat; Thomsen, Stine I. M.; Eghorn, Laura F; Pham, Yen; Schulenburg, Cecilia; Syberg, Susanne; Ding, Ming; Stojilkovic, Stanko S; Jorgensen, Niklas R; Heegaard, Anne-Marie.

In: Pain, Vol. 152, No. 8, 2011, p. 1766-76.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Hansen, RR, Nielsen, CK, Nasser, A, Thomsen, SIM, Eghorn, LF, Pham, Y, Schulenburg, C, Syberg, S, Ding, M, Stojilkovic, SS, Jorgensen, NR & Heegaard, A-M 2011, 'P2X7 receptor-deficient mice are susceptible to bone cancer pain', Pain, vol. 152, no. 8, pp. 1766-76. https://doi.org/10.1016/j.pain.2011.03.024

APA

Hansen, R. R., Nielsen, C. K., Nasser, A., Thomsen, S. I. M., Eghorn, L. F., Pham, Y., Schulenburg, C., Syberg, S., Ding, M., Stojilkovic, S. S., Jorgensen, N. R., & Heegaard, A-M. (2011). P2X7 receptor-deficient mice are susceptible to bone cancer pain. Pain, 152(8), 1766-76. https://doi.org/10.1016/j.pain.2011.03.024

Vancouver

Hansen RR, Nielsen CK, Nasser A, Thomsen SIM, Eghorn LF, Pham Y et al. P2X7 receptor-deficient mice are susceptible to bone cancer pain. Pain. 2011;152(8):1766-76. https://doi.org/10.1016/j.pain.2011.03.024

Author

Hansen, Rikke Rie ; Nielsen, Christian K. ; Nasser, Arafat ; Thomsen, Stine I. M. ; Eghorn, Laura F ; Pham, Yen ; Schulenburg, Cecilia ; Syberg, Susanne ; Ding, Ming ; Stojilkovic, Stanko S ; Jorgensen, Niklas R ; Heegaard, Anne-Marie. / P2X7 receptor-deficient mice are susceptible to bone cancer pain. In: Pain. 2011 ; Vol. 152, No. 8. pp. 1766-76.

Bibtex

@article{601b41a7b47e4e49afd291201ec992a6,
title = "P2X7 receptor-deficient mice are susceptible to bone cancer pain",
abstract = "The purinergic P2X7 receptor is implicated in both neuropathic and inflammatory pain, and has been suggested as a possible target in pain treatment. However, the specific role of the P2X7 receptor in bone cancer pain is unknown. We demonstrated that BALB/cJ P2X7 receptor knockout (P2X7R KO) mice were susceptible to bone cancer pain and moreover had an earlier onset of pain-related behaviours compared with cancer-bearing, wild-type mice. Furthermore, acute treatment with the selective P2X7 receptor antagonist, A-438079, failed to alleviate pain-related behaviours in models of bone cancer pain with and without astrocyte activation (BALB/cJ or C3H mice inoculated with 4T1 mammary cancer cells or NCTC 2472 osteosarcoma cells, respectively), suggesting that astrocytic P2X7 receptors play a negligible role in bone cancer pain. The results support the hypothesis that bone cancer pain is a separate pain state compared with those of neuropathic and inflammatory pain. However, the recent discovery of a P2X7 receptor splice variant expressed in the knockout mice used for this study complicates the interpretation of the results. The P2X7 splice variant receptor was detected in the spinal cord but not in osteoclasts of the P2X7R KO mouse. Further experiments are needed to elucidate the exact role of the P2X7 receptors in bone cancer pain.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Hansen, {Rikke Rie} and Nielsen, {Christian K.} and Arafat Nasser and Thomsen, {Stine I. M.} and Eghorn, {Laura F} and Yen Pham and Cecilia Schulenburg and Susanne Syberg and Ming Ding and Stojilkovic, {Stanko S} and Jorgensen, {Niklas R} and Anne-Marie Heegaard",
note = "Copyright {\textcopyright} 2011 International Association for the Study of Pain. All rights reserved.",
year = "2011",
doi = "10.1016/j.pain.2011.03.024",
language = "English",
volume = "152",
pages = "1766--76",
journal = "Pain",
issn = "0304-3959",
publisher = "IASP Press",
number = "8",

}

RIS

TY - JOUR

T1 - P2X7 receptor-deficient mice are susceptible to bone cancer pain

AU - Hansen, Rikke Rie

AU - Nielsen, Christian K.

AU - Nasser, Arafat

AU - Thomsen, Stine I. M.

AU - Eghorn, Laura F

AU - Pham, Yen

AU - Schulenburg, Cecilia

AU - Syberg, Susanne

AU - Ding, Ming

AU - Stojilkovic, Stanko S

AU - Jorgensen, Niklas R

AU - Heegaard, Anne-Marie

N1 - Copyright © 2011 International Association for the Study of Pain. All rights reserved.

PY - 2011

Y1 - 2011

N2 - The purinergic P2X7 receptor is implicated in both neuropathic and inflammatory pain, and has been suggested as a possible target in pain treatment. However, the specific role of the P2X7 receptor in bone cancer pain is unknown. We demonstrated that BALB/cJ P2X7 receptor knockout (P2X7R KO) mice were susceptible to bone cancer pain and moreover had an earlier onset of pain-related behaviours compared with cancer-bearing, wild-type mice. Furthermore, acute treatment with the selective P2X7 receptor antagonist, A-438079, failed to alleviate pain-related behaviours in models of bone cancer pain with and without astrocyte activation (BALB/cJ or C3H mice inoculated with 4T1 mammary cancer cells or NCTC 2472 osteosarcoma cells, respectively), suggesting that astrocytic P2X7 receptors play a negligible role in bone cancer pain. The results support the hypothesis that bone cancer pain is a separate pain state compared with those of neuropathic and inflammatory pain. However, the recent discovery of a P2X7 receptor splice variant expressed in the knockout mice used for this study complicates the interpretation of the results. The P2X7 splice variant receptor was detected in the spinal cord but not in osteoclasts of the P2X7R KO mouse. Further experiments are needed to elucidate the exact role of the P2X7 receptors in bone cancer pain.

AB - The purinergic P2X7 receptor is implicated in both neuropathic and inflammatory pain, and has been suggested as a possible target in pain treatment. However, the specific role of the P2X7 receptor in bone cancer pain is unknown. We demonstrated that BALB/cJ P2X7 receptor knockout (P2X7R KO) mice were susceptible to bone cancer pain and moreover had an earlier onset of pain-related behaviours compared with cancer-bearing, wild-type mice. Furthermore, acute treatment with the selective P2X7 receptor antagonist, A-438079, failed to alleviate pain-related behaviours in models of bone cancer pain with and without astrocyte activation (BALB/cJ or C3H mice inoculated with 4T1 mammary cancer cells or NCTC 2472 osteosarcoma cells, respectively), suggesting that astrocytic P2X7 receptors play a negligible role in bone cancer pain. The results support the hypothesis that bone cancer pain is a separate pain state compared with those of neuropathic and inflammatory pain. However, the recent discovery of a P2X7 receptor splice variant expressed in the knockout mice used for this study complicates the interpretation of the results. The P2X7 splice variant receptor was detected in the spinal cord but not in osteoclasts of the P2X7R KO mouse. Further experiments are needed to elucidate the exact role of the P2X7 receptors in bone cancer pain.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.pain.2011.03.024

DO - 10.1016/j.pain.2011.03.024

M3 - Journal article

C2 - 21565445

VL - 152

SP - 1766

EP - 1776

JO - Pain

JF - Pain

SN - 0304-3959

IS - 8

ER -

ID: 38426119