TY - JOUR

T1 - Sex, food, and the gut microbiota: Disparate response to caloric restriction diet with fibre supplementation in women and men

AU - Benítez-Páez, Alfonso

AU - Hess, Anne Lundby

AU - Krautbauer, Sabrina

AU - Liebisch, Gerhard

AU - Christensen, Lars

AU - Hjorth, Mads Fiil

AU - Larsen, Thomas Meinert

AU - Sanz, Yolanda

AU - MyNewGut Consortium

N1 - This article is protected by copyright. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Scope: Dietary-based strategies are regularly explored in controlled clinical trials to provide cost-effective therapies to tackle obesity and its comorbidities. We present a complementary analysis based on a multivariate multi-omics approach of a caloric restriction intervention with fibre supplementation to unveil synergic effects on body weight control, lipid metabolism, and gut microbiota.Methods and results: We explored faecal BAs and SCFAs, plasma BAs, and faecal shotgun metagenomics on 80 overweight participants of a 12-week caloric restriction clinical trial (-500 kcal/day) randomly allocated into fibre (10 g/day inulin + 10 g/day resistant maltodextrin) or placebo (maltodextrin) supplementation groups. The multi-omic data integration analysis (sparse PLS-DA method) uncovered the benefits of the fibre supplementation and/or the CRD (e.g., increase of Parabacteroides distasonis and faecal propionate), showing sex-specific effects on either adiposity and fasting insulin; effects thought to be linked to changes of specific gut microbiota species, functional genes, and bacterially produced metabolites like SCFAs and secondary BAs.Conclusions: Our study has identified diet-microbe-host interactions helping to design personalised interventions. It also suggests that sex perspective should be considered routinely in future studies on dietary interventions efficacy. All in all, we uncovered that our dietary intervention was more beneficial for women than men. This article is protected by copyright. All rights reserved.

AB - Scope: Dietary-based strategies are regularly explored in controlled clinical trials to provide cost-effective therapies to tackle obesity and its comorbidities. We present a complementary analysis based on a multivariate multi-omics approach of a caloric restriction intervention with fibre supplementation to unveil synergic effects on body weight control, lipid metabolism, and gut microbiota.Methods and results: We explored faecal BAs and SCFAs, plasma BAs, and faecal shotgun metagenomics on 80 overweight participants of a 12-week caloric restriction clinical trial (-500 kcal/day) randomly allocated into fibre (10 g/day inulin + 10 g/day resistant maltodextrin) or placebo (maltodextrin) supplementation groups. The multi-omic data integration analysis (sparse PLS-DA method) uncovered the benefits of the fibre supplementation and/or the CRD (e.g., increase of Parabacteroides distasonis and faecal propionate), showing sex-specific effects on either adiposity and fasting insulin; effects thought to be linked to changes of specific gut microbiota species, functional genes, and bacterially produced metabolites like SCFAs and secondary BAs.Conclusions: Our study has identified diet-microbe-host interactions helping to design personalised interventions. It also suggests that sex perspective should be considered routinely in future studies on dietary interventions efficacy. All in all, we uncovered that our dietary intervention was more beneficial for women than men. This article is protected by copyright. All rights reserved.

KW - Faculty of Science

KW - Dietary fibre

KW - Gut microbiome

KW - Metabolomics

KW - Inulin

KW - Resistant maltodextrin

KW - Obesity

KW - Weight loss

KW - Multi-omics

U2 - 10.1002/mnfr.202000996

DO - 10.1002/mnfr.202000996

M3 - Journal article

C2 - 33629506

VL - 65

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 8

M1 - 2000996

ER -