Reversibility of exercise-induced translocation of Na+-K+ pump subunits to the plasma membrane in rat skeletal muscle
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Reversibility of exercise-induced translocation of Na+-K+ pump subunits to the plasma membrane in rat skeletal muscle. / Juel, C; Grunnet, L; Holse, M; Kenworthy, S; Sommer, V; Wulff, T.
In: Pflügers Archiv - European Journal of Physiology, Vol. 443, No. 2, 11.2001, p. 212-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reversibility of exercise-induced translocation of Na+-K+ pump subunits to the plasma membrane in rat skeletal muscle
AU - Juel, C
AU - Grunnet, L
AU - Holse, M
AU - Kenworthy, S
AU - Sommer, V
AU - Wulff, T
PY - 2001/11
Y1 - 2001/11
N2 - Exercise-induced translocation of Na+-K+ pump subunits to the sarcolemmal membrane was studied using sarcolemmal giant vesicles as a membrane purification procedure. The subunit content was quantified by Western blotting or by ouabain labeling. Low-intensity treadmill running increased (P<0.01) the alpha1, alpha2, beta1, and beta2 subunit contents by 19-32% in membranes from oxidative muscle fibers and the alpha1, alpha2, and beta2 contents increased by 13-25% in membranes from glycolytic muscle fibers. Ouabain labeling of membranes from mixed fibers was increased by 29% after exercise. A similar increase in subunit content could be induced by 5 min of fatiguing, high-intensity electrical stimulation of isolated soleus muscles. An increased subunit content was just detectable in vesicles produced 30 min after exercise, and the content was completely back to control levels 3 h after exercise. It is concluded that both low-intensity long-lasting running and short-lasting high-intensity contractions are able to induce a translocation of pump subunits to the sarcolemmal membrane. The post-exercise disappearance of the extra subunits (half-time approximately 20 min) from the membrane demonstrates the reversible nature of the translocation process.
AB - Exercise-induced translocation of Na+-K+ pump subunits to the sarcolemmal membrane was studied using sarcolemmal giant vesicles as a membrane purification procedure. The subunit content was quantified by Western blotting or by ouabain labeling. Low-intensity treadmill running increased (P<0.01) the alpha1, alpha2, beta1, and beta2 subunit contents by 19-32% in membranes from oxidative muscle fibers and the alpha1, alpha2, and beta2 contents increased by 13-25% in membranes from glycolytic muscle fibers. Ouabain labeling of membranes from mixed fibers was increased by 29% after exercise. A similar increase in subunit content could be induced by 5 min of fatiguing, high-intensity electrical stimulation of isolated soleus muscles. An increased subunit content was just detectable in vesicles produced 30 min after exercise, and the content was completely back to control levels 3 h after exercise. It is concluded that both low-intensity long-lasting running and short-lasting high-intensity contractions are able to induce a translocation of pump subunits to the sarcolemmal membrane. The post-exercise disappearance of the extra subunits (half-time approximately 20 min) from the membrane demonstrates the reversible nature of the translocation process.
KW - Animals
KW - Biological Transport
KW - Cell Membrane/metabolism
KW - Electric Stimulation
KW - Glycolysis
KW - Male
KW - Motor Activity/physiology
KW - Muscle, Skeletal/metabolism
KW - Ouabain/metabolism
KW - Oxidation-Reduction
KW - Rats
KW - Rats, Wistar
KW - Sodium-Potassium-Exchanging ATPase/metabolism
KW - Time Factors
U2 - 10.1007/s004240100674
DO - 10.1007/s004240100674
M3 - Journal article
C2 - 11713646
VL - 443
SP - 212
EP - 217
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
SN - 0031-6768
IS - 2
ER -
ID: 210980914