Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. / Maynard, Scott; Keijzers, Guido; Gram, Martin; Madsen, Claus Desler; Bendix, Laila; Budtz-Jørgensen, Esben; Molbo, Drude; Croteau, Deborah L; Osler, Merete; Stevnsner, Tinna V.; Rasmussen, Lene Juel; Dela, Flemming; Avlund, Kirsten; Bohr, Vilhelm A.

In: Aging, 30.11.2013, p. 850-864.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Maynard, S, Keijzers, G, Gram, M, Madsen, CD, Bendix, L, Budtz-Jørgensen, E, Molbo, D, Croteau, DL, Osler, M, Stevnsner, TV, Rasmussen, LJ, Dela, F, Avlund, K & Bohr, VA 2013, 'Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells', Aging, pp. 850-864.

APA

Maynard, S., Keijzers, G., Gram, M., Madsen, C. D., Bendix, L., Budtz-Jørgensen, E., ... Bohr, V. A. (2013). Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. Aging, 850-864.

Vancouver

Maynard S, Keijzers G, Gram M, Madsen CD, Bendix L, Budtz-Jørgensen E et al. Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. Aging. 2013 Nov 30;850-864.

Author

Maynard, Scott ; Keijzers, Guido ; Gram, Martin ; Madsen, Claus Desler ; Bendix, Laila ; Budtz-Jørgensen, Esben ; Molbo, Drude ; Croteau, Deborah L ; Osler, Merete ; Stevnsner, Tinna V. ; Rasmussen, Lene Juel ; Dela, Flemming ; Avlund, Kirsten ; Bohr, Vilhelm A. / Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. In: Aging. 2013 ; pp. 850-864.

Bibtex

@article{26e655d8238647db9e161247cffac2ce,
title = "Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells",
abstract = "Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.",
author = "Scott Maynard and Guido Keijzers and Martin Gram and Madsen, {Claus Desler} and Laila Bendix and Esben Budtz-J{\o}rgensen and Drude Molbo and Croteau, {Deborah L} and Merete Osler and Stevnsner, {Tinna V.} and Rasmussen, {Lene Juel} and Flemming Dela and Kirsten Avlund and Bohr, {Vilhelm A}",
year = "2013",
month = "11",
day = "30",
language = "English",
pages = "850--864",
journal = "Aging",
issn = "1945-4589",
publisher = "Impact Journals LLC",

}

RIS

TY - JOUR

T1 - Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

AU - Maynard, Scott

AU - Keijzers, Guido

AU - Gram, Martin

AU - Madsen, Claus Desler

AU - Bendix, Laila

AU - Budtz-Jørgensen, Esben

AU - Molbo, Drude

AU - Croteau, Deborah L

AU - Osler, Merete

AU - Stevnsner, Tinna V.

AU - Rasmussen, Lene Juel

AU - Dela, Flemming

AU - Avlund, Kirsten

AU - Bohr, Vilhelm A

PY - 2013/11/30

Y1 - 2013/11/30

N2 - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

AB - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

M3 - Journal article

SP - 850

EP - 864

JO - Aging

JF - Aging

SN - 1945-4589

ER -

ID: 94050456